ROLE OF SUPEROXIDE FROM MACROPHAGES IN HEPATIC FIBROSIS

巨噬细胞超氧化物在肝纤维化中的作用

• 批准号: 60480207
• 负责人: FUJIWARA Kenji
• 金额: $ 4.16万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480207/
• 关键词: Hepatic fibrosis; Collagen; Macrophage; Superoxide; アラビアゴム

The aim of this project is to investigate a role of superoxide from macrophages in the process of hepatic fibrosis.The following results have been obtained.1) When rats with hepatic fibrosis induced by dimethylnitrosamine administration received injections of arabic gum, a polysaccharide with hydroxyproline, macrophages swollen with vacuoles accumulated along increased hepatic fibers. Hepatic collagen synthesis was reduced after arabic gum adnimistration which stimulated rats to release superoxide from peritoneal macrophages. In case of other polysaccharides and latex particles, there was no such findings.2) Superoxide dismutase injections did not affect hepatic collagen synthesis in rats given dimethylnitosamine.From these, macrophages might play a role in inhibiting collagen synthsis in hepatic fibrosis. The significance of activated macrophages accumulated after arabic gum administration remains to be investigated. As the role of superoxide in collagen synthesis has not been proved in vivo, experiments in vitro should be carried out using a co-culture system of isolated hepatic fibroblasts with Kupffer cells from varying liver injuries.

本课题旨在研究巨噬细胞产生的超氧化物在肝纤维化过程中的作用，得到以下结果：1）二甲基亚硝胺诱导的肝纤维化大鼠，注射阿拉伯胶（一种含羟脯氨酸的多糖）后，巨噬细胞肿胀，空泡沿着增多的肝纤维聚集。阿拉伯胶刺激大鼠腹腔巨噬细胞释放超氧阴离子后，肝胶原合成减少。2）超氧化物歧化酶注射液对二甲基亚硝胺诱导的大鼠肝胶原合成无影响，巨噬细胞可能在抑制肝纤维化胶原合成中发挥作用。阿拉伯胶给药后激活的巨噬细胞积聚的意义仍有待研究。由于超氧化物在胶原合成中的作用尚未在体内得到证实，因此应使用分离的肝成纤维细胞与来自不同肝损伤的枯否细胞的共培养系统进行体外实验。

项目成果

Fujiwara,Kenji: in submission.

藤原贤二：服从。

Fujiwara,Kenji: "Irreversibility and treatment of hepatic fibrosis in rats.In:Pathobiology of hepatic fibrosis." Elsevier Science Publishers, 232 (1985)

Fujiwara，Kenji：“大鼠肝纤维化的不可逆性和治疗。见：肝纤维化的病理学。”

Ogata,Itsuro: Life Sciences. 37. 2269-2273 (1985)

绪方五郎：生命科学。

Fujiwara, K.: "Suppresion of hepatic fibrosis by arabic gum in rats intoxicated with dimethylnitrosamine: Possible role of activated macrophages" (in submission).

Fujiwara, K.：“阿拉伯胶对二甲基亚硝胺中毒大鼠肝纤维化的抑制：活化巨噬细胞的可能作用”（提交中）。

Kubota,Yasuo: British J.Dermatology. 113. 559-563 (1985)

久保田康夫：英国皮肤病学杂志。