Hormone secretion on several stages of tumorgenesity and growth rates of pituitary adenomas

垂体腺瘤肿瘤发生各阶段的激素分泌和生长速度

• 批准号: 06454415
• 负责人: SUMIDA Masayuki
• 金额: $ 0.58万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454415/
• 关键词: Prolactin; Reverse hemolytic plaque assay; Cell immunoblot assay; Autocrinologic growth; Cabergoline; Endocrinologic function; Cavernous sinus invasion; Growth rates; 下垂体腺腫; cell immunoblot assay; cavernous sinus invasion; type IV collagenase /

1. We studied prolactin (PRL) release from individual pituitary cells in normal and estrogen (E2) -treated female Fisher 344 rats by means of reverse hemolytic plaque assay. Multimodal distribution of PRL secretion was observed in normal pituitary cells. On the contrary, bimodal distribution was found in E2-treated pituitary cells. The heterogeneity of the PRL secretion from a single pituitary cell decreased in association with the length of E2 treatment. The PRL secretion of the E2-treated pituitary cells was less than that of the normal pituitary cells.2. Growth hormone-releasing hormone (GHRH) secreted by monodispersed human GH-producing pituitary adenoma cells was detected by the cell immunoblot assay. It is suggested that autocrinologic growth and/or local regulation of GH secretion by GHRH in human GH-producing pituitary adenomas.3. We examined an antitumoral effect of Cabergolinc (CG) , a dopamine agonist, with comparison to bromocriptine (BC) on E2-induced rat pituitary tumors … More In vitro and In vivo. The result suggests that CG has a higher affinity for the D2 receptor of pituitary cells and antitumor effect at a lower dosage than BC.4. On growth rates of human pituitary adenomas, we examined endocrinologic function, cavernous sinus invasion, and growth rates examined by using anti-proliferating cell nuclear antigen (PCNA) and MIB1 (a novel anti-Ki-67) as markers. The result suggest that endocrinologic function, cavernous sinus invasion, and growth rates are independent biologic behaviors each other.5. As a novel finding, on cavernous sinus invasion, we found that invasive adenomas had higher type IV collagenolytic activity than noninvasive adenomas. Thus, it is suggested that type IV collagenase activity in a pituitary adenoma may be related to its ability to invade the cavernous sinus invasion.6. Further examinations are needed on this points. However, we have already been started to examin matrix metalloproteinase-9, a type IV collagenase, secreted by human pituitary adenomas by means of the cell immunoblot assay. Less

1. 我们通过反向溶血斑测定法研究了正常和雌激素 (E2) 处理的雌性 Fisher 344 大鼠个体垂体细胞中催乳素 (PRL) 的释放。在正常垂体细胞中观察到 PRL 分泌的多峰分布。相反，在 E2 处理的垂体细胞中发现双峰分布。单个垂体细胞分泌 PRL 的异质性随着 E2 治疗时间的延长而降低。 E2处理后的垂体细胞PRL分泌量低于正常垂体细胞。 2．通过细胞免疫印迹法检测单分散的人产生 GH 的垂体腺瘤细胞分泌的生长激素释放激素（GHRH）。这表明人产生GH的垂体腺瘤存在自分泌生长和/或GHRH对GH分泌的局部调节。3．我们在体外和体内研究了多巴胺激动剂卡麦角林 (CG) 的抗肿瘤作用，并与溴隐亭 (BC) 对 E2 诱导的大鼠垂体瘤进行比较。结果表明，CG与垂体细胞D2受体的亲和力比BC.4更高，并且在较低剂量下具有抗肿瘤作用。关于人垂体腺瘤的生长速度，我们检查了内分泌功能、海绵窦侵犯以及使用抗增殖细胞核抗原（PCNA）和MIB1（一种新型抗Ki-67）作为标记物检查的生长速度。结果表明，内分泌功能、海绵窦浸润和生长速度是相互独立的生物学行为。5．作为一项新发现，关于海绵窦侵袭，我们发现侵袭性腺瘤比非侵袭性腺瘤具有更高的 IV 型胶原溶解活性。因此，提示垂体腺瘤中IV型胶原酶活性可能与其侵袭海绵窦的能力有关。6．这一点还需要进一步检查。然而，我们已经开始通过细胞免疫印迹测定来检查基质金属蛋白酶-9，这是一种 IV 型胶原酶，由人垂体腺瘤分泌。较少的

项目成果

Hitoshi Kawamoto, et al.: "Analysis of the growth rate and cavernous sinus invasion of pituitary adenomas" Acta Neurochirurgica. 136. 37-43 (1995)

Hitoshi Kawamoto 等：“垂体腺瘤的生长速度和海绵窦侵袭分析”Acta Neurochirurgica。

Masami Yoshikawa,et al.: "Characteristics of prolactin secretion in normal and estrogen-treated pituitaries of rats at the single cell level:analysis by reverse hemolytic plaque assay" Endocrine Journal. 42(2). 235-243 (1995)

Masami Yoshikawa 等人：“单细胞水平上正常和雌激素处理的大鼠垂体中催乳素分泌的特征：通过反向溶血斑测定进行分析”内分泌杂志。

Hitoshi Kawamoto,et al.: "Type IV collagenase activity and cavernous sinus invasion in human pituitary adenomas" Acta Neurochirurgica. 138(in press). (1996)

Hitoshi Kawamoto 等人：“人类垂体腺瘤中的 IV 型胶原酶活性和海绵窦侵袭”Acta Neurochirurgica。

Kuniki Eguchi, et al.: "Effect of cabergoline, a dopamine abonist, on estrogeninduced rat pituitary tumors : In vitro culture studies" Endocrine Journal. 42(3). 413-420 (1995)

Kuniki Eguchi 等人：“多巴胺无元剂卡麦角林对雌激素诱导的大鼠垂体瘤的影响：体外培养研究”内分泌杂志。

Masami Yoshikawa,Tohru Uozumi,Kciichi Kawamoto,et al.: "Characteristics of prolactin secretion in normal and estrogen-treated pituitaries of rats at the single cell level:analysis by reverse hemolytic plaque assay" Endocrine Journal. 42(in press). (1995)

Masami Yoshikawa、Tohru Uozumi、Kciichi Kawamoto 等人：“单细胞水平上正常和雌激素处理的大鼠垂体中催乳素分泌的特征：通过反向溶血斑测定进行分析”内分泌杂志。