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Production of brassinolide-like substances by fungi.

通过真菌生产芸苔素内酯类物质。

基本信息

批准号：
59480057
负责人：
OISHI Kunio
金额：
$ 3.84万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480057/
关键词：
Brassinolide Oleic acid Monoolein Fungi Plant hormone イネラミナジョイントテスト

项目摘要

Brassinolide activity was screened in methanol extracts of the cells of 1098 fungal strains, accordind to rice lamina inclination test. Thirty two strains were found to show as high as 10 ng/g wet cell-level activity. They belong to Cunninghamella, Penicillium, Trichoderma, Aspergillus, Cladosporium and others. Productivity of brassinolide activity was present prejudically among fungal genera, but commonly in the genus. The activity per cells did not depend on the composition of culture media even when various steroidal compounds were added. The activity in methanol extracts was separated mainly into two fractions in silica gel column chromatography; one which was more hydrophobic than brassinolide and the other which was markedly hydrophilic. The former one was the major substance, weighing 60% of the total active fractions. Four and a half g of the hydrophobic substance was purified from 7 kg wet cells of Cunninghamella blakesleeana by successive extraction with methanol, ethyl acetate, and acetone, column chromatographys on almina and silica gel, and preparative HPLC. The substance was identified by TLC and GC/MS as monoolein. Commercial products of monoolein from plants also showed brassinolide activity. Activity of monoolein was one twenty thousandth of brassinolide and was detected at a level of 10 <micro> g/ml. Oleic acid was tenth as active as monoolein. Physiological role of oleic acid and its derivatives in plant was discussed.

采用水稻叶片倾斜试验，对1098株真菌细胞甲醇提取物进行抑菌活性筛选。发现32个菌株显示高达10纳克/克湿细胞水平的活性。属于小克银汉霉属、青霉属、木霉属、曲霉属、枝孢属等。芸苔素酶活性的生产力在真菌属间存在一定的差异，但在属内普遍存在。每细胞的活性不依赖于培养基的组成，即使当各种甾体化合物被添加。甲醇提取物中的活性在硅胶柱色谱中主要分离成两个级分;一个比油菜素苷更疏水，另一个明显亲水。前者为主要活性成分，占总活性部位的60%。从7 kg的小克银汉霉湿细胞中通过用甲醇、乙酸乙酯和丙酮连续提取，在氧化铝和硅胶上进行柱层析，以及制备型HPLC纯化4.5 g疏水性物质。通过TLC和GC/MS鉴定该物质为单油酸甘油酯。来自植物的单油酸甘油酯的商业产品也显示油菜素酶活性。单油酸甘油酯的活性是油菜素的二万分之一，并且在10 μ g/ml的水平下检测<micro>。油酸的活性是单油酸甘油酯的十分之一。论述了油酸及其衍生物在植物中的生理作用。