STUDIES ON THE IMPROVEMENT OF PRODUCTION EFFICIENCY OF POULTRY USING ANTISENSE NUCLEOTIDES

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We have examined the influence of antisense oligo deoxynucleotide (ODN) of IGFBP-2 on tissue IGFBP-2 gene expression in chicks. Antisense IGFBP-2 ODN was directly injected into the liver or cerebroventricle. Control birds were injected with vehicle. The hepatic IGFBP-2 gene expression was decreased to approximately 30% of the control at 2 h after injection of antisense ODN.In the brain of chickens injected with antisense ODN, IGFBP-2 mRNA level did not change after 2 h of injection and decreased to approximately 60% of the control after 6 h of injection. These results showed that the expression of IGFBP-2 gene in the liver and brain was successfully suppressed by administrating antisense ODN and that hepatic IGFBP-2 gene expression was quickly suppressed by antisense ODN compared with the brain.The interactive effect between varying fetal calf serum concentrations (1% and 10% in the medium) and transfection treatment with antisense and sense myostatin oligodeoxynucleotide (ODN) on protein synthesis of chicken embryo myoblasts was examined. The significant interaction between varying fetal calf serum concentrations and transfection treatment was observed. The cationic liposome did not affect myoblast protein synthesis. Protein synthesis of chicken embryo myoblasts was significantly decreased by antisense myostatin ODN regardless of varying fetal calf serum concentrations in the medium. These results suggest that a decrease in myostatin reduced protein synthesis of chicken embryo myoblasts and that the influence of myostatin on protein synthesis would be independent of either IGF-I or IGF-II.