BIOLOGICAL AND MOLECULAR BIOLOGICAL STUDIES OF ANIMAL PARVOVIRUSES

动物细小病毒的生物学和分子生物学研究

• 批准号: 59480083
• 负责人: GOTO Hitoshi
• 金额: $ 4.29万
• 依托单位: OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480083/
• 关键词: Parvovirus; Feline; Mink; Canine; Biological Properties; ウイルスDNA

A similarity between feline panleukopenia (FPL), mink enteritis (ME) and canine parvoviruses (CP) in virological and serological properties was described by many investigators. While some differences among the viruses were demonstrated on the biological property. Especially, CPV infection has a wide distribution throughout the world since the year 1978, and this infection has received special attention as a new canine virus disease. This paper concerns the comparative studies of biological and molecular biological properties among the viruses.Mev had about the samw properties as FPLV in cross-HI tests, in the propagating ability of viruses in MDCK calls and in the hemagglutinability, whereas the heat stability at 80゜C, and the restriction enzyme digestion patterns of the viral replicating form (RF) DNAs were similar to those of CPV. The nucleotide sequence of cloned MEV RF-DNA was analysed and the gene organization was determined. The sequence determined was 3,510 nucleotides long, which shared 70% of whole MEV DNA.In the DNA homology, and in the amino acid sequence, among MEV, FPLV and CPV, MEV showed slightly higher homology with FPLV than CPV. The cloned DNA was introduced into a feline kidney cell line (CRFK), by transfection, and expression of the DNA in the cells was examined.Intranuclear inclusion bodies, which resembled to those found in the MEV-infected cell nuclei, were observed in the transfected cells 48 hours post transfection (p.t.). Viral capsid proteins VP-2' and VP-2 were also detected in the cells. However, it was failed to recover infectious MEV virions from the transfected cell cultures havested for 4 to 7 days p.t. These results indicate that the cloned MEV RF-DNA has been able to express at least some of its functions in CRFK cells.

猫泛白细胞减少症（FPL）、水貂肠炎（ME）和犬细小病毒（CP）在病毒学和血清学特性上有相似性。但在生物学特性上存在一定的差异。特别是自1978年以来，CPV感染在世界范围内广泛分布，作为一种新的犬病毒性疾病受到特别关注。本文对这两种病毒的生物学和分子生物学特性进行了比较研究，结果表明，Mev在交叉HI试验、MDCK细胞增殖能力和血凝性方面与FPLV相似，而在80 ℃热稳定性和病毒复制型（RF）DNA的酶切图谱方面与CPV相似。对克隆的MEV RF-DNA进行了核苷酸序列分析，确定了基因组结构。结果表明，MEV与FPLV、CPV的同源性分别为70%和3,510个核苷酸，MEV与FPLV的同源性略高于CPV。通过转染将克隆的DNA导入猫肾细胞系（CRFK）中，检测细胞中DNA的表达，转染后48小时（p.t.），在转染细胞中观察到与MEV感染细胞核中发现的包涵体相似的核内包涵体。在细胞中还检测到病毒衣壳蛋白VP-2'和VP-2。然而，未能从p.t. 4至7天的转染细胞培养物中回收感染性MEV病毒粒子。这些结果表明，克隆的MEV RF-DNA已经能够在CRFK细胞中表达其至少一些功能。

项目成果

Goto,H.;Hirano,T.;Watanabe,K.;Shinagawa,M.;Ichijo,S.;Shimizu,K.: Japanese Journal of Veterinary Science. 46. 519-526 (1984)

后藤，H.；平野，T.；渡边，K.；品川，M.；一条，S.；清水，K.：日本兽医学杂志。

Goto,H.;Uchida,E.;Ichijo.S.;Shimizu,K.;Morohoshi,Y.;Nakano,K.: "Experimental infection of canine parvovirus in specific pathogen-free cats." Japanese Journal of Veterinary Science. 46. 729-731 (1984)

Goto，H.；Uchida，E.；Ichijo.S.；Shimizu，K.；Morohoshi，Y.；Nakano，K.：“在无特定病原体的猫中进行犬细小病毒的实验感染。”

Goto,H.;Taneda,A.;Shinagawa,M.;Hama,E.: Japanese Journal of Veterinary Science. 48. 1025-1028 (1986)

Goto，H.；Taneda，A.；品川，M.；Hama，E.：日本兽医学杂志。

Goto,H.;Hirano,T.;Watanabe,K.;Shinagawa,M.;Ichijo,S.;Shimizu,K.: "Comparative studies of physicochemical and biological properties between canine parvovirus and feline panleukopenia virus." Japanese Journal of Veterinary Science. 46. 519-526 (1984)

Goto，H.；Hirano，T.；Watanabe，K.；品川，M.；Ichijo，S.；Shimizu，K.：“犬细小病毒和猫泛白细胞减少症病毒之间的理化和生物学特性的比较研究。”

Goto,H.;Taneda,A.;Shinagawa,M.;Hama,E.: "Biological and physical comparison of mink enteritis virus with feline panleukopenia virus and canine parvovirus." Japanese Journal of Veterinary Science. 48. 1025-1028 (1986)

Goto，H.；Taneda，A.；品川，M.；Hama，E.：“水貂肠炎病毒与猫泛白细胞减少症病毒和犬细小病毒的生物学和物理比较。”