High-throughput genotyping system

高通量基因分型系统

• 批准号: 437098084
• 金额: --
• 依托单位: Georg-August-Universität Göttingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/437098084?language=en
• 关键词: throughput; genotyping; system

The characterization of nucleotide variation in specific candidate genes (Single Nucleotide Polymorphism-genotyping) is a standard method for the study of adaptive genetic variation in ecological genetics and breeding. Specifically, the analysis of genetic variation in key regulatory genes along environmental gradients is the prerequisite to understand how populations can adapt to changing and variable environmental conditions in the face of climate change. In addition to DNA polymorphisms, epigenetic variation related to changes in gene expression seems to facilitate long-term evolutionary adaptations enabling a rapid and plastic response to environmental changes. For state of the art publications, large-scale analyses with a continuously increasing number of gene markers are necessary. Mass spectrometry to accurately measure PCR-derived amplicons is a standard procedure for the targeted analysis of genetic and epigenetic markers. It would enable us to analyse genetic well as epigenetic variation and their role in local adaptation in natural and breeding populations with a cost effective approach and to offer this service to other university institutions. For example, we will be able to develop, test and design assays for a variety of plant and animal species and to perform large-scale monitoring of neutral and adaptive genetic diversity as well as epigenetic diversity at a landscape level.The use of the system by Departments of different Faculties (Forestry, Biology, Agricultural Sciences) and within the Center of Integrated Breeding Research at the University of Göttingen would further stimulate interdisciplinary collaborations within the University.

单核苷酸多态性基因分型（SingleNucleotidPolymorphism-genotyping）是生态遗传学和育种学中研究适应性遗传变异的标准方法。具体而言，分析关键调控基因沿着环境梯度的遗传变异，是了解面对气候变化，种群如何适应不断变化和多变的环境条件的前提。除了DNA多态性，与基因表达变化相关的表观遗传变异似乎有助于长期的进化适应，从而对环境变化做出快速和可塑性的反应。对于最新技术水平的出版物，需要使用数量不断增加的基因标记进行大规模分析。准确测量PCR衍生扩增子的质谱法是遗传和表观遗传标记靶向分析的标准程序。它将使我们能够分析遗传和表观遗传变异及其在自然和繁殖种群中的局部适应作用，并以具有成本效益的方法为其他大学机构提供这项服务。例如，我们将能够开发、测试和设计针对各种植物和动物物种的检测方法，并在景观层面对中性和适应性遗传多样性以及表观遗传多样性进行大规模监测。不同部门对该系统的使用学院（林业，生物学，农业科学）和哥廷根大学综合育种研究中心内的合作将进一步促进大学内的跨学科合作。