Studies on the human glomerulonephritis inducing antigenic molecules using new nephritis models and monoclonal antibodies.

用EHS肉瘤或Fx 1A免疫诱导的膜性肾病大鼠脾细胞与大鼠骨髓瘤细胞Y-3融合，制备了自身反应性单克隆抗体（MoAb）。单次或混合注射与近端肾小管刷状缘或肾小球上皮细胞表面反应的单克隆抗体，免疫荧光显示可导致系膜沉积物的形成，但不导致任何肾损伤，胶原酶消化抗原和百日咳杆菌辅助的强烈和持续免疫，这两种免疫新试验成功地产生了许多有效结合肾小球的单克隆抗体。将代表性IgGl MoAb 5-1-6一次性静脉内注射到大鼠中诱导大量蛋白尿。免疫电镜显示，单克隆抗体5-1-6在体外结合肾小球上皮足突的表面，主要是狭缝隔膜。免疫沉淀法测得的抗原分子量约为51 Kd。该单克隆抗体为蛋白尿发病机制的研究提供了一个有价值的工具。

Splenocytes from rats with membranous nephropathy which was induced by the immunization of EHS sarcoma or Fx1A, were fused with rat derived myeloma, Y-3 and some autoreactive monoclonal antibodies (MoAb) were produced. The single and mixed injections of MoAbs reactive with proximal tubular brush border or glomerular epithelial cell surface, lead to mesangial deposits formation demonstrated with immunofluorescence but not to any renal lisions.Digestion of antigen with collagenase and intense and persistent immunization with the aids of Bordetella pertussis, these two new trials in immunization resulted in success in producing effectively many glomeruli binding MoAbs. One shot intravenous injection of the representative IgGl MoAb, 5-1-6, into rats induced massive proteinuria. Immunoelectron microscopy revealed that MoAb 5-1-6 binds in vitro to the surface of glomerular epithelial foot process, mainly to slit diaphragms. The molecular weight of antigenic molecules was about 51 Kd by immunoprecipitation. This MoAb provides a valuable tool for research into the mechanism of proteinuria.