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Tumor necrosis factor receptor during acute Kawasaki disease

急性川崎病期间肿瘤坏死因子受体

基本信息

批准号：
06454301
负责人：
FURUKAWA Susumu
金额：
$ 2.94万
依托单位：
YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454301/
关键词：
Kawasaki disease Coronary artery lesion TNFalpha TNF receptor CD14_+ macrophage monocyte Electron microscope Nucleoli in monocyte Granules in cytoplasm マイクロビーズ

项目摘要

To evaluate the role of tumor necrosis factor alpha (TNF-alpha) during acute Kawasaki disease, we measured p60 soluble tumor necrosis factor receptor (sTNF-R) shedding into the circulation in 48 patients with acute Kawasaki disease, all of whom received intravenous infusions of gamma-globulin. Of the 48 patients, 5 had coronary artery lesions. Serum concentrations of p60 sTNF-R and TNF-alpha were measured by a sandwich enzyme immunoassay. Patients with Kawasaki disease had increased serum levels of p60 sTNF-R.We found a positive correlation between serum levels of p60 sTNF-R and levels of TNFalpha during acute Kawasaki disease. Moreover, patients with coronary artery lesions had higher levels of sTNF-R than did those without coronary artery lesions. Our findings indicate that p60 sTNF-R levels in serum may be useful for determining the severity of vascular damage during acute Kawasaki disease, and that patients with Kawasaki disease and high sTNF-R levels seem to be susceptible to coronary artery lesions even if they receive therapy with intravenous infusions of gamma-globulin.Next, we gathered peripheral blood CD14_+ monocytes through magnetic cell separator (MACS) and examined them using an elctron microscope in 5 patients with Kawasaki disease during the acute stage. MACS is a machine that can separate and select specific cells. A form that cannot be seen in normal monocytes was observed in patients with Kawasaki disease. Nucleoli were seen and the form of the nuclei was more complex and in addition, a large quantity of granules was seen in the cytoplasm during acute Kawasaki disease. It is our speculation that a relation may exist between cell activation such as the production of cytokines, in particular TNFalpha and those changes seen in the peripheral blood CD14_+ monocytes.

为了评价肿瘤坏死因子α（TNF-α）在急性川崎病中的作用，我们检测了48例急性川崎病患者的p60可溶性肿瘤坏死因子受体（sTNF-R），所有患者均接受了静脉输注丙种球蛋白。48例患者中，5例有冠状动脉病变。通过夹心酶免疫测定法测定p60 sTNF-R和TNF-α的血清浓度。川崎病患者血清p60 sTNF-R水平升高，急性期川崎病患者血清p60 sTNF-R水平与TNF α水平呈正相关。此外，冠状动脉病变患者的sTNF-R水平高于无冠状动脉病变的患者。我们的研究结果表明，血清中p60 sTNF-R水平可能有助于确定急性川崎病期间血管损伤的严重程度，并且患有川崎病和高sTNF-R水平的患者似乎对冠状动脉病变敏感，即使他们接受静脉输注丙种球蛋白的治疗。用磁性细胞分离器（MACS）分离5例急性期川崎病患者外周血CD 14_+单核细胞，并在电镜下观察其变化。MACS是一种可以分离和选择特定细胞的机器。在川崎患者中观察到一种在正常单核细胞中看不到的形式。急性期川崎可见核仁，细胞核形态较复杂，胞浆内可见大量颗粒。我们推测，细胞活化（例如细胞因子（特别是TNF α）的产生）与外周血CD 14+单核细胞中观察到的变化之间可能存在关系。