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Bcl-2 expression on germinal center lymphocytes modulated by the emperipolesis of follicular dendritic cells

滤泡树突状细胞伸入调节生发中心淋巴细胞上的 Bcl-2 表达

基本信息

批准号：
07670028
负责人：
TSUNODA Rikiya
金额：
$ 1.09万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-07670028/
关键词：
Follicular dendritic cells Germinal centers B cells Apoptosis Bcl-2 Fas Bーリンパ球樹状細胞

项目摘要

Germinal center (GC) B cells are highly susceptible to apoptosis. The cellular mechanism regulating this sensitivity, however has not yet been fully delineated. To investigate whether follicular dendritic cells (FDC) are capable of regulating the susceptibility to apoptosis of GC B cells, we constructed a GC model in vitro : emperipolesis of tonsillar B cells by FDC. We then analyzed the expression of apoptosis-related proteins (Bcl-2 and Fas) on the cells by three-color flow cytometry. B cells nonentrapped B cells were rescued for at least 18h and showed peculiar regulation of Gfas and Bcl-2. GC founder cells (CD38ィイD1+ィエD1, IgDィイD1+ィエD1 ; GCFC) and GC B cells (CD38ィイD1+ィエD1, IgDィイD1-ィエD1) showed approximately a twofold increased expression of Fas ; in contrast, mantle zone B cells (CD38ィイD1-ィエD1, IgDィイD1+ィエD1) and memory B cells (CD38ィイD1-ィエD1, IgDィイD1-ィエD1) showed no changes. Bcl-2 expression in mantle zone and memory B cells was reduced by approximately one-half ; however, GCFC and GC b cells continued to express little Bcl-2 and this did not change. Our findings strongly suggested that FDC play a part in the modulation of the susceptibility to apoptosis on B cells within GC.

生发中心（GC） B细胞对凋亡高度敏感。然而，调节这种敏感性的细胞机制尚未被充分描述。为了研究滤泡树突状细胞（follicular dendritic cells， FDC）是否能够调控GC B细胞的凋亡易感性，我们构建了体外GC模型：FDC诱导扁桃体B细胞凋亡。通过三色流式细胞术分析细胞凋亡相关蛋白（Bcl-2和Fas）的表达。未被包裹的B细胞至少抢救18小时，并表现出特殊的Gfas和Bcl-2调控。GC创始细胞（CD38 γ γ γ γ γ + γ γ γ γ + γ γ γ + γ γ γ + γ γ）和GC B细胞（CD38 γ γ + γ γ + γ γ + γ γ - γ γ） Fas表达增加约2倍；而地幔区B细胞（CD38 γ γ γ γ γ - γ γ γ γ + γ γ γ γ + γ γ γ）和记忆区B细胞（CD38 γ γ - γ γ, γ γ γ γ - γ γ）则无明显变化。Bcl-2在套区和记忆B细胞中的表达减少了约一半；然而，GCFC和GC b细胞继续表达少量Bcl-2，并且这种情况没有改变。我们的研究结果强烈提示FDC在GC内B细胞的凋亡敏感性调节中起一定作用。