Clinical Significance of Platelet Allo-antibodies.

1)我们无法检测到Mo^a，Sr^a或Va^a抗原在日本人使用我们的血小板面板。2）我们经历了29例NAIT（新生儿同种免疫性血小板减少症）：20例由于抗HPA-4 B（Yuk^a）抗体，3例由于抗HPA-4 a（Yuk^B），5例由于抗HPA-3 a（巴克^a），1例由于抗HPA-5 B（Br^a）。3）通过对大量血小板输注无效患者的筛查，发现抗HPA-2b（Sib^a）在血小板输注无效患者中起重要作用; 4）MPHA（混合被动血凝）法是15年前发展起来的一种简便、敏感的抗血小板抗体检测方法。现在已经完善了。使用5%蔗糖，血小板固定板可以保持冷冻（低于-40 ℃）而不丧失反应性超过一年。因此，MPHA应作为医院实验室检测血小板同种抗体的常规试验。

1) We were unable to detect Mo^a, Sr^a or Va^a antigens among Japanese using our platelet-panel. In contrast, about 5% (15/314) were found to be Tu^a positive.2) We have experienced 29 cases of NAIT (Neonatal Alloimmune Thrombocytopenia) : 20 cases due to anti-HPA-4b (YuK^a) antibodies, 3 cases due to anti-HPA-4a (Yuk^b), 5 cases due to anti-HPA-3a (Bak^a) and 1 case due to anti-HPA-5b (Br^a). Among them 3 cases were complicated with intracranial hemorrhage.3) By screening a large number of samples, we found that anti-HPA-2b (Sib^a) playd an important role in the case of platelet transfusion refractoriness.4) The MPHA (mixed passive hemagglutination) method was developed about 15 years ago as a simple and sensitive test for the detection of anti-platelet antibodies. It has been refined now. Using 5% sucrose, the platelet-fixed plate can be kept frozen (below-40 C) without loss of reactivities for more than a year. Thus, MPHA should be employed as the routine test in any lab in the hospital for the detection of platelet allo-antibodies.