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Chemical structures of carbohydrate epitopes of host cell membrane recognized by fimbriae of pathogenic bacteria

病原菌菌毛识别的宿主细胞膜碳水化合物表位的化学结构

基本信息

批准号：
61480081
负责人：
NAIKI Masaharu
金额：
$ 4.35万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

1. Uropathogenic E. coli P fimbriae -- E. coli with P fimbriae which recognize Gal -4Gal sequence of CTH(P^k antigen), globoside (P antigen) and P_1 glycolipid (P_1 antigen) were frequently isolated from human pyelonephitis urines. We identified that some E. coli strains with same P fimbrial antigen recognize Forssman glycolipid but not any Pantigenic glycolipid and other strains recognize potently Forssman glycolipid and weakly CTH and globoside. These fimbrial adhesin was different from P adhesin.2. Enterotoxigenic E. coli K99 fimbrial--K99 fimbrial E. coli is a causative agent of calf and piglet diarohea. This fimbrial adhesin was found to recognize N-glycolylneuraminyl-lactosylceramide, GM_3(NeuGc) of host intestinal mucosa. GM_3(NeuGc) was detected in intestinal mucosa of newborn calf and piglet but it completely disappeared at the 2nd week after birth. The adhesin factor was concentrated in recombinant bacteria by recombination DNA technique. It became applicable for the fimbrial vaccination.3. Corynebacterium renale fimbriae--C. renale is a causative agent of bovine pyelonephritis. The bacteria adhere to urinary track epitherium with their fimbriae. We prepared monoclonal antibodies to the fimbriae, which can inhibit the adhesion to host cells. The monoclonal antibody recognized 48000 fimbrial protein. Recobination DNA technique alsp showed that 1.4K bp of C. renale chromosomal DNA codes the same protein product.

1.尿路致病大肠杆菌P菌毛--含有识别Cth(P^k抗原)、Globoside(P抗原)和P_1糖脂(P_1抗原)半乳糖半乳糖(Gal-4Gal)序列的菌毛的大肠埃希菌，常从人肾盂肾炎尿液中分离到。我们发现具有相同P菌毛抗原的一些菌株识别Forssman糖脂而不识别任何抗原性糖脂，而另一些菌株对Forssman糖脂有较强的识别能力，而对Cth和Globoside的识别较弱。这些菌毛粘附素不同于P粘附素。产肠毒素大肠杆菌K99菌毛--K99菌毛是引起犊牛和仔猪腹泻的病原体。这种菌毛粘附素能识别宿主肠粘膜上的N-糖神经氨基-乳糖基神经酰胺GM_3(NeuGc)。GM_3(NeuGc)在新生小牛和仔猪的肠粘膜中可检测到，但在出生后2周完全消失。利用重组DNA技术在重组菌中浓缩粘附素因子。适用于菌毛疫苗接种。肾棒状杆菌菌毛--C.肾炎是牛肾盂肾炎的一种病原体。细菌通过菌毛附着在尿路上皮上。我们制备了抗菌毛的单抗，可以抑制与宿主细胞的黏附。该单抗可识别48000菌毛蛋白。DNA重组技术ALSP分析表明，肾小球藻染色体DNA的1.4kbp编码相同的蛋白质产物。