Isolation of Matrix Vesicles associating with Dentinogenesis and Application for Pulpotomy

矩阵载体是在矿化过程的早期阶段进入的，现在似乎大体上同意，因为它们是初始晶体形成的站点。目前的研究已携带这些容器在初始牙科发生后被孤立，并对其进行调查，以研究用于脉搏学的应用。Wistar大鼠的Apical Pulps被一种剃须刀刀片所刺激。它们的一小块被聚合酶孵化了。纸浆文摘是以中心为中心的。片段被发现如下： （1） 40，000 x g，20分钟; （2） 300，000 x g，10分钟。中心部位显示的接触细胞和细胞片段，以及部分片段1中部分片段的内塑性视网膜的电子显微镜。Vesicles Closey重新装配矩阵Vesicles，因为它们的体积小，电子密集的内容，集中在第二部分。碱性磷酸酶的特定活动为4.57 <+! 0.76 U. U.总蛋白质含量为0.73 <+!>0.10克<mu>/毫克湿重。含有ATP （ 2 - 5小时， 37 ° C ）溶液的基质载体中的类似Needle晶体并没有生长，这些结果表明基质载体首先从牙科纸浆具有生物活性和类似的精细结构到体内。进一步的研究将有必要在体外形成基质载体中的羟基伴侣;关于孵化介质和文化条件的内容可能是决定性因素。这一未来的调查可以捕捉到基本数据，将这些仪器应用于脉冲学。

Matrix vesicles are involved in the early stages of the mineralization process, and it now seems to be generally agreed that they are sites of initial crystal formation. Present study was carried out to isolate these vesicles during initial dentinogenesis and to investigate the application for pulpotomy.Apical pulps of Wistar rats were excised with a razor blade. Small slices of them were incubated with collagenase. Pulp digest was centrifuged. Fractions were obtained as follows : (1) 40,000 x g for 20 min ; (2) 300,000 x g for 10 min. Electron microscopy of the centrifugal sediments showed intact cells and cell fragments, and partially fragmented endoplasmic reticulum in fraction 1. Vesicles closely resembling the matrix vesicles, because of their small size, electron-dense contents, were concentrated in the 2nd fraction. The specific activity for alkaline phosphatase was 4.57 <+!-> 0.76 U. Total protein contents were 0.73 <+!-> 0.10 <mu> g /mg wet weight. Needle-like crystals did not grow within matrix vesicles in Ca・P solution containing ATP ( 2 - 5 h, 37゜C ).These results indicate that matrix vesicles first isolated from dental pulp has biological activity and similar fine structure to that of in vivo ones. Further study would be necessory to form the hydroxyapatite in matrix vesicles in vitro; the contents of incubation medium and culture condition might be decisive factors. This future investigation could catch the basic data to applicate these vesicles for pulpotomy.