Photoradiation therapy of rat liver cancer - Protection of the normal liver by indocyanine green.

大鼠肝癌的光放射治疗——吲哚菁绿对正常肝脏的保护。

• 批准号: 60480211
• 负责人: ITOSHIMA Tatsuya
• 金额: $ 1.66万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480211/
• 关键词: Photoradiation therapy; Liver cancer; Hematoporphyrin derivatives; Indocyanine green; アルゴンレーザー

An application of photoradiation therapy to hepatocellular carcinoma (HCC) has been difficult because hematoporphyrin derivatives (HpD) accumulate not only in cancer cells but also in normal hepatocytes and, hence, laser irradiation causes injuries in both tissues. Protection of the normal liver tissue from laser phototoxicity was demonstrated using indocyanine green (ICG) as a protective agent. In vitro, argon laser irradiation decolored green tint of ICG much faster in solutions containing HpD than those without, suggesting that ICG captured excited oxygen from HpD. Degeneration of Chang hepatocytes induced by HpD and laser irradiation was protected by an addition of ICG into the medium. In vivo, laser irradiation of the rat liver surface caused hyperemia when HpD was injected two days before, while the hyperemia was much milder in rats added with ICG injection 10 minutes before the irradiation. ICG injected into rat HCC accumulated only in the normal liver tissue. Laser irradiation of rat HCC preinjected with both HpD and ICG destroyed only cancer tissue, preserving the surrounding liver tissue.Both in vitro and in vivo results suggest that ICG has a scavenger effect against excited oxygen and it might be used as a protective agent in photoradiation therapy of HCC.

由于血卟啉衍生物（HpD）不仅在癌细胞中而且在正常肝细胞中积累，因此，激光照射导致两种组织的损伤，因此光辐射治疗肝细胞癌（HCC）的应用一直是困难的。用吲哚菁绿色（ICG）作为保护剂，对正常肝组织的激光光毒性有保护作用。在体外，氩激光照射脱色绿色色调的ICG在溶液中含有HpD比那些没有更快，这表明ICG捕获HpD的激发氧。ICG对HpD和激光诱导的Chang肝细胞变性有保护作用。在体内，激光照射大鼠肝表面引起充血时，注射HpD两天前，而充血的大鼠在照射前10分钟加入ICG注射轻得多。注射到大鼠HCC中的ICG仅在正常肝组织中积累。激光照射预先注射HpD和ICG的大鼠肝癌，仅破坏癌组织，而保留周围肝组织，体内外实验结果表明ICG对激发态氧有清除作用，可作为光辐射治疗肝癌的保护剂。

项目成果

Kitak,et al: Gastroenterologia Japonica. 22. (1987)

Kitak 等人：日本胃肠病学。

Kita K, et al.: "Photoradiation therapy of rat liver cancer - Protection of the normal liver by indocyanine green." Gastroenterologia Japonica. 22. (1987)

Kita K 等人：“大鼠肝癌的光放射治疗 - 吲哚菁绿保护正常肝脏。”

喜田恵治ほか: 薬理と治療. 14. 353-354 (1986)

Keiji Kida 等人：药理学和治疗。14. 353-354 (1986)

Kita K, et al.: "An application of photoradiation therapy in experimentally induced rat hepatocellular carcinoma and protection of noncancerous parts by indocyanine green." Japanese Pharmacology & Therapeutics. 14. 353-354 (1986)

Kita K 等人：“光放射疗法在实验诱导的大鼠肝细胞癌中的应用以及吲哚菁绿对非癌部分的保护。”