Study on the polymer synthesis for specific separation of oligomer using inclusion phenomena

利用包合现象特异性分离低聚物的聚合物合成研究

• 批准号: 60470111
• 负责人: TAKEMOTO Kiichi
• 金额: $ 4.86万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60470111/
• 关键词: High performance liquid chromatography; HPLC; nucleic acid; nucleic acid bases; nucleoside; nucleotide; oligonucleotide; complementaty bases; オリゴヌクレオチド; 核酸; オリゴマー; 分離; 相互作用

The present project concerns the preparation of the high performance liquid chromatography (HPLC) resins containing nucleic ac d derivatives which can be applied to HPLC system for nucleic ac d fragments using specific interaction between nucleic acid bases.In biochemical, medical, and biotechnological fields, separation and analysis of nucleic acids and their fragments are very important problems. In the field of biochemistry, the HPLC procedure has been developed for analysis of the nucleic acid fragments. It is known that nucleic acids form the polymer complex by the specific interaction caused by stacking and hydrogen bonding interactions. Therefore, the application of the specific interaction between nucleic acid bases for the HPLC system may give a very useful HPLC system for the analysis of the uncleic acid components.Nucleic acid base and nucleoside derivatives were bonded successfully to 3-aminopropylsilanized silica and silica gel. These resins were found to be useful as the columns of HPLC for the selective separation of oligoethyleneimine derivatives having pendant thymine or adenine derivatives. These resines were also found to give excellent complementary separation of nucleic acid bases, nucleosides, nucleotides, and oligonucleotides.

本课题主要研究了含核酸衍生物的高效液相色谱树脂的制备，该树脂可应用于利用核酸碱基之间的特异性相互作用来检测核酸片段的高效液相色谱体系。在生物化学、医学和生物技术领域，核酸及其片段的分离和分析是一个非常重要的问题。在生物化学领域，高效液相色谱法已被用于核酸片段的分析。已知核酸是通过堆积和氢键相互作用引起的特定相互作用形成聚合物复合物的。因此，将核酸碱基间的特异性相互作用应用于高效液相色谱体系中，可以为分析单链酸成分提供一个非常有用的高效液相色谱体系。核酸碱基和核苷衍生物成功地与3-氨基丙基硅烷化二氧化硅和硅胶结合。发现这些树脂可作为高效液相色谱柱用于选择性分离具有垂载胸腺嘧啶或腺嘌呤衍生物的低聚乙烯亚胺衍生物。这些树脂还被发现对核酸碱基、核苷、核苷酸和寡核苷酸具有很好的互补分离作用。

项目成果

S,Nagae, et al.: "Application of nucleic acid base containing polymers to high performance liquid chromatography" Journal of Polymer Science, Polymer Chemistry Edition,. (1988)

S，Nagae，等人：“含核酸碱基的聚合物在高效液相色谱中的应用”，高分子科学杂志，高分子化学版，。

Y. Inaki, et. al.: Nucleic Acids. s19. 45-48 (1988)

Y. Inaki 等。

Y. Inaki, et. al.: Applied Bioactive Polymeric System. (1988)

Y. Inaki 等。

Yoshiaki INAKI: Journal of Polymer Science:Polymer Chemistry Edition.

稻木义明：高分子科学杂志：高分子化学版。

S. Nagae, et al.: Journal of Polymer Science, Polymer Chemistry Edition. (1988)

S. Nagae 等人：《高分子科学杂志》，高分子化学版。