ROLE OF SUPEROXIDE FROM MACROPHAGES IN HEPATIC FIBROSIS

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The aim of this project is to investigate a role of superoxide from macrophages in the process of hepatic fibrosis.The following results have been obtained.1) When rats with hepatic fibrosis induced by dimethylnitrosamine administration received injections of arabic gum, a polysaccharide with hydroxyproline, macrophages swollen with vacuoles accumulated along increased hepatic fibers. Hepatic collagen synthesis was reduced after arabic gum adnimistration which stimulated rats to release superoxide from peritoneal macrophages. In case of other polysaccharides and latex particles, there was no such findings.2) Superoxide dismutase injections did not affect hepatic collagen synthesis in rats given dimethylnitosamine.From these, macrophages might play a role in inhibiting collagen synthsis in hepatic fibrosis. The significance of activated macrophages accumulated after arabic gum administration remains to be investigated. As the role of superoxide in collagen synthesis has not been proved in vivo, experiments in vitro should be carried out using a co-culture system of isolated hepatic fibroblasts with Kupffer cells from varying liver injuries.