Physiological Study of Pungent Agents Extracted from Various Spices in Rat and Guinea Pig

各种香料提取的辛辣剂对大鼠和豚鼠的生理研究

• 批准号: 05680043
• 负责人: AOMINE Masahiro
• 金额: $ 1.15万
• 依托单位: Nakamura Gakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680043/
• 关键词: Spice; Pungent agent; Physiology; Rat; Guinea pig; Heart; Uterus; Respiration; Contraction; Blood flow

In this study we used capsaicin, piperine, and diallyl disulfide as pungent agents extracted from spices. We did next three kinds of experiments to clarify unknown direct or indirect physiological effects of these agents, using a whole body or isolated preparation of heart and uterus of rats and guinea pigs.Study 1 : Effects of capsaicin on isolated ventricular papillary muscle from rat and guinea pig. Capsaicin showed dual effects on the contractile tension of rat papillary muscle. That is, the low concentration (0.1muM) increased the amplitude of tension, but the high concentration (*10muM) decreased it. The increase in the tension at low concentration never happened in the presence of beta-blocker, propranolol. Effective concentration 50% (EC_<50>) of capsaicin in the amplitude of tension at 1Hz was around 10muM.On the other hand, all concentrations used decreased the tension of rat papillary muscle in a concentration-dependent manner. EC_<50> at 1Hz was around 0.1muM,showing a high … More sensitivity to capsaicin in rat. In the same animal, we investigated the effects of capsaicin on the rested-state contraction (RSC) to know its electrophysiological actions on intracellular Ca store, and compared the findings with effects of caffeine and ryanodine, because RSC is believed to be mostly dependent on extra Ca released from the Ca store. As a result, capsaicin had no direct effect on the Ca store. Our data suggest that capsaicin-induced negative inotropic action is largely due to a block of Ca channels, as we have reported previously, using isolated single ventricular cells (Jpn.J.Physiol.43 [Suppl.2] : s155,1993).Study 2 : Comparison of contractile effects between capsaicin, piperine and diallyl disulfide in isolated rat myometriumAll three agents, capsaicin, piperine and diallyl disulfide repressed the amplitude and frequency of spontaneous contraction of isolated rat myometrium. Diallyl disulfide was the most effective in inhibiting the amplitude of the tension, followed by piperine and capsaicin in that order. EC_<50> 's in inhibiting the amplitude were 20muM in diallyl disulfide, 50muM in piperine and 0.1mM in capsaicin. EC_<50> 's in inhibiting the frequency were 20muM in diallyl disulfide and 0.1mM in capsaicin. Piperine showed no or little effect on the frequency. It is suggested that these agents decrease the tension, via a block of Ca channels.Study 3 : Effects of capsaicin, piperine and diallyl disulfide on the respiration and periferal blood flow of diabetic and non-diabetic ratsIn olfactory experiments, three agents increased the number of respiration per minute and periferal blood flow in the abdomen of spontaneous diabetic rats, while they had no or little effect in non-diabetic ones. In taste experiments, three agents produced a little increase in the respiration of non-diabetic rats, but these produced a decrease in that of diabetics. The blood flow in diabetic rats was decreased by three agents, but that in non-diabetic rats was not effected. To elucidate these experimental facts, we need further experiments. Less

在这项研究中，我们使用辣椒素，胡椒碱，二烯丙基二硫化物作为辛辣剂提取的香料。我们做了以下三种实验，以澄清未知的直接或间接的生理作用，这些代理商，使用整个身体或分离的制备的心脏和子宫的大鼠和豚鼠。研究1：辣椒素对大鼠和豚鼠的心室乳头肌的影响。辣椒素对大鼠乳头肌收缩张力具有双重作用。即低浓度（0.1 μ M）使张力幅值增加，而高浓度（10 μ M）使张力幅值减小，而β-受体阻滞剂普萘洛尔在低浓度时不引起张力的增加。辣椒<50>素在1Hz时对心室肌张力的50%有效浓度（EC_（50））约为10 μ M。EC_<50>（1Hz）约为0.1 μ M， ...更多信息 大鼠对辣椒素敏感性在同一动物中，我们研究了辣椒素对静息状态收缩（RSC）的影响，以了解其对细胞内钙库的电生理作用，并将研究结果与咖啡因和ryanodine的作用进行了比较，因为RSC被认为主要依赖于从钙库释放的额外钙。因此，辣椒素对钙库没有直接影响。我们的数据表明，辣椒素诱导的负性肌力作用主要是由于钙通道的阻断，正如我们以前使用分离的单个心室细胞报道的那样（Jpn.J.Physiol.43 [Suppl.2]：s155，1993）。辣椒素、胡椒碱和二烯丙基二硫对离体大鼠子宫肌收缩作用的比较胡椒碱和二烯丙基二硫抑制离体大鼠子宫肌层自发收缩的幅度和频率。二烯丙基二硫对张力的抑制作用最强，胡椒碱次之，辣椒碱最弱。二<50>烯丙基二硫、胡椒碱和辣椒素对该酶的抑制作用分别为20 μ M、50 μ M和0.1mM。二<50>烯丙基二硫抑制频率的EC值为20 μ M，辣椒素为0.1mM。苦参碱对频率无影响或影响很小。研究3：辣椒素、胡椒碱和二烯丙基二硫化物对糖尿病和非糖尿病大鼠呼吸和外周血流量的影响在嗅觉实验中，三种药物均能增加自发性糖尿病大鼠的每分钟呼吸次数和腹部外周血流量，而对非糖尿病大鼠无影响或影响很小。在味觉实验中，三种药物使非糖尿病大鼠的呼吸略有增加，但使糖尿病大鼠的呼吸减少。三种药物均能降低糖尿病大鼠的血流量，而对非糖尿病大鼠的血流量无明显影响。为了阐明这些实验事实，我们需要进一步的实验。少

项目成果

Yamato,T.,Aomine,M.et al: "Capsaicin does not inhibit the intracellular calcium handling process in rat papillary muscle" General Phamacology. 26(in press).

Yamato,T.,Aomine,M.等人：“辣椒素不会抑制大鼠乳头肌的细胞内钙处理过程”普通药理学。

Yamato,T.,Aomine,M.et al: "Inhibition of contractile tension by capsaicin in isolated rat papillary muscle" General Phamacology. 26(in press).

Yamato,T.,Aomine,M.等人：“辣椒素对离体大鼠乳头肌收缩张力的抑制”普通药理学。

Yamato, T., Aomine, M., Ikeda, M., Noto, H.and Ohta, C.: "Inhibition of contractile tension by capsaicin in isolated rat papillary muscle" General Phamacology. 26(in press).

Yamato, T.、Aomine, M.、Ikeda, M.、Noto, H. 和 Ohta, C.：“辣椒素对离体大鼠乳头肌收缩张力的抑制”普通药理学。

Yamato, T., Aomine, M., Ikeda, M., Noto, H.and Ohta, C.: "Capsaicin dose not inhibit the intracellular calcium handing process in rat papillary muscle" General Phamacology. 26(in press).

Yamato, T.、Aomine, M.、Ikeda, M.、Noto, H. 和 Ohta, C.：“辣椒素不会抑制大鼠乳头肌的细胞内钙处理过程”普通药理学。