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Developments of Rapid and Convenient New Diagnostic Method for Chagas' Disease.

快速、便捷的查加斯病新诊断方法的开发。

基本信息

批准号：
01870115
负责人：
KITAGAWA Tsunehiro
金额：
$ 4.8万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B).
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870115/
关键词：
Chagas' Disease New Diagnostic Method Trypanosoma Cruzi Epimastigote Trypomastigote Amastigote Immunoassay New Method

项目摘要

The Protozoan Trypanosoma cruzi, the etiologic agent of Chagas' disease, consist of a variety of different strains. There are 3 life-cycle forms of T. Cruzi. Epimastigotes multiply in the insect vector ; infectious trypomastigotes enter mammalian host cells and differentiate into amastigotes. Intracellular amastigotes multiply in host cells. The present study was aimed to develop enzyme immunoassay (EIA) methods for T. Cruzi of all three life-cycle forms. 1. EIA for T. Cruzi, trypomastigotes (a new diagnostic method for Chaga's disease) : Two EIA methods were developed : one is specific assay for Tulahuen strain and another one is general detection method for all strains of trypomastigotes. The later method is expected to be a useful diagnostic method for Chagas' disease, though its sensitivity should be improved. 2. The Eia for epimastigotes developed is a general assay for all strains of epimastigotes. The method could be a new diagnostic method for the detection of T. Cruzi infected insect vectors. 3. EIA specific to amastigotes of Tulahuen strain was developed. The method was successfully applied for the first detailed analysis of infection states of various organs of mice infected by T. Cruzi Tulahuen strain. In summary, we developed EIAs for all three life-cycle forms of T. Cruzi. All the methods were expected to be new useful diagnostic methods for various studies of Chagas' disease, some of which have already demonstrated.

原生动物克氏锥虫是查加斯病的病原体，由多种不同的菌株组成。克氏锥虫有3种生活史类型。表鞭毛体在昆虫媒介中繁殖；感染性锥体鞭毛体进入哺乳动物宿主细胞并分化为无鞭毛体。细胞内的无鞭毛体在宿主细胞中繁殖。本研究旨在建立克氏毛滴虫三种生活型的酶免疫分析(EIA)方法。1.克氏毛滴虫、鞭毛虫的EIA(查加氏病的一种新诊断方法)：建立了两种EIA方法：一种是针对图拉胡恩株的EIA方法，另一种是对所有株的锥虫的通用检测方法。后一种方法有望成为查加斯病的一种有用的诊断方法，但其敏感性有待提高。2.建立的上胚体EIA是对所有品系的上胚体进行的通用检测。该方法可作为检测克氏毛滴虫病媒的一种新的诊断方法。3.建立了图拉花品系无鞭毛体特异的EIA方法。该方法首次应用于克氏锥虫感染小鼠各器官感染状态的详细分析。总而言之，我们为克鲁兹毛滴虫的所有三种生活史形式制定了环境影响评估。所有这些方法都有望成为查加斯病的各种研究的新的有用的诊断方法，其中一些已经得到了证明。