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Visual information transmission in cat retinal ganglion cells with regenerated axons through the peripheral nerve graft.

具有再生轴突的猫视网膜神经节细胞通过周围神经移植物传输视觉信息。

基本信息

批准号：
06455013
负责人：
FUKUDA Yutaka
金额：
$ 5.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Under anesthesia the optic nerve of adult cats was completely sectioned and an autologous sciatic nerve was grafted. Visual functions of the retinal ganglion cells with regenerated axons were studied first by recording single unit activities from regenerated axons and secondly by recording pattern reversed ERGs. Results are summarized below. (1) At about 60 days survival the animals were reanesthetized and immobilized. The grafted nerve was dissected into fine fascicles and single unit activities were recorded with silver wire electrodes. A total of 286 visually responsive units were recorded and they were classified into the following groups based on receptive field properties : 142 were Y cells, 113 were X cells, 19 were W cells and the rest 12 were unclassified. Receptive field center size was normal in most units except for central X cells with enlarged fields. There was a clear asymmetry in the occurrence of ON and Off center cells especially among X cells where there was no OFF center cells while many ON center cells were recorded. (2) After the same surgery of optic nerve section and nerve graft, cats were reanesthetized and ERGs were recorded in response to vertical gratings whose luminance was sinusoidally modulated. From 2 to 8 weeks after the surgery amplitudes of pattern reversed ERGs were gradually reduced but as compared with those of the optic nerve sectioned cats the reduction was mild and more gradual, suggesting some rescuing effects of the nerve graft on axotomized ganglion cells from retrograde cell death.

麻醉下完全切断成年猫的视神经，移植自体坐骨神经。首先通过记录再生轴突的单个单位活动来研究再生轴突视网膜神经节细胞的视觉功能，然后通过记录图形反转ERG来研究再生轴突视网膜神经节细胞的视觉功能。研究结果总结如下。(1)存活约60d后，再麻醉并制动。将移植神经解剖成细束，用银丝电极记录单个单位的活动。共记录了286个视觉反应单位，并根据感受野特性将其分为以下组：142个为Y细胞，113个为X细胞，19个为W细胞，其余12个未分类。除中央X细胞视野扩大外，多数单位感受野中心大小正常。开中心细胞和偏离中心细胞的出现有明显的不对称性，特别是在X细胞中，没有偏离中心的细胞，但记录到许多中心上的细胞。(2)视神经切断术和神经移植物手术后，猫再次麻醉，利用正弦调制亮度的垂直栅格记录ERG。术后2~8周，模式反转ERG波幅逐渐降低，但与视神经切断组相比，幅度下降较轻，且较为缓慢，提示神经移植物对视神经切断后神经节细胞的逆行死亡有一定的挽救作用。