Molecular evolutionary study on the amphibian mitochondrial genomes

两栖动物线粒体基因组的分子进化研究

• 批准号: 13839012
• 负责人: SUMIDA Masayuki
• 金额: $ 2.37万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13839012/
• 关键词: mitochondrial DNA genome; complete nucleotide sequence; gene arrangement; molecular evolution; Rana nigromaculara; Buergeria buergeri; amphibians; 両生類; ヒキガエル類; 種分化; 系統進化

In this study we determined the complete nucleotide sequences of the mitochondrial DNA of the ranid frog Rana nigromaculata and the rhacophorid frog Buergeria buergeri. The gene content, nucleotide composition, and colon usage of these frogs conformed to those of typical vertebrate patterns. However, due to an accumulation of lengthy repetitive sequences into the D-loop region, these species possess the large mitochondrial genomes among all the vertebrates examined so far (Rana nigromaculata, 17,804 bp ; Buergeria buergeri, 19,959 bp). In a comparison of gene organizations among amphibian species (Xenopus, salamanders and a caecilian), the positioning of four tRNA genes and the ND5 gene in the mtDNA of B. buergeri diverged from the common vertebrate gene arrangement shared in Xenopus, salamander and caecilian. The unique positions of the tRNA genes in B. buergeri are shared by ranid frogs, indicating that the rearrangements of the tRNA genes occurred in a common ancestral lineage of ranids and rhacophorids. On the other hand, the novel position of the ND5 gene seemed to have arisen in a lineage leading to rhacophorids (and other closely related taxa) after ranid divergence. Phylogenetic analysis based on nucleotide sequence data of all mitochondrial genes also supported the gene rearrangement pathway

在这项研究中，我们测定了黑斑蛙和布氏蛙线粒体DNA的完整核苷酸序列。这些青蛙的基因含量、核苷酸组成和结肠使用符合典型的脊椎动物模式。然而，由于在d环区域积累了冗长的重复序列，这些物种在迄今为止所研究的所有脊椎动物中拥有较大的线粒体基因组（Rana nigromaculata, 17,804 bp; Buergeria buergeri, 19,959 bp）。在两栖动物物种（爪蟾、蝾螈和蛭形动物）的基因组织比较中，布氏B. buergeri mtDNA中4个tRNA基因和ND5基因的定位与爪蟾、蝾螈和蛭形动物共有的脊椎动物基因排列不同。该tRNA基因的独特位置在蛙类蛙类中是共享的，这表明tRNA基因的重排发生在蛙类和蛙类的共同祖先谱系中。另一方面，ND5基因的新位置似乎出现在ranid分化后导致racophorids（和其他密切相关的分类群）的谱系中。基于所有线粒体基因核苷酸序列数据的系统发育分析也支持基因重排途径

项目成果

Sumida, Masayuki: "Complete nucleotide sequence and gene rearrangement of the mitochondrial genome of the Japanese pond frog Rana nigromaculata"Genes & Genetic Systems. 76(5). 311-325 (2001)

Sumida，Masayuki：“日本池塘蛙黑斑蛙线粒体基因组的完整核苷酸序列和基因重排”基因

Sumida, Masayuki: "Reproductive isolating mechanisms and molecular phylogenetic relationships among brown frogs distributed in the Palearctic region"Zoological Science. 20(5). 567-580 (2003)

Sumida，Masayuki：“古北区褐蛙的生殖隔离机制和分子系统发育关系”动物学。

Sumida, Masayuki: "Inter- and intraspecific evolutionary relationships of the rice frog Rana limnocharis and the allied species R. cancrivora inferred from crossing experiments and mitochondrial DNA sequences of the 12S and 16S rRNA genes"Molecular Phylog

Sumida，Masayuki：“根据杂交实验和 12S 和 16S rRNA 基因的线粒体 DNA 序列推断出稻蛙 Rana limnocharis 和近缘物种 R. cancrivora 的种间和种内进化关系”Molecular Phylog

住田正幸: "カエルの遺伝研究最先端(松井正文編)"「これからの両棲類学」裳華房(印刷中). (2004)

住田雅之：“青蛙的尖端遗传研究（松井雅文编辑）”“未来两栖生物学”Shokabo（正在出版）（2004年）。

Sumida, Masayuki: "Sequencing and analysis of the internal transcribed spacers (ITSs) and coding regions in the EcoR I fragment of the ribosomal DNA of the Japanese pond frog Rana nigromaculata"Genes & Genetic Systems. 79(2)(In press). (2004)

Sumida, Masayuki：“日本池塘蛙黑斑蛙核糖体 DNA 的 EcoR I 片段中的内转录间隔区 (ITS) 和编码区的测序和分析”基因