Dietary control of membrane functions and qualities of cultured fish muscle

养殖鱼肌肉膜功能和品质的饮食控制

• 批准号: 06454102
• 负责人: KOIZUMI Chiaki
• 金额: $ 4.42万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454102/
• 关键词: skeletal muscle; Ca^<2+>; n-6 polyunsaturated fatty acid; rainbow trout; sarcoplasmic reticulum; ATP; 品質保持; n-6系不飽和脂肪酸; n-3系不飽和脂肪酸; 筋細胞内Ca濃度; リン脂質

The goals of this work are to modulate sarcoplasmic reticulum (SR) functions in rainbow trout skeltal muscle by dietary oils and to control qualities, such as freshness, of cultured fish muscle.・ Two-months feeding test after 1 month feeding with essential fatty acids (EFA) deficient diet cause alteration in fatty acid compositions of muscles and the SRs. Phosphatidylcholine fractions from each muscle and the SR were more easily altered by feeding diets than phosphatidylethanolamine fractions.・ Ca^<2+>-ATPase activities of the SR from fish fed n-3 rich diet were lower than those of the SRs from n-6 rich and EFA deficient groups.・ Apparent ATP and creatine phosphate (CP) degradations were slightly accelerated by n-6 rich and EFA deficient diet feedings. Accumulation of lactic acid in muscles was slightly retarded by n-6 rich and EFA deficient diet feedings. However, ATP breakdown rate of n-3 group, which was calculated from CP degradation and lactic acid accumulation rates, was higher than that of n-6 group.These results suggest that fatty acid compositions of SR membrane can be controled by dietary lipids, that we can control physiological SR functions, reducing cytosolic Ca^<2+> concentration, and that n-6 feeding would decrease cytosolic Ca^<2+> concentration and ATP breakdown rate. It is, therefore, reminescent to us that n-6 rich diet feeding for 1 or 2 months would make fish muscle in resting stage. However, because these diet feedings would influence fish body weight, energy consumption efficiency, and other signal transduction mechanisms, further investigations are required to evaluate effects of n-6 fatty acid feeding on fish muscle.

这项工作的目标是通过食用油调节虹鳟鱼腹肌的肌浆网（SR）功能，并控制养殖鱼类肌肉的品质，如新鲜度。用必需脂肪酸（EFA）缺乏饲料喂养1个月后进行两个月的喂养试验，引起肌肉和SR脂肪酸组成的改变。来自每块肌肉和SR的磷脂酰胆碱组分比磷脂酰乙醇胺组分更容易通过喂食饮食改变。高n-3组SR的Ca^<2+>-ATPase活性低于高n-6组和EFA缺乏组。表观ATP和磷酸肌酸（CP）的降解略有加速n-6丰富和EFA缺乏饲料喂养。肌肉中乳酸的积累略有延迟n-6丰富和EFA缺乏的饮食喂养。而n-3组的ATP分解率（由CP降解率和乳酸积累率计算）高于n-6组，说明饲料脂类可以调控SR膜的脂肪酸组成，通过降低胞浆Ca^2+浓度，n-6饲喂会降低细胞质Ca^<2+>浓度和ATP分解率。因此，我们认为，投喂富含n-6的饲料1 ~ 2个月，会使鱼肌肉处于静止期。然而，由于这些饲料喂养会影响鱼的体重，能量消耗效率，和其他信号转导机制，需要进一步的研究，以评估n-6脂肪酸对鱼类肌肉的影响。