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STUDIES ON THE IMPROVEMENT OF PRODUCTION EFFICIENCY OF POULTRY USING ANTISENSE NUCLEOTIDES

利用反义核苷酸提高家禽生产效率的研究

基本信息

批准号：
10556062
负责人：
OKUMURA Jun-ichi
金额：
$ 8.58万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN.In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.The interactive effect between varying fetal calf serum concentrations (1% and 10% in the medium) and transfection treatment with antisense and sense myostatin oligodeoxynucleotide (ODN) on protein synthesis of chicken embryo myoblasts was examined. The significant interaction between varying fetal calf serum concentrations and transfection treatment was observed. The cationic liposome did not affect myoblast protein synthesis. Protein synthesis of chicken embryo myoblasts was significantly decreased by antisense myostatin ODN regardless of varying fetal calf serum concentrations in the medium. These results suggest that a decrease in myostatin reduced protein synthesis of chicken embryo myoblasts and that the influence of myostatin on protein synthesis would be independent of either IGF-I or IGF-II.

我们研究了 IGFBP-2 反义寡脱氧核苷酸 (ODN) 对雏鸡组织 IGFBP-2 基因表达的影响。将反义IGFBP-2 ODN直接注射到肝脏或脑室中。对照鸟注射媒介物。注射反义ODN后2小时，肝脏IGFBP-2基因表达量下降至对照的约30%。注射反义ODN的鸡脑中，注射2小时后IGFBP-2 mRNA水平没有变化，注射6小时后下降至对照的约60%。这些结果表明，通过施用反义ODN成功抑制了肝脏和脑中IGFBP-2基因的表达，并且与脑相比，反义ODN快速抑制了肝脏IGFBP-2基因表达。不同胎牛血清浓度（培养基中1%和10%）与反义和正义肌生长抑制素寡脱氧核苷酸（ODN）转染处理之间的相互作用效应研究了鸡胚成肌细胞蛋白质合成的影响。观察到不同胎牛血清浓度和转染治疗之间的显着相互作用。阳离子脂质体不影响成肌细胞蛋白质合成。无论培养基中胎牛血清浓度如何变化，反义肌生长抑制素ODN均显着降低鸡胚成肌细胞的蛋白质合成。这些结果表明，肌生长抑制素的减少减少了鸡胚成肌细胞的蛋白质合成，并且肌生长抑制素对蛋白质合成的影响将独立于IGF-I或IGF-II。