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Nutritional physiological studies on the pancreas digestive enzyme secretion system in chicks.

雏鸡胰腺消化酶分泌系统的营养生理研究。

基本信息

批准号：
60480084
负责人：
OKUMURA Jun-ichi
金额：
$ 4.1万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

A new collection method for pancreatic juice in chickens was developed. The wing vein injection of cholecystokinin had an immediate effect on pancreatic enzyme secretion and this response was in a dose dependent fashion, which seemed to be parallel to a dose of 0.06 and 0.15 IU, while 0.30 IU CCK seemed to have a selective stimulation favoring a greatest increase in chymotrypsinogen followed by amylase and trypsinogen. A steady, relatively small basal output of amylase, trypsinogen and chymotrypsinogen occured when the end products of digestion (glucose and various amino acids except phenylalanine) was administered individually. Phenylalanine injection stimulated significantly the secretion of amylase and trypsinogen. When the individual amino acid was injected with cholecystokinin, the pancreatic enzyme secretion was stimulated synergetically and the response of each enzyme was different depending on the kind of amino acid injected. These results suggested the wing vein injection of CCK alone or in combination with end products of digestion had the specific induction of a particular enzyme secretion. Chicks fed a diet with 10 or 100 ppm soybean trypsin inhibitor (SBTI) showed a similar body weight gain, but had heavier pancreas than chicks fed a diet without SBTI. The heavier pancreas showed hypertrophy in acinar cells which was due to enlargement and increased number of rough endoplasmic reticulum. The zymogen granule of chicks fed SBTI was small in number but showed enlargement tendency. It was suggested that the change of acinar cell by SBTI was introduced by oversecretion of zymogen granules. The pancreatic digestion enzyme secretion response pattern of chicks fed SBTI was similar to that of chicks fed no SBTI. Isozymes of pancreas digestive enzymes of chicks fed SBTI was studied.

开发了一种新的鸡胰液收集方法。翼静脉注射胆囊收缩素对胰酶分泌有立竿见影的作用，并且这种反应呈剂量依赖性方式，这似乎与0.06和0.15 IU的剂量平行，而0.30 IU CCK似乎具有选择性刺激，有利于胰凝乳蛋白酶原的最大增加，其次是淀粉酶和胰蛋白酶原。当消化终产物（葡萄糖和除苯丙氨酸外的各种氨基酸）单独施用时，淀粉酶、胰蛋白酶原和糜蛋白酶原的基础输出量稳定且相对较小。苯丙氨酸注射液显着刺激淀粉酶和胰蛋白酶原的分泌。当单独的氨基酸与缩胆囊素一起注射时，胰酶的分泌被协同刺激，并且每种酶的反应根据注射的氨基酸的种类而不同。这些结果表明，单独或与消化终产物组合的CCK的翼静脉注射具有特定酶分泌的特异性诱导。饲喂含有 10 或 100 ppm 大豆胰蛋白酶抑制剂 (SBTI) 的日粮的小鸡表现出相似的体重增加，但其胰腺比饲喂不含 SBTI 日粮的小鸡更重。较重的胰腺显示腺泡细胞肥大，这是由于粗面内质网增大和数量增加所致。饲喂SBTI的雏鸡酶原颗粒数量较少，但有增大趋势。推测SBTI对腺泡细胞的改变是由酶原颗粒的过度分泌引起的。饲喂 SBTI 的雏鸡的胰腺消化酶分泌反应模式与未饲喂 SBTI 的雏鸡相似。研究了饲喂 SBTI 的雏鸡胰腺消化酶的同工酶。