Visual information transmission in cat retinal ganglion cells with regenerated axons through the peripheral nerve graft.

具有再生轴突的猫视网膜神经节细胞通过周围神经移植物传输视觉信息。

基本信息

批准号：
06455013
负责人：
FUKUDA Yutaka
金额：
$ 5.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Under anesthesia the optic nerve of adult cats was completely sectioned and an autologous sciatic nerve was grafted. Visual functions of the retinal ganglion cells with regenerated axons were studied first by recording single unit activities from regenerated axons and secondly by recording pattern reversed ERGs. Results are summarized below. (1) At about 60 days survival the animals were reanesthetized and immobilized. The grafted nerve was dissected into fine fascicles and single unit activities were recorded with silver wire electrodes. A total of 286 visually responsive units were recorded and they were classified into the following groups based on receptive field properties : 142 were Y cells, 113 were X cells, 19 were W cells and the rest 12 were unclassified. Receptive field center size was normal in most units except for central X cells with enlarged fields. There was a clear asymmetry in the occurrence of ON and Off center cells especially among X cells where there was no OFF center cells while many ON center cells were recorded. (2) After the same surgery of optic nerve section and nerve graft, cats were reanesthetized and ERGs were recorded in response to vertical gratings whose luminance was sinusoidally modulated. From 2 to 8 weeks after the surgery amplitudes of pattern reversed ERGs were gradually reduced but as compared with those of the optic nerve sectioned cats the reduction was mild and more gradual, suggesting some rescuing effects of the nerve graft on axotomized ganglion cells from retrograde cell death.

在麻醉下，完全切开了成年猫的视神经，并嫁接了自体坐骨神经。首先研究带有再生轴突的视网膜神经节细胞的视觉功能，通过记录来自再生轴突的单个单位活动，其次通过记录模式逆转ERG来研究。结果总结下面。（1）在大约60天生的生存时，将动物重新进行并固定。将移植的神经解剖为细筋膜，并用银线电极记录单个单位活动。总共记录了286个视觉响应单元，并根据接受场特性分类为以下组：142个是Y细胞，113个为X细胞，19个细胞为W细胞，其余12个未分类。除了具有扩大场的中央X细胞外，大多数单位的接受场中心大小是正常的。在没有中心细胞的X细胞中，在没有中心细胞的X细胞中，在中心细胞中发生了许多，而中心细胞的发生时，中心细胞的发生有明显的不对称性。（2）在相同的视神经截面和神经移植手术后，对猫进行了重新检查，并记录了ERG，以响应其亮度为正弦调节的垂直光栅。手术振幅从模式的振幅逐渐减少后，逐渐减少，但是与视神经截面猫的猫相比，还原性较小且逐渐逐渐，这表明神经移植物对逆行细胞死亡的轴突神经节细胞的某些营救作用。