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Does nutrients modulate food-intake behavior? Measurement of neurotransmitters using in vivo brain microdialysis in diabetic rat.

营养素是否会调节食物摄入行为？

基本信息

批准号：
10680157
负责人：
AOMINE Masahiro
金额：
$ 2.24万
依托单位：
Nakamura Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In the present work, the following studies were undertaken : (Exp.1) Whether the abnormal behavior of food intake in diabetic animals just like human diabetic patients exists or not ; (Exp.2) Whether the abnormality of food-intake behavior will be responsible for the altered release of neurotransmitters in the diabetic brain or not, presumably permitting the frailty to stressors ; (Exp.3) Whether among many foods coffee and its main components are effective in stress-ralaxation or not. (Exp.1) WBN/Kob rat and KK/Ta mouse as spontaneously diabetic animal, and normal Wistar-rats and C57/BL mouse as thc control were used. In diabetic animals the spontaneous locomotor activity decreased. In both normal and diabetic animals, acute stress (100 min-restraint) elicited the decrease in food- and water-intake, and the circadian rhythm of food- and water-intake was disturbed especially in diabetic mice. (Exp.2) In this experiment the hippocampal levels of serotonin (5-HT) and dopamine (DA) of WBN … More /Kob rat, streptozotocin (STZ)-induced diabetic rat and normal Wistar rat were measured using in vivo brain microdialysis method. The 5-HT level in normal Wistar rat was the largest value, followed by that in STZ-rat and WBN/Kob rat in that order. The 5-HT level in WBN/Kob rat was about a half of that of the normal rat, and the DA level in WBN/Kob rat was about one-tenth of that of the normal rat. The experiment using several ion channel blockers suggested the dysfunction of ion channels in diabetic synapse. (Exp.3) Acute restraint stress led to prominent increase in the hippocampal levels of 5-HT.In normal rat, "peak 5-HT value during 2nd restraint/peak 5-HT value during 1st restraint" (stress-ralaxing index) in pretreatment of coffee, caffeine, chlorogenic acid and saline was about 37%, 34%, 130% and 85%, respectively. This suggests that the administration of coffee contributes to relaxation of restraint stress and further this effect of coffee could be due to the caffeine which is contained in the coffee. On the other hand, in WBN rat, the stress-ralaxing index in pretreatment of coffee, caffeine, and saline was about 45%, 51%, 68% and 85%, respectively, suggesting the frailty to stressors in diabetic animals. Less

在目前的工作中，进行了以下研究：（实验1）是否存在与人类糖尿病患者一样的糖尿病动物的异常摄食行为;（实验2）进食行为的异常是否会导致糖尿病患者脑内神经递质释放的改变，从而可能导致对应激源的脆弱性;（实验3）在众多食物中，咖啡及其主要成分是否具有缓解压力的作用。（实验1）WBN/Kob大鼠和KK/Ta小鼠作为自发性糖尿病动物，正常Wistar大鼠和C57/BL小鼠作为对照。在糖尿病动物中，自发运动活动减少。在正常和糖尿病动物中，急性应激（100分钟限制）引起的食物和水的摄入量减少，食物和水的摄入量的昼夜节律被打乱，特别是在糖尿病小鼠。实验二：观察了WBN大鼠海马5-HT和DA含量的变化， ...更多信息采用在体脑微透析技术，对糖尿病大鼠、正常Wistar大鼠和链脲佐菌素（STZ）诱导的糖尿病大鼠进行了脑微透析检测。正常Wistar大鼠5-HT含量最高，其次为STZ大鼠和WBN/Kob大鼠。WBN/Kob大鼠5-HT含量约为正常大鼠的一半，DA含量约为正常大鼠的十分之一。多种离子通道阻断剂的应用提示糖尿病突触的离子通道功能障碍。（实验3）急性束缚应激导致海马5-HT水平显著升高。在正常大鼠中，咖啡、咖啡因、绿原酸和盐水预处理的“第二次束缚期间的5-HT峰值/第一次束缚期间的5-HT峰值”（应激松弛指数）分别约为37%、34%、130%和85%。这表明咖啡有助于放松束缚压力，咖啡的这种作用可能是由于咖啡中含有的咖啡因。另一方面，在WBN大鼠中，咖啡、咖啡因和盐水预处理的应激松弛指数分别约为45%、51%、68%和85%，表明糖尿病动物对应激源的脆弱性。少