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Dynamic Relationship between Transvascular Fluid Shift and Cardiac Output.

经血管液体转移与心输出量之间的动态关系。

基本信息

批准号：
60480350
负责人：
TANAKA Yoshifumi
金额：
$ 2.11万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

To investigate transvascular fluid shift under various pathophysiological states, a device for continuous measurement of circulating blood volume was developed. To use the dilution principle for measuring blood volume, Cr 51 tagged red blood cells were employed as a tracer and an extracorporeal shunt was made of the femoral artery and vein. Gamma ray counter was placed within the extracorporeal shunt. Counting time for the radioactivity was set at every 30 second and the data was transferred to microcomputer system. The accuracy of the blood volume determination was more than 98% with this system and it was sufficient to detect the movement of fluid shift through the vascular wall.Experiments with three different protocols were performed: 1) Carbon dioxide stress, 2) Total spinal block. 3) Nicardipine induced hypotension. To determine the transvascular filtration coefficient, the vascular compliance and the compliance of interstitial fluid space in whole body, lactate Ringer's solution was infused intravenously for 10 minutes at a rate of 1 ml/kg/min in the control phase and in those of stress phases.Circulatory parameters such as cardiac output, arterial pressure or venous pressure were almost same as reported by another investigators. However, significant changes in the relationship between blood volume and venous pressure were obtained. Calculated values of transvascular filtration coefficients and compliance of interstitial fluid space were almost same in each experiment. Compliance of vascularture were increased in Nicardipine group and decreased in carbon dioxide and total spinal block groups. Delayed compliance of vascularture were decreased in all stress stages. Hence, we conclude that the delayed compliance is regulated by autonomic nervous systems.

为了研究不同病理生理状态下经血管液体的转移，研制了一种循环血容量连续测量装置。为了使用稀释原理来测量血容量，使用Cr 51标记的红细胞作为示踪剂，并从股动脉和静脉制备体外分流器。伽马射线计数器放置在体外分流器内。放射性计数时间设定为每30秒一次，并将数据传输至微机系统。该系统的血容量测定的准确性大于98%，它是足够的，以检测通过血管壁的液体移位的运动。实验进行了三种不同的协议：1）二氧化碳应力，2）全脊髓阻滞。3)尼卡地平控制性降压。为测定经血管滤过系数、血管顺应性和全身间质液间隙顺应性，在对照期和应激期以1 ml/kg/min的速率静脉输注乳酸林格氏液10分钟，心输出量、动脉压或静脉压等循环参数与其他研究者报道的基本一致。然而，血容量和静脉压之间的关系的显着变化。经血管滤过系数和间质液间隙顺应性的计算值在各实验中基本相同。尼卡地平组血管顺应性增加，CO2组和全脊麻组血管顺应性降低。各应激阶段血管延迟顺应性均降低。因此，我们得出结论，延迟顺应性是由自主神经系统调节。