SEROEPIDEMIOLOGICAL AND VIROLOGICAL STUDIES OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS(LCMV) IN LABORATORY ANIMALS.

实验动物中淋巴细胞脉络膜脑膜炎病毒 (LCMV) 的血清流行病学和病毒学研究。

• 批准号: 60480485
• 负责人: SATO HIROSHI
• 金额: $ 3.26万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480485/
• 关键词: Lymphocytic choriomeningitis virus; LCM virus; Rodents; 間接蛍光抗体

INDIRECT FLUORESCENT ANTIBODY METHOD WAS APPLIED FOR A DETECTION OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS(LCMV) ANTIBODY IN COLONIES OF LABORATOREY ANIMALS IN JAPAN. THE RESULTS SHOWED THAT THE ANTIBODY EXIST IN SPF MICE(3/152, 2.2%) AND CONVENTIONAL MICE(30/539, 5.6%) WITH LOW TITERS RANGING FROM 1:10 TO 1:160. THE ANTIBODY WAS ALSO DETECTED IN 2.2%(2/89) OF SYRIAN HAMSTERS, AND 2.9%(2/68) OF APODEMUS AGRARIUS, 21.4%(3/14) OF JAPANESE HARVEST MICE WHICH HAVE BEEN MAINTAINED AS LABORATORY COLONY FOR SEVERAL YEARS. FURTHERMORE, 8.6%(19/222) ANTIBODY POSITIVE ANIMALS WITH COMPARATIVE TITERS WERE DETECTED IN THE SEVERAL SPECIES OF RODENTS CAPTURED IN WESTERN PARTS OF JAPAN. HOWEVER, THE ANTIBODY WAS NOT DEMONSTRATED IN THE SERA OF MONGOLIAN GERBIL, SUNCUS MURINUS, GUINEA PIG AND RAT, THUS FAR. THESE RESULTS SUGGEST THAT LCMV INFECTION IS PRESENT IN LABORATORY ANIMALS AND WILD RODENTS IN JAPAN AS WELL AS WESTERN COUNTRIES, AND POINTED OUT THE IMPORTANCE OF MICROBIOLOGICAL MONITORING FOR LCMV IN LABORATORY AND WILD RODENTS. A TRIAL OF VIRUS ISOLATION WAS RESULTED IN NEGATIVE. REISOLATION STUDY OF LCMV FROM WILD RODENTS SHOULD BE CONDUCTED IN NEAR FUTURE.

应用间接荧光抗体法检测日本实验动物群体中的淋巴细胞性脉络膜脑膜炎病毒（LCMV）抗体。结果表明，SPF级小鼠（3/152，2.2%）和普通级小鼠（30/539，5.6%）均存在抗体，抗体滴度较低，在1：10 ~ 1：160之间。在实验室饲养多年的叙利亚仓鼠、黑线姬鼠和日本收获鼠中，抗体检出率分别为2.2%（2/89）、2.9%（2/68）和21.4%（3/14）。此外，在日本西部捕获的几种啮齿动物中，检出了8.6%（19/222）的抗体阳性动物，其效价相当。但在蒙古沙鼠、豚鼠、豚鼠和大鼠血清中均未检测到该抗体。这些结果表明，在日本和西方国家的实验室动物和野生啮齿动物中存在LCMV感染，并指出了实验室和野生啮齿动物中LCMV微生物监测的重要性。病毒分离试验结果为阴性。应尽快开展野生啮齿动物LCMV的再分离研究

项目成果

佐藤浩: モダンメディア. 32. 384-394 (1986)

佐藤宏：现代媒体。32。384-394（1986）

Hiroshi Sato: "Physicochemical inactivation of zoonotic viruses: Hemorrhagic fever with renal syndrome(HFRS) and lymphocytic choriomeningitis(LCM) viruses." Susceptibility of laboratory animal viruses to disinfectants or physical treatments.:

Hiroshi Sato：“人畜共患病毒的物理化学灭活：肾综合征出血热（HFRS）和淋巴细胞性脉络膜脑膜炎（LCM）病毒。”

佐藤浩: ラボラトリーアニマル. 3. 3-27 (1986)

佐藤宏：实验动物。3. 3-27 (1986)

佐藤浩: "微生物検査必携ウイルス・クラミジア・リケッチア検査第3版" 日本公衆衛生協会, (1987)

Hiroshi Sato：“病毒、衣原体和立克次体的基本微生物检测，第 3 版”日本公共卫生协会，（1987 年）

Hiroshi Sato: "Laboratory diagnosis for lymphocytic choriomeningitis caused with viral agent."

Hiroshi Sato：“病毒制剂引起的淋巴细胞性脉络膜脑膜炎的实验室诊断。”