BIOLOGICAL AND MOLECULAR BIOLOGICAL STUDIES OF ANIMAL PARVOVIRUSES

由许多研究者描述的微生物学和血清学特性之间的相似性（FPL）、微型肠道菌（ME）和阴道寄生虫（CP）。当病毒在生物学特性上被证明有一定的差异时。特别是， CPV感染自1978年以来一直在推动世界的广泛传播，而这种感染也收到了一种新的癌症病毒疾病的特殊关注。本文讨论了病毒中的生物学和分子生物学特性的比较研究。Mev有关于FPLV在交叉HI测试中的Samw特性，在MDCK呼叫中的病毒传播能力和在热凝性中，在80 ° C的热稳定性中，以及在病毒复制形式的限制酶数字模式（RF）DNA与这些CPV相似。克隆MEV RF的核苷酸序列-DNA被分析，基因组织被确定。该序列确定为3，510个长核，共享70%的MEV DNA，在DNA同源学中，在氨基酸序列中，MEV、FPLV和CPV中， MEV显示了比FPLV更高的同源学。克隆的DNA被引入到一个细胞肾脏细胞线（CRFK）中，通过转染和对细胞中DNA的表达进行了测试。线粒体内包含体，在MEV感染的细胞核中发现了那些，在转染细胞中观察到了48小时后转染（p.t.）。病毒性囊体蛋白VP-2 '和VP-2也在细胞中检测到。然而，在4至7天内，它未能恢复从被转染的细胞文化中感染的MEV小提琴。这些结果表明，克隆的MEV RF-DNA至少可以在CRFK细胞中表现出一些功能。

A similarity between feline panleukopenia (FPL), mink enteritis (ME) and canine parvoviruses (CP) in virological and serological properties was described by many investigators. While some differences among the viruses were demonstrated on the biological property. Especially, CPV infection has a wide distribution throughout the world since the year 1978, and this infection has received special attention as a new canine virus disease. This paper concerns the comparative studies of biological and molecular biological properties among the viruses.Mev had about the samw properties as FPLV in cross-HI tests, in the propagating ability of viruses in MDCK calls and in the hemagglutinability, whereas the heat stability at 80゜C, and the restriction enzyme digestion patterns of the viral replicating form (RF) DNAs were similar to those of CPV. The nucleotide sequence of cloned MEV RF-DNA was analysed and the gene organization was determined. The sequence determined was 3,510 nucleotides long, which shared 70% of whole MEV DNA.In the DNA homology, and in the amino acid sequence, among MEV, FPLV and CPV, MEV showed slightly higher homology with FPLV than CPV. The cloned DNA was introduced into a feline kidney cell line (CRFK), by transfection, and expression of the DNA in the cells was examined.Intranuclear inclusion bodies, which resembled to those found in the MEV-infected cell nuclei, were observed in the transfected cells 48 hours post transfection (p.t.). Viral capsid proteins VP-2' and VP-2 were also detected in the cells. However, it was failed to recover infectious MEV virions from the transfected cell cultures havested for 4 to 7 days p.t. These results indicate that the cloned MEV RF-DNA has been able to express at least some of its functions in CRFK cells.