Follicular dendritie cells and RAG-1&RAG-2 expression on germinal center B cells.

滤泡树突细胞和 RAG-1

• 批准号: 10670023
• 负责人: TSUNODA Rikiya
• 金额: $ 1.66万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670023/
• 关键词: RAG-1&RAG-2; Germinal centers; Mature B cells; Follicular dendritic cells; Bリンパ球; RAG_1 & RAG_2

In our previous project, we have reported that emperipolesis of germinal center B cells (GCB) is a novel functional marker of follicular dendritic cells (FDC) and FDC can modulate the susceptibility of apoptosis of GCB through this reaction. In this study, we aimed to clear using RT-PCR method whether FDC also regulate the re-expression of RAG-1 ＆ RAG-2 on GCB.We continue our research still for the final report, since the hypothesis seems to be accepted that the re-expression of RAG-1 ＆ RAG-2 on GCB is doubtful in vivo system. Our preliminary results are ; (1) The clear expression of RAG-1&RAG-2 could not be detected on tonsillar GCB.(2) The re-expression of RAG-1&RAG-2 could be often induced on follicular B cells. (3) FDC could not modulate this re-expression. (4) The survey of RAG-1&RAG-2 expression on the pathological cell lines, all follicular lymphoma (3/3) and some diffuse large B cell lymphoma (centroblastic 1/9, T cell/histiocyte-rich 5/7) showed positivity (Nakamura et al.)In summary, FDC might not be involved in the re-expression of RAG-1＆ RAG-2 on GCB.The examination of B-cell lines, however, suggests that RAG-1&RAG-2 expression have some relationship of malignant process within germinal centers. Therefor this research should be continued for clarify the role of RAG-1&RAG-2 on B cell malignant process.

在我们的前期研究中，我们报道了生发中心B细胞（GCB）的凋亡是滤泡树突状细胞（FDC）的一种新的功能性标志，FDC可以通过这种反应调节GCB的凋亡易感性。本研究的目的是通过RT-PCR的方法来明确FDC是否也能调节GCB上RAG-1和RAG-2的再表达。由于RAG-1和RAG-2在GCB上的再表达在体内系统中是可疑的这一假设似乎已经被接受，因此我们继续我们的研究以获得最终的报告。我们的初步研究结果是：（1）RAG-1和RAG-2在扁桃体GCB上没有明确的表达。(2)RAG-1和RAG-2在卵泡B细胞上常被诱导重新表达。(3)FDC不能调节这种再表达。(4)RAG-1和RAG-2在病理细胞系上的检测，所有滤泡性淋巴瘤（3/3）和一些弥漫性大B细胞淋巴瘤（中心母细胞型1/9，富含T细胞/组织细胞型5/7）显示阳性（中村等）。总之，FDC可能不参与RAG-1和RAG-2在GCB上的再表达，但对B细胞系的检测表明RAG-1和RAG-2的表达与生殖中心的恶性过程有一定的关系。因此，RAG-1和RAG-2在B细胞恶性过程中的作用有待进一步研究。

项目成果

Y.Hashimoto, N.Nakamura, et al.: "A study of the expression of recombination activating gene (RAG) on follicular lymphoma and diffuse large B cell lymphoma (japanese)."J.Japanese RES. 40(2). 71 (2000)

Y.Hashimoto、N.Nakamura 等人：“滤泡性淋巴瘤和弥漫性大 B 细胞淋巴瘤中重组激活基因 (RAG) 表达的研究（日本）。”J.Japan RES。