Structure and Function of Antigen Genes in Animal Mycoplasmas

动物支原体抗原基因的结构和功能

• 批准号: 60480088
• 负责人: HARASAWA Ryo
• 金额: $ 0.38万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480088/
• 关键词: Mycoplasma; 抗原遺伝子

The aim of this research is (1) to clone in Escherichia coli the mycoplasma genes encoding antigenic determinants, and (2) to try to express the mycoplasmal genes in Escherichia coli or in mammalian cultured cells for the purpose of development of vaccines against mycoplasma infections.The first year, we prepared mycoplasma DNA and vector DNA, and identified the antigenic determinants of mycoplasmas by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. We obtained a mycoplasma gene library by using plasmid vector, and screened the clones producing antigenic products but only to fail. The second year, we tried to prepare a gene library of Mycoplasma pulmonis by using lambda gt 11 phage vector and finaly obtained ten clones positive in antigen production. These clones might be useful for development of component vaccines against Mycoplasma pulmonis infections. The fine structure of genes encoding antigenic determinant is currently being investigated.

本研究的目的是（1）在大肠杆菌中克隆编码支原体抗原决定簇的基因，（2）尝试在大肠杆菌或哺乳动物培养细胞中表达支原体基因，以开发抗支原体感染的疫苗。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹法鉴定支原体抗原决定簇。利用质粒载体构建了支原体基因文库，并筛选了产生抗原产物的克隆，但均失败。第二年，我们尝试用λ gt 11噬菌体载体制备肺支原体基因文库，最终获得10个抗原产生阳性克隆。这些克隆可能有助于开发抗肺支原体感染的组分疫苗。目前正在研究编码抗原决定簇的基因的精细结构。

项目成果

川瀬雅子 他: 組識培養. 12. 298-303 (1986)

Masako Kawase 等人：组织培养。12. 298-303 (1986)

Harasawa, R. et al.: "A reliable and sensitive method for detecting mycoplasmas in cell cultures" Microbiology and Immunology. 30. 919-921 (1986)

Harasawa, R. 等人：“一种可靠且灵敏的检测细胞培养物中支原体的方法”微生物学和免疫学。

原澤亮 他: 日本獣医学雑誌. 47. 901-909 (1985)

Ryo Harasawa 等人：《日本兽医杂志》47. 901-909 (1985)

Harasawa,R.et al.: Microbiology and Immunology. 30. 919-921 (1986)

Harasawa,R.et al.：微生物学和免疫学。

Harasawa, R. et al.: "Genomic and phenotypic analyses of avian ureaplasmas." Japanese Journal of Veterinary Science. 47. 901-909 (1985)

Harasawa, R. 等人：“禽类解脲脲原体的基因组和表型分析。”