STUDY ON THE RECONSTRUCTION OF VISUAL PATHWAYS

视觉通路重建的研究

基本信息

批准号：
03044097
负责人：
FUKUDA Yutaka
金额：
$ 6.4万
依托单位：
OSAKA UNIVERSITY MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03044097/
关键词：
Optic Nerve Axonal Regeneration Nerve Transplant Retino-collicular Projection Nerve Trophic Factor Retinal Ganglion Cells Visual Pathway Function Recovery 細胞外マトリクス網膜ー上丘路網膜会経節細胞

项目摘要

1. Studies on optic nerve regeneration in cats1) As a continuation of the previous study we recorded 260 visually responsive units from teased fibers of the sciatic nerve through which retinal ganglions cell axon regenerated. We confirmed the outnumber of Y cells than X and other cells and further found that among X cells there was no OFF center cell while in Y cells both ON and OFF were sampled. 2) After anterograde labeling with biocytin we studied the cross section of the regenerated optic axons and found that most of the axons were unmyelinated and the diameter range was smaller even among myelinated fibers as compared with the diameter range of normal optic axons in cats. 3) Morphological types of ganglions cells which survived for 2 months after optic nerve section were studied by a retrograde labeling method coupled with intracellular injections of Lucifer Yellow. alpha (Y)cells turned out to be the most resistant to axotomy over the other cells types.2. Functional recovery of v … More ision was studied in adult hamsters with a reconstructed retinocolliclar projection by the peripheral nerve transplant. In two hamsters out of four relative recovery of vision was obtained in avoidance score during the session of conditioned avoidance behavior using a light stimuli as a conditioning stimulus. For these two animals we succeed to trace regenerated retinal axons into the superior colliculus by an anterograde labeling with WGA-HRP. Furthermore we succeeded to obtain EEG desynchronization after photic stimulation into the eye which was bridged to the colliculus with a peripheral nerve graft.3. Effects of neurotrophic factors such BDNF, NT3, NT4 on axonal regeneration of retinal ganglion cells were studied 2-30 days after optic nerve section. Intracellular injections of neurobiotin revealed detailed morphology of regenerating axons such as recurring axons and side branches within the whole mount preparation. BDNF was found most effective to increase the number of recurring axons with occasional side branches. Less

1。关于CATS的视神经再生的研究1）作为先前研究的延续，我们记录了260个视觉响应单元，来自坐骨神经的嘲笑纤维，通过这些单位，仍将神经节细胞轴突重新生成。我们证实了Y细胞的外部数量比X和其他细胞的外部数量，并进一步发现，在X细胞中，在Y细胞中没有偏离中心细胞，而在Y细胞中进行了采样。 2）用生物细胞素进行行真标记后3）通过逆行标记方法和细胞内注射路西法黄色的逆行标记方法研究了神经节细胞的形态类型的神经节细胞。事实证明，α（Y）细胞是对其他细胞类型的轴切开术最抗性的2。 V的功能恢复V…在成年仓鼠中研究了更多的ISION，该仓鼠通过周围神经移植重建视网膜胶片投射。在有条件的回避行为中，使用光刺激作为调节性刺激，在四个相对恢复中的两次仓鼠中，避免得分获得了。对于这两只动物，我们通过WGA-HRP的顺行标记成功地将重生的视网膜轴突痕迹痕迹进入了上丘。此外，我们成功地获得了光学刺激进入眼睛后的脑电图，并用外周神经移植物桥接到丘神经。3。神经营养因子（例如BDNF，NT3，NT4）对视网膜神经节细胞轴突再生的影响在视神经段后2-30天进行了研究。对神经生物素的细胞内注射揭示了重生轴突的详细形态，例如重复出现的轴突和整个安装式制剂中的侧支。发现BDNF最有效地增加了具有偶尔侧支的重复轴突的数量。较少的