Molecular study of hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts
C3H10T1/2小鼠胚胎成纤维细胞造血支持能力的分子研究
基本信息
- 批准号:06454345
- 负责人:
- 金额:$ 3.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To investigate the molecular aspects of hematopoiesis-supporting ability of stromal cells, we used a differentiation-inducible mouse embryo fibroblast cell line, C3H10T1/2 (10T1/2). Stably determined preadipocyte and myoblast cell lines were established after a brief exposure of 10T1/2 cells to 5-azacytidine. These cell lines terminally differentiated into adipocytes and myotubes, respectively, under appropriate conditions. The hematopoiesis-supporting ability was significantly elevated at the preadipocyte stage (A54 preadipocyte), and was reduced after terminal adipocytic differentiation. To identify molecules that contribute to the hematopoiesis-supporting ability of preadipocytes, we screened genes that were differentially expressed in A54 preadipocytes and isolated the novel gene by mRNA differential display method. This gene was defined as a gene that was down-regulated during adipocyte differentiation-1 (drad-1). The drad-1 was expressed in other mouse preadipocytes, namely, ST2 and PA6 cells, that also have hematopoiesis-supporting ability. Moreover, the drad-1 was found to be expressed in mouse bone marrow. However, the function of the protein encoded by drad-1 is currently unknown. In addition, we investigated the change in CD34 mRNA expression during stromal cell differentiation. As a result, CD34 mRNA was constitutively expressed by parent 10T1/2 cells but not by adipogenically or myogenically determined cells. This finding supports the concept that CD34 may also be a marker of stromal progenitors and is lost as the cells differentiate into phenotypically distinct stromal elements. The 10T1/2-derived cell lines could provide a valuable tool to aid in the analysis of stromal cell development and differentiation and the search for novel stromal cell-derived factor (s).
为了研究基质细胞造血支持能力的分子机制,我们使用了诱导分化的小鼠胚胎成纤维细胞系C3H10T1/2 (10T1/2)。将10T1/2细胞短暂暴露于5-氮胞苷后,建立稳定的前脂肪细胞和成肌细胞系。在适当的条件下,这些细胞系最终分别分化为脂肪细胞和肌管。造血支持能力在前脂肪细胞阶段(A54前脂肪细胞)显著提高,在脂肪细胞终末分化后降低。为了确定有助于前脂肪细胞造血支持能力的分子,我们筛选了A54前脂肪细胞中差异表达的基因,并通过mRNA差异显示法分离了新基因。该基因被定义为在脂肪细胞分化过程中下调的基因-1 (drad-1)。drad-1在其他具有造血支持能力的小鼠前脂肪细胞,即ST2和PA6细胞中表达。此外,还发现drad-1在小鼠骨髓中表达。然而,由drad-1编码的蛋白质的功能目前尚不清楚。此外,我们还研究了间质细胞分化过程中CD34 mRNA表达的变化。结果,CD34 mRNA由亲本10T1/2细胞组成表达,而不是由脂肪形成或肌形成决定的细胞表达。这一发现支持了这样一种观点,即CD34也可能是基质祖细胞的标记物,并在细胞分化为表型不同的基质元素时丢失。10t1 /2衍生细胞系可以为分析基质细胞的发育和分化以及寻找新的基质细胞衍生因子提供有价值的工具。
项目成果
期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kobayashi, Y., Hayashi, Y., Ozawa, K., and Asano, S.: "HRX gene rearrangement in secondary acute lymphoblastic leukemia." Leukemia and Lymphoma. 17. 391-399 (1995)
Kobayashi, Y.、Hayashi, Y.、Ozawa, K. 和 Asano, S.:“继发性急性淋巴细胞白血病中的 HRX 基因重排。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hideki Suzuki: "A synergistic increase in transplantable peripheral blood cells in mice by co-administration of recombinant human IL-6 and recombinant hyman G-CSF." Transplantation. 59. 1596-1600 (1995)
Hideki Suzuki:“通过联合施用重组人 IL-6 和重组海曼 G-CSF,可协同增加小鼠可移植外周血细胞。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshihisa Tsuruta: "Effects of myeloid cell grcuth factors on alkaline phosphatase,myeloperxidase,defencin and G-CSFR mRNA expression in hematopoieticcells of normal individuals and myeloid" Brit.J.Haematol.(Cin press). (1996)
