Molecular architecture and construction of the egg envelope in fish

鱼卵包膜的分子结构和构建

• 批准号: 06454688
• 负责人: YAMAGAMI Kenjiro
• 金额: $ 4.54万
• 依托单位: Faculty of Science and Technology, Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454688/
• 关键词: Egg envelope (Egg membrane); Fish egg; Egg envelope subunit; SF substances; Hatching enzyme; Chorion hardening; 卵膜タンパク質

The results of the research performed under the title described above are summarized as follows :(1) To analyze the structure of the egg envelope subunits by clarifying the structure of the precursor proteins of the subunits, we have cloned and analyzed the cDNAs for the precursors of Z1-1, 2 and Z1-3, H-SF and L-SF.Thus the primary structures of these precursors are almost clarified. (2) By directly analyzing the structure of the egg envelope subunits, some C-terminal peptides of the precursors (at least L-SF) were found to be cleaved off before the precursor proteins are assembled to form the egg envelope. Cross-linking of gamma-Glu-epsilon-Lys occurs between the assembled subunits to form a hardened egg envelope. This change was found to be catalyzed by transglutaminase associating with the egg envelope. (3) HCE liberated some peptides from the Z1-1, 2 subunits, which contained a high concentration of Pro and gamma-Glu-epsilon-Lys crosslinks. These peptide regions seemed to be responsible for construction of the ahrdened egg envelope. The present results have provided us with a lot of invaluable information of molecular structure and construction of the fish egg envelope.

（1）为了研究卵被膜亚基的结构，阐明其前体蛋白的结构，我们克隆了Z1-1、Z1 - 2和Z1-3前体蛋白、H-SF和L-SF的cDNA，并对其进行了分析，基本上阐明了这些前体蛋白的一级结构。(2)通过直接分析卵被膜亚基的结构，发现前体蛋白（至少是L-SF）的一些C端肽在前体蛋白组装形成卵被膜之前被切割掉。γ-Glu-ε-Lys的交联发生在组装的亚基之间以形成硬化的卵包膜。这种变化被发现是由与鸡蛋包膜结合的转氨酶催化的。(3)HCE从Z1-1，2亚基释放出一些肽，其含有高浓度的Pro和γ-Glu-ε-Lys交联。这些肽区似乎负责构建去角质的卵膜。本研究结果为了解鱼卵被膜的分子结构和结构提供了重要的信息。

项目成果

Sugiyama, H., K.Murata, I.Iuchi & K.Yamagami: "Evaluation of solubilizing methods of the egg envelpe of the fish, Oryzias latipes, and partial determanation of amino acid sequence of its subunit protein, ZI-3." Comp.Biochem.Physiol.(B). (in press). (1996)

杉山 H.、村田 K.、I.Iuchi

Yamagami, K., K.Murata, T.S.Hamazaki & I.Iuchi: In Perspectives in Comparative Endocrinology. Intrahepatic formation of SF-substances, the precursors of egg envelpe proteins, in response to estrogen administration in fish., 316-324 (1994)

山上 K.、村田 K.、滨崎 T.S.

Murata,K.et al: "Synchronous Production of the low-and high-molecular-weight precursors of the egg envelope sulunits,in response to estroqeu administration in the teleost fish,Oryzias latipes" Gen,Comp,Endocrinol.95. 232-239 (1994)

Murata,K.等人：“卵膜 sulunits 低分子量和高分子量前体的同步生产，响应硬骨鱼 Oryzias latipes 中的 estroqeu 给药”Gen,Comp,Endocrinol.95。

Yamagami K.et al.: "Perspectives in Comparative Endocrinology" Nat.Res.Council,Canada, 654 (1994)

Yamagami K.等人：“比较内分泌学的观点”Nat.Res.Council，加拿大，654 (1994)

Iuchi, I., C-R.Ha, H.Sugiyama & K.Nomura: "Analysis of chorion hardening of eggs of rainbow trout, Oncorhynchus mykiss." Dev.Growth Diff.(in press ). (1996)

Iuchi, I.、C-R.Ha、H.Sugiyama