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Studies on Functional Evaluation of Regenerated Optic Nerve

再生视神经功能评价的研究

基本信息

批准号：
11694274
负责人：
FUKUDA Yutaka
金额：
$ 12.63万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11694274/
关键词：
optic nerve regeneration retina ganglion cell axon receptive field functional evaluation behavior

项目摘要

1. Facilitatory effects of intraocularly applied neurotrophins on survival and axonal regeneration of cat retina ganglion cells (Watanabe et al., 2002) : After intraocular applications of a mixture of BDNF, CNTF and forskolin, not only the survival but also the axonal regeneration by peripheral nerve graft was significantly enhanced in axotomized cat retinal ganglion cells. The effect was especially conspicous on beta cells.2. Capacities of visual information processing in axotomized cat retinal ganglion cells (Takao et al., 2002) : 5 and 14 days after optic nerve transection visual function of axotomized cat retina ganglion cells were evaluated. Most of the surviving cells revealed normal visual responses which enabled to classify into Y or X cells. However, the size of the receptive field center was shrunken in some of the axotomized cells.3. Quantitative evaluations of survival and axonal regeneration of retinal ganglion cells in bcl-2 overexpressed mice (Inouet et al., 2002) : In b … More cl-2 transgenic mice the survival of axotomzied ganglion cells was significantly higher than in wild type mice, whereas the axonal regeneration, assessed by peripheral nerve transplanation, was similar between the two. This result appears to be in conflict with the well known suggestion that bcl-2 has promoting effect on axonal regeneration of retinal ganglion cells as well as their survival.4. Survival promoting effects of electrical stimulation to the cut optic nerve on axotomized rertinal ganglion cells in rats (Morimoto et al., 2002) : Monophasic electrical plulses (50 μA, 50μs, At s , 20 Hz) applied for 2 hrs to the cut end of the optic nerve enhanced the one-week survival of retinal gangion cells up to 83 % from the control level (53 %).5. Behavioral evaluations of functional recovery of vision (Sasaki et al., 1999) : Hamsters having a reconstructed retino-collicular projection with peripheral nerve graft revealed visually guided behaviors when assessed by three different behavioral paradigms ; open field behavior, classical aversive conditioning and operant avoidance learning.6. Review works on degeneration, surival and axonal regeneration of cat retinal ganglion cells (Watanabe and Fukuda, 2002) : After summarizing our recent works on cat retinal ganglion cells, we reviewed the present status of the worldwide trials towards the protection of damaged retinal ganglion cells and the promotion of their axonal regeneration in adult mammals. Less

1.眼内施用的神经营养因子对猫视网膜神经节细胞的存活和轴突再生的促进作用（Watanabe等人，2002年）：在眼内应用BDNF、CNTF和forskolin的混合物后，不仅周围神经移植物的存活而且轴突再生在轴突切断的猫视网膜神经节细胞中显著增强。对β细胞的影响尤为显著.轴突切断的猫视网膜神经节细胞中视觉信息处理的能力（Takao等人，2002）：在视神经横切后5和14天，评价轴突切断的猫视网膜神经节细胞的视觉功能。大多数存活的细胞显示出正常的视觉反应，这使得能够分类为Y或X细胞。但部分切断轴突的细胞感受野中心缩小. bcl-2过表达小鼠中视网膜神经节细胞的存活和轴突再生的定量评价（Inouet et等人，2002年）：在B类 ...更多信息 CL-2转基因小鼠轴突切断的神经节细胞的存活率显著高于野生型小鼠，而通过外周神经移植评估的轴突再生在两者之间相似。这一结果似乎与众所周知的bcl-2对视网膜神经节细胞轴突再生及其存活具有促进作用的观点相矛盾.对大鼠中切断的视神经的电刺激对轴突切断的视网膜神经节细胞的存活促进作用（Morimoto等人，2002年）：在视神经切断端施加单相电脉冲（50 μA，50μs，At s，20 Hz）2小时，使视网膜神经节细胞的一周存活率从对照水平（53%）提高到83%。视觉功能恢复的行为评价（Sasaki等人，1999年版）：用周围神经移植重建视网膜丘投射的仓鼠在三种不同的行为范式评估时显示了视觉引导的行为;开放领域行为，经典厌恶条件反射和操作性回避学习。综述猫视网膜神经节细胞的变性、存活和轴突再生（Watanabe and Fukuda，2002）：在总结了我们最近对猫视网膜神经节细胞的研究之后，我们综述了世界范围内对成年哺乳动物受损视网膜神经节细胞的保护和促进其轴突再生的研究现状。少