High-throughput genotyping system

特定候选基因的核苷酸变异特征(单核苷酸多态-基因分型)是生态遗传学和育种中研究适应性遗传变异的标准方法。具体地说，分析关键调控基因沿环境梯度的遗传变异是了解种群如何在面对气候变化的情况下适应不断变化的环境条件的先决条件。除了DNA多态，与基因表达变化相关的表观遗传变异似乎促进了长期的进化适应，使其能够对环境变化做出快速和可塑性的反应。对于最先进的出版物，使用不断增加的基因标记进行大规模分析是必要的。准确测量聚合酶链式反应衍生的扩增片段的质谱仪是对遗传和表观遗传标记进行目标分析的标准程序。它将使我们能够以具有成本效益的方法分析遗传以及表观遗传变异及其在自然和繁殖种群的当地适应中的作用，并向其他大学机构提供这项服务。例如，我们将能够开发、测试和设计各种动植物物种的分析方法，并在景观水平上对中性和适应性遗传多样性以及表观遗传多样性进行大规模监测。不同院系(林业、生物学、农业科学)和哥廷根大学综合育种研究中心使用该系统将进一步促进大学内部的跨学科合作。

The characterization of nucleotide variation in specific candidate genes (Single Nucleotide Polymorphism-genotyping) is a standard method for the study of adaptive genetic variation in ecological genetics and breeding. Specifically, the analysis of genetic variation in key regulatory genes along environmental gradients is the prerequisite to understand how populations can adapt to changing and variable environmental conditions in the face of climate change. In addition to DNA polymorphisms, epigenetic variation related to changes in gene expression seems to facilitate long-term evolutionary adaptations enabling a rapid and plastic response to environmental changes. For state of the art publications, large-scale analyses with a continuously increasing number of gene markers are necessary. Mass spectrometry to accurately measure PCR-derived amplicons is a standard procedure for the targeted analysis of genetic and epigenetic markers. It would enable us to analyse genetic well as epigenetic variation and their role in local adaptation in natural and breeding populations with a cost effective approach and to offer this service to other university institutions. For example, we will be able to develop, test and design assays for a variety of plant and animal species and to perform large-scale monitoring of neutral and adaptive genetic diversity as well as epigenetic diversity at a landscape level.The use of the system by Departments of different Faculties (Forestry, Biology, Agricultural Sciences) and within the Center of Integrated Breeding Research at the University of Göttingen would further stimulate interdisciplinary collaborations within the University.