Development of MPHA kit for detecting antiplatelet antibodies.

开发用于检测抗血小板抗体的MPHA试剂盒。

• 批准号: 08557149
• 负责人: SHIBATA Yoichi
• 金额: $ 13.18万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557149/
• 关键词: Platelet Allo-antigen; Platelet Allo-antibody; Platelet Transfusion; Neonatal Alloimmune Thrombocytopenia; 血小板輸血不応答; 新生児血小板減少性紫斑病; 血小板抗原; HPA; MPHA

Platelet alloantigens are known to be involved in neonatal alloimmune thrombo-cytopenia (NAIT), post-transfusion purpura (PTP) and platelet transfusion refractoriness (PTR). Thus far there has been no simple and sensitive method for detecting the antibodies to these platelet alloantigens.Using MPHA (mixed passive hemagglutination), we (Japanese platelet serology reference laboratory) analyzed NAIT cases among Japanese and confirmed the presence of the platelet antibodies in 40 cases.Seventeen years ago, we developed MPHA method for detecting antiplatelet antibodies. However it was difficult to keep platelet antigens for a long time, a significant fault making the method inappropriate for routine use.We have improved the method ; soluble fraction extracted from platelet concentrate was used for platelet antigens.Thses improvements allow us to use the MPHA kit for routine screening. At present, the MPHA kit can detect the following antibodies : anti-HPA-la, -2a, -2b, -3a, -3b, -4a, -4b, -5a, -5b, -6b, and anti-Nak^a antibodies. Among them, anti-HPA-2b, -3a, -4b, -5b, -6b and anti-Nak^a antibodies are particularly important among Japanese.

已知血小板同种抗原与新生儿同种免疫性血小板减少症（NAIT）、输血后紫癜（PTP）和血小板输注无效（PTR）有关。迄今为止，还没有简单而敏感的方法来检测这些血小板同种抗原的抗体。我们（日本血小板血清学参考实验室）使用MPHA（混合被动血凝）分析了日本人中的NAIT病例，并确认了40例患者中存在血小板抗体。17年前，我们开发了MPHA方法来检测抗血小板抗体。但由于该方法不易长期保存血小板抗原，不适合常规应用，我们对该方法进行了改进，从血小板浓缩液中提取可溶性部分用于血小板抗原，使MPHA试剂盒可用于常规筛查。目前，MPHA试剂盒可检测以下抗体：抗HPA-1a、-2a、-2b、-3a、-3b、-4a、-4b、-5a、-5b、-6b和抗Nak α抗体。其中，抗HPA-2b、-3a、-4b、-5b、-6b和抗Nak ^a抗体在日本人中尤为重要。

项目成果

Fujiwara, K.: "Large-scale DNA typing for human platelet alloantigens by PCR-PHFA(preferential homoduplex formation assay)" British Journal of Haematology. 95. 198-203 (1996)

Fujiwara, K.：“通过 PCR-PHFA（优先同源双链体形成测定）对人血小板同种抗原进行大规模 DNA 分型”英国血液学杂志。

Takeuchi, F, Kuwata, S, Naβ, H, Hong G.H, Shibata, Y, Tanimoto, K, Ito, K: "Association of TAP1 and TAP2 with systemic sclerosis in Japanese" Clinical and Experimental Rheumatology.14. 513-521 (1996)

Takeuchi, F, Kuwata, S, Naβ, H, Hong G.H, Shibata, Y, Tanimoto, K, Ito, K：“TAP1 和 TAP2 与日本系统性硬化症的关联”临床和实验风湿病学 14。 1996）

Maejima M., Fujii T., Kozuma S., Okai T., Shibata Y.and Taketani Y.: "Presence of HLA-G-Expressing Cells Modulates the Ability of Peripheral Blood Mononuclear Cells to Release Cytokines American Journal of Reproductive" Immunology. 38. 79-82 (1997)

Maejima M.、Fujii T.、Kozuma S.、Okai T.、Shibata Y. 和 Taketani Y.：“HLA-G 表达细胞的存在调节外周血单核细胞释放细胞因子的能力 美国生殖杂志”免疫学

Wang, H, Tokunaga, K, Akaza, T, Tadokoro, K, Sibata, Y, Juji, T.: "Identification of HLA-C alleles using PCR-Single-strand-conformation polymorphism and direct sequeno.ing" Tissue Antigens.49. 134-140 (1997)

Wang, H, Tokunaga, K, Akaza, T, Tadokoro, K, Sibata, Y, Juji, T.：“使用 PCR 单链构象多态性和直接测序鉴定 HLA-C 等位基因”组织抗原。

Wang H.: "A novel allele,Cw^*1702 with serological Cw2 specificity" Tissue Antigens. 49. 183-185 (1997)

Wang H.：“一种新颖的等位基因，Cw^*1702，具有血清学 Cw2 特异性”组织抗原。