Physiological role of IGF-I in the nutrition of central nervous system

IGF-I在中枢神经系统营养中的生理作用

• 批准号: 06454082
• 负责人: NOGUCHI Tadashi
• 金额: $ 4.74万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454082/
• 关键词: IGF-I; IGF-II; IGF-binding protein; Protein nutrition; Insulin-like growth factor; IGF・I; インスリン様成長因子-I; インスリン様成長因子結合タンパク質; 神経切断; インスリン様成長因子I; インスリン様成長因子Iレセプター; インスリン様成長因子I mRNA; インスリン様成長因子レセプターmRNA

To elucidate the physiological role of IGF-I in the nutrition of central nervous system, the following studies were performed.(1) Relate the effect of nutrition of the expressioin of IGF-I,IGF-II and IGF-binding protein genes to the mode of nutrient incorporation by the central nervous.(2) Elucidate the role of nervous system in the nutrition of tissues.Gene expression of IGF-I,IGF-II and IGF-binding prteins was studied in various portions of rat brain. IGF-I mRNA was found in various portions of brain but it was scarcely found in cerebrum and not so much in cerebellum and midbrain. On the other hand, IGF-II mRNA was found in every portion of brain. Among IGFBP-1 BP-2 and BP-3, only IGFBP-2 mRNA was found in brain extensively. Dictary protein did not affect the mRNA contents of IGF-I,IGF-II and IGFBP-2 in brain, probably reflecting the conservative nature of brain nutrition. It was suggested that brain nutrition is regulated not by endocrine mode of IGF-I action but by autocrine/paracrine mode of IGF-I action.As for the role of nervous system in the nutrition of tissues, protein metabolism of denervated muscle was studied in detail.

为了阐明IGF-I在中枢神经系统营养中的生理作用，进行了以下研究。(1)将营养对IGF-I、IGF-II和IGF-binding蛋白基因表达的影响与中枢神经系统的营养摄入方式联系起来。(2)为了阐明神经系统在组织营养中的作用，研究了大鼠脑不同部位IGF-Ⅰ、IGF-Ⅱ和IGF结合蛋白的基因表达。IGF-ImRNA在脑内分布广泛，但在大脑中几乎没有，在小脑和中脑中也不多见。另一方面，IGF-II mRNA在脑的各个部分都有表达。在IGFBP-1、BP-2和BP-3中，仅IGFBP-2 mRNA广泛分布于脑内。Dictary蛋白不影响脑内IGF-I、IGF-II和IGFBP-2 mRNA的含量，可能反映了脑营养的保守性。提示脑营养不是通过IGF-I作用的内分泌方式调节，而是通过IGF-I作用的自分泌/旁分泌方式调节。至于神经系统在组织营养中的作用，我们详细研究了去神经肌肉的蛋白质代谢。

项目成果

KIDA,S.,NOGUCHI,T.et al.: "Insulin-like growth factor-I messenger RNA content in the oviduct of Japanese quail(Coturnix coturnix japonica):changes during growth and development or after estrogen" Compt.Biochem.Physiol.109C. 191-204 (1994)

KIDA,S.,NOGUCHI,T.et al.：“日本鹌鹑（Coturnix coturnix japonica）输卵管中胰岛素样生长因子-I 信使 RNA 含量：生长发育期间或雌激素后的变化”Compt.Biochem.Physiol

TAKENAKA,A.: "Nutritional regulation of gene expression of insulin-like growth factor-binding profeins and the acid-labile subunit in various tissues of rats." J.Endocrinol.(in press). (1996)

TAKENAKA,A.：“大鼠各种组织中胰岛素样生长因子结合蛋白和酸不稳定亚基基因表达的营养调节。”

Asako Takenaka 他: "Effect of protein nutrition on insulin-like growth factor-I (IGF-I) receptor in various tissues of rats." J. Nutr. Sci. Vitaminol. submitted.

Asako Takenaka 等人：“蛋白质营养对大鼠各种组织中胰岛素样生长因子-I (IGF-I) 受体的影响。”J. Nutr Sci。

NOGUCHI,T.: "Gene expression of IGF-I and IGF-binding proteins." J.Reproduction and Development. (in press). (1996)

NOGUCHI,T.：“IGF-I 和 IGF 结合蛋白的基因表达。”

KIDA,S.,NOGUCHI,T.,et al.: "Effects of insulin-like growth factor-I,estrogen,glucocorticoid and transferrin on the mRNA contents of ovalbumin and conalbumin in primary cultures of quail(Coturnix coturnix japouica)" Compt.Biochem.Physiol.(印刷中). (1995)

KIDA, S., NOGUCHI, T., et al.：“胰岛素样生长因子-I、雌激素、糖皮质激素和转铁蛋白对鹌鹑（Coturnix coturnix japouica）原代培养物中卵清蛋白和伴清蛋白 mRNA 含量的影响”Compt .Biochem.Physiol.（出版中）（1995）。