Diversity of functions and surface structures of Trypanosoma cruzi trypomastigotes

克氏锥虫锥鞭毛体功能和表面结构的多样性

• 批准号: 60480161
• 负责人: KANBARA Hiroji
• 金额: $ 2.3万
• 依托单位: Institute of Tropical Medicine, Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480161/
• 关键词: Trypanosoma cruzi; trypomastigote; virulence; surface antigen; 表面抗原; 表面構成成分; トリポマスティゴート

1. Virulence of Trypanosoma cruzi trypomastigotes is not caused by their penetrating abiliti but is dependent on their speed of the following steps. High-virulent trypomastigotes penetrated mouse macrophages or newly cultured fibroblasts from newborn mice at the almost same speed as low-virulent ones, but they promptly change form and start proliferation, while low-virulent ones require more than 48 hours lag time before proliferation. Tthis phenomenon is not found in the infectious experiment to the established fibroblasts (L-cells).2. There are more than 30 protein antigens on the cell surface of trypomastigote, none of which are prominent in quality and quantity. Thys, immune mouse sera can not agglutinate living trypomastigotes, even though they show considerable titer of indirect immunofluorescence to them.3. Lipid-like compoments predominantly appear on high-virulent trypomastigotes. Separated compoments from living trypomastigotes of different virulence by trypsin treatment are comparatively analysed on SDS-PAGE. White precipitates with a Mr range 37,000 to 50,000 were shown when the gels were fixed in 12.5% TCA but they disappeared when the samples were pre-treated with acetone.

1.克氏锥虫锥鞭毛体的毒力不是由其穿透能力引起的，而是取决于其后续步骤的速度。高毒力锥鞭虫以几乎与低毒力锥鞭虫相同的速度穿透小鼠巨噬细胞或新生小鼠新培养的成纤维细胞，但它们迅速改变形式并开始增殖，而低毒力锥鞭虫在增殖前需要超过48小时的滞后时间。在对已建立的成纤维细胞（L细胞）的感染实验中没有发现这种现象。2.锥鞭虫细胞表面有30多种蛋白抗原，但没有一种在质和量上是突出的。因此，免疫小鼠血清不能凝集活的锥鞭虫，即使它们对锥鞭虫显示出相当滴度的间接免疫荧光。类脂质成分主要出现在高毒力锥鞭毛体上。用胰蛋白酶处理不同毒力的活锥鞭毛体，在SDS-PAGE上对分离的组分进行了比较分析。当凝胶在12.5%TCA中固定时，显示出Mr范围为37，000至50，000的白色沉淀，但当样品用丙酮预处理时，它们消失。

项目成果

Kanbara, H., Fukuma T., Yanagi, T. & Nakazawa, S.: "Homogeneity of Trypamosoma cruzi strains of different virulence derived from a single strain" Tropical Medicine. 29 (2). 123-126 (1987)

神原 H.、福间 T.、柳 T.

Kanbara, H. & Nakabayashi, T.: "Comparative studies on surface antigenicity of Trypanosoma cruzi trypomastigotes from infected mouse blood and infected L-cell cultures" Biken Journal. 28. 71-77 (1985)

神原，H.

Hermosura, Ma. E.: "Comparative study on high and low virulent tryoimastigotes of Trypanosoma cruzi: Infectivity to mouse macrophages, L-cells and newly cultured fibtoblasts from mouse heart" Tropical Medicine. 27 (4). 211-219 (1985)

赫莫苏拉，马。

Kanbara, H., Yanagi, T., Nakazawa, S. & Fukuma, T.: "Comparison of virulent trypomastigotes with low-virulent ones derived from a single strain of Trypanosoma cruiz: infectivity to new fibroblasts from various organs of the mouse" Zbl. Bakt. Hyg. A. 264.

神原 H.、柳 T.、中泽 S.

神原廣二: Biken Journal. 28. 71-77 (1985)

神原浩二：Biken 杂志。28. 71-77 (1985)