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Studies on paralytic shellfish poison if "hiogi" scallop

“hiogi”扇贝麻痹性贝毒的研究

基本信息

批准号：
61480070
负责人：
HASHIMOTO Kanehisa
金额：
$ 3.78万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Studies on paralytic shellfish poison in "hiogi" scallop Chlamys nobilis1) Composition of paralytic shellfish poison (PSP) in "hiogi" scallopAn extract from toxic digestive glands of hiogi scallop Chlamys nobilis, was subjected to column chromatography on Bio-Gel P-2 and Bio-Rex 70 (H^+ form), resulting in separation into three fractions: protogonyautoxin (PX), gonyautoxin (GTX) and saxitoxin (STX) fractions.Their total potencise were estimated to be <100 mouse units (MU), 3,200 MU and 3,700 MU, respectively. Electrophoretic and thin-layer chromatographic analyses disclosed that PX fraction consisted mainly of PX_1 and PX_2, GTX fraction of GTX_5 and GTX_6, along with two unidentified toxins, and STX fraction exclusively of another two unidentified toxins. Some attempts were made to identify the major one of the four unidentified toxins by high performance liquid chromatography and electrophoresis, demonstrating its identity to decarbamoyl STX. 2) Bioconversion of oyster PSP components into decarbamoyl STX by a hiogi visceral homogenate Toxins consisting mainly of low-toxic PSP components were partially purified from toxic oyster. When the toxins were added to a hiogi visceral homogenate and incubated, the low-toxic components were found to be converted into decarbamoyl STX. However, the homogenate did not show such a bioconversion of PSP when a scallop toxin consisting mainly of high-toxic components was uwed instead. In addition, the homogenate lost almost completely the converting ability when heated, or treated with acid or ethanol, suggesting that some enzyme(s) present in the hiogi visceral homogenate is involved in the bioconversion of PSP.

栉孔扇贝麻痹性贝毒的研究1)栉孔扇贝麻痹性贝毒(PSP)的组成用Bio-Gel P-2和Bio-Rex 70(H^+型)柱层析从栉孔扇贝有毒消化腺中提取液，得到3个组分：原藻毒素(Px)、藻毒素(GTx)和岩藻毒素(STx)，其总势分别为100、3,200和3,700鼠单位(MU)。电泳和薄层分析表明，PX组分主要由PX_1和PX_2组成，GTX组分由GTX_5和GTX_6组成，另有两种未知毒素，STX组分仅由另外两种未知毒素组成。用高效液相色谱和电泳法对四种未知毒素中的主要一种进行了鉴定，证明了其与脱氨甲酰STX的同源性。2)利用HIGI内脏匀浆将牡蛎PSP组分生物转化为脱氨基甲酰STX。毒素主要由低毒PSP组分组成。当这些毒素被加入Hiogi内脏匀浆中并孵育时，发现低毒成分被转化为脱氨基甲酰STX。然而，当以高毒组分为主的扇贝毒素被加入时，匀浆中没有显示出这种PSP的生物转化。此外，匀浆在加热、酸或乙醇处理时几乎完全失去了转化能力，这表明Hiogi内脏匀浆中存在的某种酶(S)参与了PSP的生物转化。