Dietary control of membrane functions and qualities of cultured fish muscle

这项工作的目标是通过膳食油控制彩虹鳟鱼的骨架肌肉（SR）功能来调制彩虹鳟鱼，并控制质量，这样的新鲜性，由文化鱼肌肉组成。在1个月后，用必需脂肪酸（EFA）喂养两个月的测试后，用脂肪酸组合物和SRs进行了精确的饮食原因替代。每个麝香的磷酰胆碱片段和SR比喂养的饮食更容易改变的磷脂酰亚胺片段。Ca^<2+>-来自鱼类饲料n-3富饲料的ATP酶活性比来自n-6富饲料和不合格饲料组的SRs低。明显的ATP和肌酸磷酸盐（CP）降解是由n-6丰富和低氟醚饮食喂养缓慢地加速的。Muscles中乳酸菌的累积是由n-6丰富和全民教育不良的饮食喂养所记录的。如何， ATP n-3组的破坏率，是从CP退化率和乳酸累积率计算的，高于n-6组。这些结果认为SR膜的脂肪酸组成可以被饮食脂质控制，这样我们就可以控制生理SR功能，减少细胞钙^<2+>浓度，然后n-6喂养将减少细胞酸钙^<2+>集中和ATP破坏率。这就是为什么，提醒我们在休息的舞台上吃n-6种丰富的饮食，在1 - 2个月内就会做鱼麝香。由于这些饮食喂养将影响鱼的身体重量、能量消耗效率和其他信号转移机制，需要进一步的调查来评估n-6脂肪酸喂养对鱼麝香的影响。

The goals of this work are to modulate sarcoplasmic reticulum (SR) functions in rainbow trout skeltal muscle by dietary oils and to control qualities, such as freshness, of cultured fish muscle.・ Two-months feeding test after 1 month feeding with essential fatty acids (EFA) deficient diet cause alteration in fatty acid compositions of muscles and the SRs. Phosphatidylcholine fractions from each muscle and the SR were more easily altered by feeding diets than phosphatidylethanolamine fractions.・ Ca^<2+>-ATPase activities of the SR from fish fed n-3 rich diet were lower than those of the SRs from n-6 rich and EFA deficient groups.・ Apparent ATP and creatine phosphate (CP) degradations were slightly accelerated by n-6 rich and EFA deficient diet feedings. Accumulation of lactic acid in muscles was slightly retarded by n-6 rich and EFA deficient diet feedings. However, ATP breakdown rate of n-3 group, which was calculated from CP degradation and lactic acid accumulation rates, was higher than that of n-6 group.These results suggest that fatty acid compositions of SR membrane can be controled by dietary lipids, that we can control physiological SR functions, reducing cytosolic Ca^<2+> concentration, and that n-6 feeding would decrease cytosolic Ca^<2+> concentration and ATP breakdown rate. It is, therefore, reminescent to us that n-6 rich diet feeding for 1 or 2 months would make fish muscle in resting stage. However, because these diet feedings would influence fish body weight, energy consumption efficiency, and other signal transduction mechanisms, further investigations are required to evaluate effects of n-6 fatty acid feeding on fish muscle.