Development of New Sensing Methods for Bioactive Ions and Molecules by Mimicking Homeostasis in Biological Membranes

基于稳态原理的新型传感膜已被开发出来。1)利用从小牛脑中分离纯化的钙调蛋白和合成肽M13制备了一种光学传感器。用表面等离子体共振法监测了Ca^<2+>/钙调素与M13之间三元配合物的形成。证明了对数十nM Ca^<2+ b>离子的高灵敏度和选择性检测。2)以喇沙酸为载体，溶解于液膜中，构建了多巴胺主动转运系统。通过主动转运系统，多巴胺浓度达到nM水平。3)成功地将合成受体直接结合到折叠法形成的平面双层脂膜中。利用这些受体结合的blm设计了新的电位传感系统。

New Sensing Membranes based on the principle of homeostasis have been developed.1) An optical sensor has been described by using calmodulin (isolated and purified from calf brain) and a synthetic peptide M13. Formation of a ternary complex between Ca^<2+>/calmodulin and M13 was monitored by using the surface plasomon resonance method. A highly sensitive and selective detection of several tens nM Ca^<2+> ions was demonstrated.2) An active transport system for dopamine was constructed by using lasalocid as a carrier dissolved in liquid membranes. Enhancement of concetratio of dopamine at nM levels was achived by using the active transport system.3) Direct incorporation of synthetic receptors into planar bilayer lipid membranes formed by the folding method was successfully achieved. New potentiometric sensing systems were designed by using these receptor-incorporated BLMs.