Visual information transmission in cat retinal ganglion cells with regenerated axons through the peripheral nerve graft.

在麻醉下完全切除成年猫的视神经，移植自体坐骨神经。通过记录再生轴突视网膜神经节细胞的单单位活动和记录模式反转的ergg来研究再生轴突视网膜神经节细胞的视觉功能。结果总结如下。(1)存活约60 d时，再次麻醉和固定。将移植物神经解剖成细束，用银丝电极记录单个神经活动。共记录286个视觉反应单元，并根据感受野性质将其分为以下组：142个为Y细胞，113个为X细胞，19个为W细胞，其余12个未分类。除中央X细胞外，大部分细胞的感受野中心大小正常。ON和Off中心细胞的出现明显不对称，特别是在X细胞中，没有Off中心细胞，而有很多ON中心细胞。(2)视神经切断术和神经移植手术相同后，对猫进行再麻醉，记录亮度正弦调制的垂直光栅对电图的响应。术后2 ~ 8周，模式逆转的ergg振幅逐渐降低，但与视神经切除的猫相比，这种下降是轻微的，更渐进的，这表明神经移植物对axoectomy的神经节细胞逆行性死亡有一定的挽救作用。

Under anesthesia the optic nerve of adult cats was completely sectioned and an autologous sciatic nerve was grafted. Visual functions of the retinal ganglion cells with regenerated axons were studied first by recording single unit activities from regenerated axons and secondly by recording pattern reversed ERGs. Results are summarized below. (1) At about 60 days survival the animals were reanesthetized and immobilized. The grafted nerve was dissected into fine fascicles and single unit activities were recorded with silver wire electrodes. A total of 286 visually responsive units were recorded and they were classified into the following groups based on receptive field properties : 142 were Y cells, 113 were X cells, 19 were W cells and the rest 12 were unclassified. Receptive field center size was normal in most units except for central X cells with enlarged fields. There was a clear asymmetry in the occurrence of ON and Off center cells especially among X cells where there was no OFF center cells while many ON center cells were recorded. (2) After the same surgery of optic nerve section and nerve graft, cats were reanesthetized and ERGs were recorded in response to vertical gratings whose luminance was sinusoidally modulated. From 2 to 8 weeks after the surgery amplitudes of pattern reversed ERGs were gradually reduced but as compared with those of the optic nerve sectioned cats the reduction was mild and more gradual, suggesting some rescuing effects of the nerve graft on axotomized ganglion cells from retrograde cell death.