Attenuating effect of drugs on an impairment of anticipatory activity in various animal models.

药物对各种动物模型预期活动损害的减弱作用。

• 批准号: 05671900
• 负责人: SHIBATA Shigenobu
• 金额: $ 1.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671900/
• 关键词: Circadian Rhythms; Anticipation; Entrainment; Suprachiasmatic; Acetylcholine; Time cognition; Learning; Activity; 体内時計; 学習・記憶; 夜間徘徊; 生体リズム

The suprachiasmatic nucleus (SCN) has been identified as a pacemaker for mammalian circadian rhythms. There are two different entraining pathways : one is retinohypothalamic tract and the othere is geniclohypothalamic tract. As glutamate has been suggested as a transmitter of the retinohypothalamic tract, we examined the effect of NMDA,a glutamate NMDA receptor agonist on the SCN neuronal activity thythem. NMDA produced phase changes similar to light-induced phase shifts through the nitric oxide production in the SCN.In addition, AMPA,an ionotropic glutamate receptor agonist caussed similar phase shifts via the activation of protein synthesis.Restricted feeding at a fixed time of the day produced an entrainment in hamsters and rats. At present, the neuronal mechanism of this anticipatory food-entrainable rhythms are uncertain. The food-and methamphetamine-associated anticipation were impaired by aging and a treatment with NMDA receptor antagonist, MK-801. The impairment of this anticipation in aging animals was attenuated by cholinergic drugs such as arecoline and physostigmine. In addition, daily lever pressing at a fixed time of day to drink water was also established in operant experiment paradgim. These results suggest that cholinergic drugs may be important in the performance of the anticipatory activity rhythms.

视交叉上核（SCN）已被确定为哺乳动物昼夜节律的起搏器。有两种不同的牵引途径：一种是视网膜下丘脑束，另一种是膝下丘脑束。由于谷氨酸被认为是视网膜下丘脑束的一种递质，我们研究了NMDA，一种谷氨酸NMDA受体激动剂对SCN神经元活性的影响。NMDA通过SCN中一氧化氮的产生产生类似于光诱导相移的相变。此外，AMPA，一种离子型谷氨酸受体激动剂，通过激活蛋白质合成引起类似的相移。在一天的固定时间限制进食，在仓鼠和老鼠中产生了一种娱乐。目前，这种预期的食物可吸收节律的神经元机制尚不确定。随着年龄的增长和NMDA受体拮抗剂MK-801的治疗，与食物和甲基苯丙胺相关的预期受损。在衰老的动物中，这种预期的损害被胆碱能药物如槟榔碱和芥子碱所减弱。此外，在操作实验范式中还建立了每日固定时间按杠杆喝水的方法。这些结果表明，胆碱能药物可能在预期活动节律的表现中起重要作用。

项目成果

C.Fukuhara: "Phose adronces of incadion rlythns in somatostatin clepletid rats:effects of cysteontne on rluythns of locomot activssty and electrical dischorses of cleccd" J.Com.Physiology A. 175. 677-685 (1994)

C.Fukuhara：“生长抑素 clepletid 大鼠中 incadion 节律的Phose adronces：半胱氨酸对 cleccd 运动节律和电紊乱的影响” J.Com.Physiology A. 175. 677-685 (1994)

Shibata,S.: "Tetrodotoxin does not affect circadian rhythms neuronal activity and metabolism in rodent suprachianatu mucleus in vitro" Brain Research. 606. 259-266 (1993)

Shibata,S.：“河豚毒素不会影响体外啮齿动物上粘膜的昼夜节律神经元活动和代谢”大脑研究。

Shigenobu Shibata: "Calmodulin inhibitors produce phase shifts of circadian rhythms in vivo and in vitro." Journal of Biological Rhythms. 9. 27-41 (1994)

Shigenobu Shibata：“钙调蛋白抑制剂在体内和体外产生昼夜节律的相移。”

Shibata,S.: "Age-related impairment of food-anticipatory locomotor activity rhythm in rats" Physilogy and Behavior. (in press). (1994)

Shibata，S.：“大鼠食物预期运动活动节律的年龄相关损伤”生理学和行为。

Shigenobu Shibata: "Aging impairs methamphetamine-induced free-running and anticipatory locomotor activity rhythms in rats." Neuroscience Letters. 172. 107-110 (1994)

Shigenobu Shibata：“衰老会损害甲基苯丙胺诱导的大鼠自由奔跑和预期运动活动节律。”