Toshihisa Tsuruta:“骨髓细胞生长因子对正常个体和骨髓造血细胞中碱性磷酸酶、髓过氧化物酶、防御素和 G-CSFR mRNA 表达的影响”Brit.J.Haematol.(Cin press)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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Takashi Yoshikubo: "Adhesion of NFS-60 myeloid leukemia cells to MC3T3-G2/PA6 stromal cells luduces granulocyte colony-stimulating factor production." Blood. 84. 415-420 (1994)
Takashi Yoshikubo:“NFS-60 髓系白血病细胞与 MC3T3-G2/PA6 基质细胞的粘附会诱导粒细胞集落刺激因子的产生。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hideki Suzuki: "A synergistic increase in transplantablc peripheral blood stem cdls in mice by co-administration of rhIL-6 and rhG-CSF" Transplantation. (in press). (1995)
Hideki Suzuki:“通过联合施用 rhIL-6 和 rhG-CSF 协同增加小鼠可移植性外周血干细胞的数量” 移植。
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- 影响因子:0
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OZAWA Keiya其他文献
OZAWA Keiya的其他文献
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{{ truncateString('OZAWA Keiya', 18)}}的其他基金
Development of a site-specific gene insertion technology for regenerative medicine:Basic study using developmental engineering
再生医学定点基因插入技术的开发:利用发育工程的基础研究
- 批准号:
23659493 - 财政年份:2011
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of gene therapy using bone-marrow-derived mesenchymal stem cells
使用骨髓间充质干细胞进行基因治疗的开发
- 批准号:
21390296 - 财政年份:2009
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of gene therapy for malignant lymphoma using mesenchymal stem cells with tumor-accumulating capacity
利用具有肿瘤蓄积能力的间充质干细胞开发恶性淋巴瘤基因治疗
- 批准号:
19390267 - 财政年份:2007
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of AAV (adeno-associated virus) vectors and their application to cancer therapy
AAV(腺相关病毒)载体的开发及其在癌症治疗中的应用
- 批准号:
17016067 - 财政年份:2005
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
DEDIFFERENTIATION OF NON-HEMATOPOIETIC TISSUE BY GENETIC MANIPULATION AND ITS ACQUISITION OF PLASTICITY AND HEMATOPOIETIC TRANSDIFFERENTIATION
通过基因操作实现非造血组织的去分化及其可塑性和造血转分化的获得
- 批准号:
16390281 - 财政年份:2004
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of the gene therapy technologies using adeno-associated virus (AAV)
使用腺相关病毒(AAV)的基因治疗技术的开发
- 批准号:
12470203 - 财政年份:2000
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development and application of the technologies for manipulationg hematopoietic stem cells using cell-regulatory genes
细胞调控基因操控造血干细胞技术的开发与应用
- 批准号:
11557075 - 财政年份:1999
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Development of the method for chromosomal site-specific integration of transgenes using AAV and its application to hematopoietic cells
AAV转基因染色体位点特异性整合方法的开发及其在造血细胞中的应用
- 批准号:
10470213 - 财政年份:1998
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a novel regulatory gene for in vivo & in vitro expansion of transduced hematopoietic stem cellss
开发一种新型体内调节基因
- 批准号:
09557087 - 财政年份:1997
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of a novel gene therapy technology for site-specific integration of large-sized genes
开发用于大尺寸基因位点特异性整合的新型基因治疗技术
- 批准号:
08457280 - 财政年份:1996
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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