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Mechanism of physiological resurgence affecting recovery of susceptibility in fenvalerate-resistant strains of the diamondback moth Plutella xylostella.

生理复苏影响小菜蛾抗氰戊菊酯菌株敏感性恢复的机制。

基本信息

批准号：
14360029
负责人：
NAKASUJI Fusao
金额：
$ 5.82万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2005
项目状态：
已结题

项目摘要

A diamondback moth (DBM) strain resistant to fenvalerate was established and the effects of sublethal dose of the insect on egg size and fecundity were examined. The eggs of the selected strain were significantly smaller than those of non-selected strain. The fecundity of females of the selected strain tended to be higher than that of the non-selected strain. The survival rate of the selected strain was lower than that of the non-selected strain.To determine mode of inheritance of resistant development and of reduction of egg size in DBM, F_1 offspring of reciprocal crosses were compared with their parental lines for these traits. Several fitness components were also compared between F1 offspring of reciprocal crosses as well as the resistant and susceptible lines to detect maternal effects on the components including egg size. The dose-mortality regression lines of the F_1 hybrid of susceptible females and resistant males (SR) and resistant females and susceptible males (RS) were roug … More hly intermediate between that of parental lines, i.e., SS and RR. This result indicates that the mode of inheritance of fenvalerate resistance was neither complete dominant nor recessive with no sex-linkage. The egg size of RR lines was significantly smaller than that of the SS lines. The RS and SR lines have an egg size close to that of their own matrilineage. It was considered that the mode of inheritance of resistance and that of egg size were different from each other, although the selection experiment showed that there is a negative correlated response between these parameters.The recovery of susceptibility to fenvarelate in resistant strains of DBM was examined through 10 generations after freeing then from insecticide selection under harsh-rearing (Harsh lines) and optimal-rearing conditions (Optimal lines). The LD_<50> values were lower for the Harsh lines than for Optimal lines in all the generations. Egg size of both lines increased gradually through 10Generations. Comparisons of these two lines show that the egg size increased more rapidly in Harsh lines than in Optimal lines in early generations.Comparison of immature survivability of Harsh and Optimal lines reared under those conditions showed that the survivability of Harsh line individuals was significantly lower than for those of Optimal lines. These results suggest that susceptibility might recovery more quickly in Harsh lines than in Optimal lines because resistant insects with small eggs had lower susceptibility than susceptible insects with normal eggs. Less

建立了抗氰戊菊酯的小菜蛾品系，研究了亚致死剂量对小菜蛾卵大小和产卵量的影响。选择品系的卵显著小于未选择品系的卵。选择品系的雌虫繁殖力有高于非选择品系的趋势。为明确小菜蛾抗性发展和卵粒缩小的遗传方式，将其正反交F_1代与亲本进行了比较。还比较了正反交F1代以及抗、感品系间的适合度分量，以检测母体对蛋大小等适合度分量的影响。对感病雌虫与抗病雄虫（SR）和抗病雌虫与感病雄虫（RS）杂交F_1的剂量-死亡率回归线进行了拟合，结果表明： ...更多信息介于亲本系之间，即，SS和RR。这一结果表明，氰戊菊酯抗性的遗传方式既不是完全显性的，也不是完全隐性的，不存在性连锁。RR系的卵粒大小显著小于SS系。RS和SR系的卵子大小接近于它们自己的母系。认为抗性遗传方式与卵大小遗传方式不同，选择试验表明，这些参数之间存在负相关反应，但在严酷饲养条件下，通过10代的自由选择，检测了抗性品系对芬伐瑞酯的敏感性恢复情况（苛刻品系）和最佳饲养条件（最佳品系）。在<50>所有世代中，Harsh品系的LD_（？）值均低于Optimal品系。10代后，两品系的卵粒大小逐渐增大。结果表明，Harsh品系的早期卵粒增长速度快于Optimal品系，在此条件下饲养的Harsh品系和Optimal品系幼鱼存活率的比较表明，Harsh品系幼鱼存活率显著低于Optimal品系。这些结果表明，抗性在Harsh品系中比在Optimal品系中恢复得更快，因为具有小卵的抗性昆虫比具有正常卵的易感昆虫具有更低的易感性。少