Development of New Sensing Methods for Bioactive Ions and Molecules by Mimicking Homeostasis in Biological Membranes

通过模拟生物膜内稳态开发生物活性离子和分子的新传感方法

• 批准号: 06453067
• 负责人: SUGAWARA Masao
• 金额: $ 4.61万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453067/
• 关键词: Calmodulin; synthetic peptide; surface plasmon resonance; active transport; dopamine; Lasalocid A; planar bilayer; synthetic receptor; ホメオスタシス; M13; カルシウムセンサ; バイオセンサー

New Sensing Membranes based on the principle of homeostasis have been developed.1) An optical sensor has been described by using calmodulin (isolated and purified from calf brain) and a synthetic peptide M13. Formation of a ternary complex between Ca^<2+>/calmodulin and M13 was monitored by using the surface plasomon resonance method. A highly sensitive and selective detection of several tens nM Ca^<2+> ions was demonstrated.2) An active transport system for dopamine was constructed by using lasalocid as a carrier dissolved in liquid membranes. Enhancement of concetratio of dopamine at nM levels was achived by using the active transport system.3) Direct incorporation of synthetic receptors into planar bilayer lipid membranes formed by the folding method was successfully achieved. New potentiometric sensing systems were designed by using these receptor-incorporated BLMs.

基于稳态原理的新型传感膜已经开发出来。1）利用钙调素（从小牛脑中分离纯化）和合成肽M13描述了一种光学传感器。用表面等离子体共振方法监测Ca^<2+>/钙调素与M13之间三元复合物的形成。2）以拉沙洛西为载体，构建了多巴胺的主动转运体系。通过主动转运系统，多巴胺的浓度提高到了nM水平。3）成功地将合成受体直接掺入到折叠法形成的平面双层脂膜中。利用这些结合受体的BLM设计了新的电位传感系统。

项目成果

N.Namba: "Molecular resolution images of a calix[6]arene from atomic force microscopy" Langmuir. 11(in press). (1995)

N.Namba：“原子力显微镜下杯[6]芳烃的分子分辨率图像”Langmuir。

M.Sugawara: "Frontiers in Biosensors" F.D.Sheller(ed.)(in press), (1996)

M.菅原：“生物传感器的前沿”F.D.Sheller（编辑）（印刷中），（1996）

M.Sugawara: "Current Topics in Biophysics" 未定, (1995)

M.Sukawara：“生物物理学的当前主题”TBA，（1995）

M.Sugawara: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier" Anal.Sci.(in press). (1996)

M.Sukawara：“使用拉沙洛西德 A 作为载体通过质子驱动的上坡运输来浓缩多巴胺”Anal.Sci.（出版中）。

M.Sugawara, H.Sato, T.Ozawa, Y.Umezawa: Receptor Based Chemical Sensing, in Frontiers in Biosensors. F.D.Scheller (in press),

M.Sukawara、H.Sato、T.Ozawa、Y.Umezawa：基于受体的化学传感，生物传感器前沿。