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Clock gene response against skelton type entrainment stimulation in mice

小鼠中针对骨架型夹带刺激的时钟基因反应

基本信息

批准号：
18390071
负责人：
SHIBATA Shigenobu
金额：
$ 10.21万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390071/
关键词：
circadian clock gene food anticipation liver light entrainment nutrition chrononutrition circadian entrainment food anticipation dexamethasone mouse

项目摘要

The aim of this experiment is to elucidate the mechanism of skeleton-type photic and non-photic entrainment like food entrainment in mice. First we examined the circadian pattern of clock gene expression in the liver under one-hr light stimulation in the morning and one-hr light stimulation in the evening. This lighting schedule could entrain the locomotor activity rhythm and also liver dock gene expression rhythm. Thus, skeleton lighting schedule can entrain the circadian dock just like usual 12-hr light-dark schedule. Next, we examined the circadian pattern of clock gene expression in the liver under 2-hr feeding in the morning and 2-hr feeding in the evening. This feeding schedule could entrain the liver clock gene expression rhythm, and its pattern was very similar to adlib feeding group. Thus, two feeding times per day with morning and evening could entrain the peripheral circadian rhythm. In the third experiment, we changed the ratio of feeding volume in the morning and evening. If the more feeding volume was applied in the morning, there was little effect on the phase of dock gene expression. However, if the more feeding volume was applied in the evening, phase of dock gene expression rhythm was strongly delayed. Taken together these results suggest that food intake in the morning is important to keep the phase of dock gene expression the peripheral organs.

本实验的目的是阐明小鼠骨骼型光和非光夹带（如食物夹带）的机制。首先，我们检查了早晨一小时光刺激和晚上一小时光刺激下肝脏中时钟基因表达的昼夜节律模式。这种照明时间表可以控制运动活动节律以及肝脏码头基因表达节律。因此，骨架照明时间表可以像通常的 12 小时明暗时间表一样引导昼夜节律码头。接下来，我们检查了早晨 2 小时喂养和晚上 2 小时喂养下肝脏中时钟基因表达的昼夜节律模式。这种喂养方案可以带动肝脏时钟基因的表达节律，其模式与adlib喂养组非常相似。因此，每天早上和晚上两次喂食可以控制周围的昼夜节律。在第三个实验中，我们改变了早上和晚上的饲喂量比例。如果早上施加更多的饲喂量，则对码头基因表达的阶段影响很小。然而，如果在晚上施加更多的饲喂量，则码头基因表达节律的阶段被强烈延迟。综上所述，这些结果表明早上的食物摄入对于保持外周器官的码头基因表达阶段很重要。