Studies on enterohepatic axis

肠肝轴研究

• 批准号: 59480254
• 负责人: SHIMA Kenji
• 金额: $ 4.16万
• 依托单位: Tokushima University (1985-1986)Osaka University (1984)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480254/
• 关键词: Jejunectomized rats; Liver perfusion; Gut hormones; cAMP; phosphopylase; Primary cultured hepatocyte; Enteroinsular axis

Effects of the intestinal tract on carbohydrate metabolism was stueied in respect of its direct action on the metabolism in the liver and of the indirect effect through the pancreatic endocrine functions.1. direct effects(1) In the jejunectomized rats which had been nurished for a week by total parenteral nutrition the response of plasma glucose and IRI to an intraperitoneal glucose load was significantly higher than in the controls.(2) Effects of various gut hormones on cAMP production, phosphorylase activity and glucose output were investigated in primary cultured hepatocytes. Glucagon, secretin and VIP augmented these three parameters, but others such as GRF, GIP and PHI did not stimulate all three.2. A new candidate for incretin-like substance and a physiological significance of the enteroinsular axis. In response to an oral glucose load the plasma GLP-1 immunoreactivity level was increased in man and GLP-1 stimulated IRI release from the perfused rat pancreas. This might be the new incretin. It became clear that the enteroinsular axis acted on the pancreatic A cells as well on the basis of the fact that an increase in plasma glucagon level in response to arginin was augmented after an oral glucose load.

从肠道对肝脏代谢的直接作用和通过胰腺内分泌功能的间接作用两方面研究了肠道对碳水化合物代谢的影响。直接效应(1)全胃肠外营养喂养一周后，空肠切除大鼠的血糖和IRI对腹水葡萄糖负荷的反应明显高于对照组。(2)观察了不同胃肠激素对原代培养肝细胞cAMP生成、磷酸酶活性和葡萄糖产量的影响。结论：1.胰高血糖素、促胰液素和血管活性肠肽对上述三项指标均有增强作用，而GRF、GIP和PHI对上述三项指标均无刺激作用。胰岛素样物质的新候选者和肠岛轴的生理意义。口服葡萄糖负荷后，人血浆GLP-1免疫反应水平升高，GLP-1可刺激大鼠胰腺IRI的释放。这可能是新的胰岛素。很明显，肠岛轴也作用于胰腺A细胞，这是基于口服葡萄糖负荷后精氨酸引起的血浆胰高血糖素水平增加的事实。

项目成果

Ichiro Shimizu: "Effect of glucagon-like peptide-1 and -2 glycogenolysis in cultured rat hepatocytes" Biomedical Research. 7. 431-436 (1986)

Ichiro Shimizu：“培养大鼠肝细胞中胰高血糖素样肽-1 和-2 糖原分解的影响”生物医学研究。

Chizuko Ohboshi: "CO-existence of glucagon-like peptide-1 in a commercial glucagon preparation" Journal of the Japan Diabetes Society. 30. 29-34 (1987)

Chizuko Ohboshi：“商业胰高血糖素制剂中胰高血糖素样肽-1 的共存”，日本糖尿病学会杂志。

Kenji Shima: Diabetes Research and Clinical Practice. 1. 265-271 (1986)

Kenji Shima：糖尿病研究和临床实践。

Yasuo Akanuma: BIOMEDICAL RESEARCH FOUNDATION, TOKYO. PEPTIDE HORMONES IN PANCREAS 6, 331 (1986)

Yasuo Akanuma：生物医学研究基金会，东京。

太田康幸 編: "消化器病学の進歩'85" 日本医学館, 333 (1985)

太田康之主编：“Advances in Gastroenterology 85” 日本医学博物馆，333（1985）