Studies on conservation and genetic diversity in an endangered frog species Odorrana ishikawae

濒危青蛙石川蛙的保护和遗传多样性研究

• 批准号: 20510216
• 负责人: SUMIDA Masayuki
• 金额: $ 3.08万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20510216/
• 关键词: 生物多様性保全; 絶滅危惧種; 飼育下繁殖; 遺伝的多様性; 交雑実験; 種分化; イシカワガエル; 新種; マイクロサテライト; ミトコンドリアDNA; リュウキュウアカガエル; 人工繁殖; 交配後隔離; 雑種致死; 精子形成異常; アロザイム

Odorrana ishikawae is listed as a class BI endangered species on IUCN Red List and protected by law in Okinawa and Kagoshima Prefectures, Japan. In an effort to help preserve this endangered species, we tested a farming technique involving the artificial breeding of frogs and also promoted natural breeding in the laboratory. We also conducted morphological comparison, mtDNA analysis, and crossing experiment, with the purpose of clarifying the degree of differentiation between the Okinawa and Amami populations. The results showed that the Okinawa and Amami populations are clearly differentiated both genetically and morphologically, and also reproductively isolated by complete hybrid inviability or abnormal spermatogenesis. These two populations can be regarded as different species. Thus, we have described the Amami population as a new species O. splendida.

石川臭蛙是世界自然保护联盟红色名录中的BI类濒危物种，在日本的冲绳县和鹿儿岛县受到法律保护。为了保护这种濒危物种，我们测试了一种涉及青蛙人工繁殖的养殖技术，并在实验室中促进了自然繁殖。此外，我们还进行了形态学比较、mtDNA分析和杂交试验，以阐明冲绳和奄美种群之间的分化程度。结果表明，冲绳和奄美群体在遗传和形态上都存在明显的分化，并且由于完全的杂种不育或精子发生异常而在生殖上隔离。这两个种群可以被视为不同的物种。因此，我们将奄美居群描述为一新种O.太棒了

项目成果

Complete mitochondrial genomes of three neobatrachian anurans: A case study of divergence time estimation using different data and calibration settings

• DOI: 10.1016/j.gene.2007.10.001
• 发表时间: 2008-01-15
• 期刊: GENE
• 影响因子: 3.5
• 作者: Igawa, Takeshi;Kurabayashi, Atsushi;Sumida, Masayuki
• 通讯作者: Sumida, Masayuki

Mitochondrial DNA diversification, molecular phylogeny, and biogeography of the primitive rhacophorid genus Buergeria in East Asia

东亚原始石藻属 Buergeria 的线粒体 DNA 多样性、分子系统发育和生物地理学

• 发表时间: 2011
• 期刊: Molecular Phylogenetics and Evolution
• 影响因子: 4.1
• 作者: Nishizawa;T.;A.Kurabayashi;T.Kunihara;N.Sano;T.Fujii.;M.Sumida
• 通讯作者: M.Sumida

Development of microsatellite markers and population genetic analysis of the endangered frog, Odorrana ishikawae (Anura : Ranidae)

濒危青蛙 Odorrana ishikawae（无尾蛙：蛙科）的微卫星标记开发和群体遗传分析

• 发表时间: 2010
• 作者: Igawa T.;M.Okuda;S.Oumi;S.Katsuren;A.Kurabayashi;T.Umino;M.Sumida
• 通讯作者: M.Sumida

沖縄産と奄美産の準絶滅危惧種リュウキュウアカガエルにおける遺伝的分化

冲绳和奄美近危琉球红蛙的遗传分化

• 发表时间: 2009
• 作者: 岩成美紀;藤井保;井川武;Islam;M. M.;住田正幸;大海昌平;勝連盛輝
• 通讯作者: 勝連盛輝

Attempts at artificial breeding and analyses of genetic diversity in Odorrana ishikawae, an endangered frog species : Case study of fauna conservation from Japan

濒临灭绝的青蛙 Odorrana ishikawae 的人工繁殖尝试和遗传多样性分析：日本动物群保护案例研究

• 发表时间: 2009
• 作者: 住田正幸;佐藤直樹;倉林敦;大海昌平;勝連盛輝;太田英利;福庭博子;菅原芳明;藤井保;住田正幸
• 通讯作者: 住田正幸