Screening of anti-platelet antibodies and prospective study of neonatal alloimmune thrombocytopenia

抗血小板抗体的筛选及新生儿同种免疫性血小板减少症的前瞻性研究

• 批准号: 10470515
• 负责人: SHIBATA Yoichi
• 金额: $ 8万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470515/
• 关键词: Pletelet Allo-antigen; Platelet Allo-antibody; Platelet Transfusion; Neonatal Alloimmune Thrombocytopenia; 血小板(HPA); 新生児血小板減少症紫斑病; 血小板輸血不応答

Platelet alloantibodies play a important role in neonatal alloimmune thrombocytopeniaHPA (human platelet antigens) are known to be involved in neonatal allo-immunethrombocytopenia (NAIT). For the detection of platelet antibodies, we developed MPHA (mixed passive hemagglutination) method for detecting anti-platelet antibodies.Using MPHA test, we screened 20,333 sera from pregnant women (28-30 weeks gestation) and obtained the following results.(1) Unexpectedly high prevalence of platelet antibodies in 198 sera (0.97%) The specificity of them were as follows,anti-HPA-5b 168 sera(82, 3%), anti-HPA-4b 27 sera(13, 6%)anti-HPA-6b 2 sera(1, 0%), anti-Nak-a 5 sera(2, 5%)anti-HPA-4b+anti-HPA-5b 1 serum(0, 5%).(2) Five cases of NAIT were due to anti-HPA-5b, two cases of NAIT were due to anti-HPA-4b, and one case was due to anti-HPA-4b and anti-HPA-5b. This is the first prospective study on neonatal alloimmune thrombocytemia.

血小板同种抗体在新生儿同种免疫性血小板减少症中起重要作用。HPA（人类血小板抗原）参与新生儿同种免疫性血小板减少症（NAIT）。为了检测血小板抗体，我们建立了检测抗血小板抗体的混合被动血凝（MPHA）法，用MPHA法对20，333份孕妇（孕28-30周）血清进行了筛查，结果如下：(1)198份血清中血小板抗体检出率为0.97%，其特异性依次为：抗HPA-5 b 168份（82.3%），抗HPA-4 b 27份（13.6%），抗HPA-6 b 2份（1.0%），抗Nak-α 5份（2.5%），抗HPA-4 b+抗HPA-5 b 1份（0.5%）。(2)5例NAIT是由于抗HPA-5 b，2例NAIT是由于抗HPA-4 b，1例是由于抗HPA-4 b和抗HPA-5 b。这是第一个关于新生儿同种免疫性血小板血症的前瞻性研究。

项目成果

Saeki H.: "Analysis of HLA Class II and TPA Alleles in Japanese Patients with Psoriasis Vulgaris"Human Immunology. 59. 503-511 (1998)

Saeki H.：“日本寻常型银屑病患者的 HLA II 类和 TPA 等位基因分析”人类免疫学。

Saeki H.: "Polymorphisms of HLA-DM genes in Japanese patients with psoriasis vulgaris"British Journal of Dematology. 141. 113-115 (1999)

Saeki H.：“日本寻常型银屑病患者 HLA-DM 基因的多态性”英国皮肤病学杂志。

Fujiwara K, Watanabe Y, Mitsunaga S, Oka T, Yamane A, Akaza T, Tadokoro K, Tokunaga K, Shibata Y, Juji T.: "Determination of Granulocyte-Specific Antigens on Neotrophil Fc γ Recepter IIIb by PCR-preferential Homoduplex Formation Assay, and Gene Frequencie

Fujiwara K、Watanabe Y、Mitsunaga S、Oka T、Yamane A、Akaza T、Tadokoro K、Tokunaga K、Shibata Y、Juji T.：“通过 PCR 优先同源双链体形成测定新生粒细胞 Fc γ 受体 IIIb 上的粒细胞特异性抗原测定和基因频率

Maejima M, Fujii T, Yamashita T, Hara N, Hamai Y, Miki A, Kozuma S, Okai T, Shibata Y, Taketani Y.: "Immunotherapy Before and During Pregnancy Improves Pregnancy Outcome in Women Who Suffer from Recurrent Abortion and Did Not Benefit from Immunothrapy Bef

Maejima M、Fujii T、Yamashita T、Hara N、Hamai Y、Miki A、Kozuma S、Okai T、Shibata Y、Taketani Y.：“怀孕前和怀孕期间的免疫治疗可改善患有复发性流产的女性的怀孕结果

Saeki H: "Polymorphisms of HLA-DM genes in Japanese Patients with psoriasis vulgaris"British Journal of Dematology. 141. 113-115 (1999)

Saeki H：“日本寻常型银屑病患者 HLA-DM 基因的多态性”英国皮肤病学杂志。