Isolation and purification of antifeedant and antioviposition substances against the diamondback moth from a tropical plant.

从热带植物中分离和纯化小菜蛾拒食和抗产卵物质。

• 批准号: 06454062
• 负责人: NAKASUJI Fusao
• 金额: $ 0.38万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454062/
• 关键词: substance of tropical plant; Andrographis paniculata; diamondback moth; Plutella xylostella; antifeedant; antioviposition; Andrographis paniculata

In 1994, antifeedant activity of the acetone extract from A.paniculata against the 1st- and 4th-instar larvae of the diamondback moth (DBM), P.xylostella, was observed at 60 ppm and 500 ppm respectively. Antioviposition effect on the DBM females was also observed on caged cabbage treated with these extracts at 125 ppm.In 1995, a series of fractionation of the aerial parts of A.paniculata guided by a no-choice bioassay test on the 4th-instar larvae of the DBM was conducted for the isolation of the hexane, ETOAc, BuOH and water layrs. Subsequent bioassay tests showed that the hexane layr is the most potent antifeedant against the DBM larvae. Futher fractionation of the hexane layr yielded 7 fractions. Fractions 5 and 6 were still effective even at a reduced dose (50 mug/leaf disc). Thus, fraction 5 was subsequently fractionated and 5 sub-fractions (F5-1 to F5-5) were obtained. Among those F5-2 was the most effective feeding deterrent at a minimum dose of 25mug/leaf disc. Through preparative TLC,an active compound (F526) was finally purified and isolated from F5-2. Further chemical analysis using other analytical procedures will be done to determine the chemical structure of this active compound.

在1994年，分别在60 ppm和500 ppm的情况下观察到了来自a. paniculata的丙酮提取物对钻石飞蛾（DBM）的第一和第4个星级幼虫（DBM），P.xylostella的抗源性活性。还观察到在1995年第125页的笼中用这些提取物处理的cabbage对DBM女性的抗反应作用，这是A. paniculata空中部分的一系列分馏，该a.paniculata的空中部位由无选择的生物测定试验以DBM的第4个标准幼虫的繁殖而进行，以供DBM的lay lay和Sibles sexane sipals and sexane and sibles。随后的生物测定测试表明，己烷Layr是针对DBM幼虫的最有效的抗铁蛋白。己烷Layr的未分馏产生了7个分数。即使在减少剂量（50杯/叶盘）下，分数5和6仍然有效。因此，随后将分数分馏出来，并获得了5个亚裂缝（F5-1至F5-5）。在这些F5-2中，最有效的进食威慑力量，最小剂量为25mug/叶盘。通过制剂TLC，最终将活性化合物（F526）从F5-2分离出来。将采用其他分析程序进行进一步的化学分析，以确定该活性化合物的化学结构。

项目成果

Hermawan, W., S.Kajiyama, R.Tsukuda, K.Fujisaki, A.Kobayashi and F.Nakasuji: "Antifeedant and antioviposition activities of the extract from a tropical plant, Andrographispaniculata (Acanthaceac), against the diamondback moth, Plutella xylostella (Lepidop

Hermawan，W.，S.Kajiyama，R.Tsukuda，K.Fujisaki，A.Kobayashi 和 F.Nakasuji：“热带植物穿心莲（Acanthaceac）提取物对小菜蛾（Plutella xylostella）的拒食和抗产卵活性

Herwawan,W.et al.: "Antifeedant and antioviposition activities of the fractions of extrct from a tropical plant,Andrographis paniculata(Acan thaceae),against the diamondback moth,Plutella xylostella(Lepidoptera:Yponomeutidae)" Applied Entomology and Zoolo

Herwawan，W.等人：“热带植物穿心莲（Acan thaceae）提取物的组分对小菜蛾、小菜蛾（鳞翅目：Yponomeutidae）的抗进食和抗产卵活性”应用昆虫学和动物学

Hermawan, W., S. kajiyama, R. Tsukuda, K. Fujisaki, A. Kobayashi and F. Nakasuji: "Antifeedant and antioviposition activities of the fractions of extract from a tropical plant, Andrographis paniculata (Acanthaceae), against the diamondback moth, Plutella

Hermawan, W., S. kajiyama, R. Tsukuda, K. Fujisaki, A. Kobayashi 和 F. Nakasuji：“热带植物穿心莲（棘科）提取物的组分对小菜蛾的抗进食和抗产卵活性