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Development of an on-line sensing system of catecholamines and its application for medical use

儿茶酚胺在线传感系统的研制及其医疗应用

基本信息

批准号：
06454723
负责人：
MABUCHI Kimihiko
金额：
$ 4.22万
依托单位：
Research Center for Advanced Science and Technology, The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

An electrochemical sensor system was developed which allows real-time measurement and feedback of catecholamine concentrations, and which can be used in the control of artificial hearts.Electrochemical analyzes were carried out using a carbon fiber working electrode, an Ag-AgCl reference electrode, and a potentiostat. The operating parameters of the pneumatically-driven artificial heart system were altered in accordance with the algorithm for changes in the catecholamine concentration.The minimum detectable concentrations of both adrenaline and noradrenaline in a mock circulatory system using a phosphate-buffered solution were approximately 1-2 ng/ml (10^<-8> mol/l). An artificial heart control system utilizing this arrangement performed satisfactorily without delay although the sensitivity of the sensor decreased when placed in goat plasma instead of phosphate-buffered solution, due to the adsorption on to the electrodes of various substances such as plasma proteins.A microdialysis te … More chnique was therefore used in order to prevent blood proteins from being adsorbed on to the electrods. The dialytic solution was flushed through a cellulose microtube (cut-off molecular weight=50000) for dialysis ; the microtube was immersed in the goat plasma, and the catecholamine concentrations in the dialytic solution were measured using the above electrochemical detector. With this system, the ratio between catecholamine concentrations in the dialytic solution and that in the plasma was approximately 20% when the flow rate of the dialytic solution was 10 ml/min, with a time delay of approximately 90 seconds. Using this system, the minimum detectable concentration of both plasma adrenaline and noradrenaline was 10^<-7> mol/l ; it was possible to alter the driving conditions in accordance with the algorithm for changes in the catecholamine concentration of the plasma. The authors are currently attempting to : 1)incorporate the electrodes and the dialytic system into one microsensor system, and 2)improve the sensitivity of the sensor to 10^<-9> mol/l. Less

开发了一种电化学传感器系统，可以实时测量和反馈儿茶酚胺的浓度，并可用于人工心脏的控制。采用碳纤维工作电极、Ag-AgCl参比电极和恒电位器进行电化学分析。根据儿茶酚胺浓度变化的算法改变气动驱动人工心脏系统的运行参数。在使用磷酸盐缓冲溶液的模拟循环系统中，肾上腺素和去甲肾上腺素的最低检测浓度约为1-2 ng/ml （10^<-8> mol/l）。利用这种安排的人工心脏控制系统表现令人满意，没有延迟，尽管传感器的灵敏度下降，当放置在山羊血浆而不是磷酸盐缓冲溶液，由于吸附在电极上的各种物质，如血浆蛋白。因此，为了防止血液蛋白被吸附到电极上，使用了更多的技术。透析液通过纤维素微管（截止分子量=50000）冲洗透析；将微管浸入山羊血浆中，利用上述电化学检测器测定透析液中的儿茶酚胺浓度。当透析液流速为10 ml/min时，透析液中儿茶酚胺浓度与血浆中儿茶酚胺浓度之比约为20%，时间延迟约为90秒。使用该系统，血浆肾上腺素和去甲肾上腺素的最低检测浓度均为10^<-7> mol/l；根据血浆中儿茶酚胺浓度的变化，可以根据算法改变驾驶条件。作者目前正在尝试：1)将电极和透析系统整合到一个微传感器系统中，2)将传感器的灵敏度提高到10^<-9> mol/l。少