ANALYSIS OF PURE PANCREATIC JUICE IN PANCREATIC DISEASES AND ITS CLINICAL SIGNIFICANCE

我们用EIA法测定乳铁蛋白(LF)水平，同时测定内窥镜下胰液(PJ)中的常规参数，并评估PJ中的蛋白堵塞(PP)。此外，还检测了PJ中与癌症相关的碳水化合物抗原，并使用抗体对胰腺标本进行免疫组织化学染色。结果表明，46%的慢性胰腺炎(CP)患者PJ中LF水平异常升高，其中钙化组(82%)显著高于非钙化组(37%)。此外，随着CP程度的加重，PJ中的LF水平也随之升高。由于PP在55%的CP患者中出现，而在除胰腺癌(8%)以外的其他疾病患者中未发现PP，PP被认为是CP的相对特异性。钙化CP组PP发生率(82%)高于非钙化CP组(55%)。而胰腺异常与胰腺功能的显著差异是无…。PP的存在与不存在之间存在更多的t。PP阳性组CP组PJ低频异常均值及发生率均高于PP阴性组。CA19-9在胰腺癌(PC)和胰腺癌(CP)中的表达水平均显著高于胰腺癌(PC)和CP，其发生率分别为92%和85%。PJ中的CA19-9被认为是非特异性胰腺损伤的敏感指标，但对肿瘤不敏感。PJ中ST-439和SLX浓度仅在PC中显著升高，其患病率分别为57%和40%。它们具有较高的肿瘤特异性，但发病率相对较低。糖链抗原CA19-9和CA-50在胰腺癌中表达较强，但特异性较低。而CSLEX-1和SLX等2型碳水化合物抗原的肿瘤特异性最高，但其发病率低于其他抗原。ST-439和DU-PAN-2具有较高的阳性和特异性。CA19-9主要分布于CP的增生期小胰管。这可能反映了CP患者PJ中CA19-19水平的升高。较少

We determined lactoferrin (LF) levels by EIA, as well as conventional parameters in endoscopically aspirated pancreatic juice (PJ), and evaluated the protein plug (PP) in PJ. Furthermore, cancer-associated carbohydrate antigens in PJ were measured, and immunohistochemical staining of pancreatic specimens was performed using antibodies. The results are summerzied as follows.Abnormaly high levels of LF in PJ were observed in 46% of the patients with chronic pancreatitis (CP), with the prevalence being significantly higher in calcified CP 82%) than in non-calcified CP (37%). Moreover, LF levels in PJ rose with the severity of CP. As PP in PJ was found in 55% of the patients with CP,but not in those with other diseases except for pancreatic cancer (8%), PP was considered to be relatively specific for CP. PP in PJ was observed more frequently in calcified CP (82%) than in non-calcified CP (55%). However, significant differences of abnormalires in pancreatogam and pancreatic function were no … More t observed between presence and absence of PP. Mean value and pevalence of abnormal LF in PJ in CP were higher in the PP positive group than in the PP-negative group. Concentration of CA 19-9 in PJ was significantly higher not only in pancreatic cancer (PC) but also in CP, and its prevalence was 92% in PC and 85% in CP. CA 19-9 in PJ in pj was considered to be a sensitive marker for non-specific pancreatic injury, but not for tumors. Concentrations of ST-439 and SLX in PJ were significantly increased only in PC, and their prevalences were 57% and 40%, respectively. They have high tumor-specificity, but their incidence was relatively low. CA 19-9 and CA-50, which are the type 1 chain carbohydrate antigens, were strongly expressed in pancreatic cancer, but were low in cancerspecificity. However, type 2 chain carbohydrate antigens such as CSLEX-1 and SLX showed highest tumor-secificity among the antigens, whereas their incidences were lower than other antigens. On the other hand, ST-439 and DU-PAN-2 exhibited high positivity and specificity. CA 19-9 was strongly and widely distributed mainly in the proliferative small pancreatic duct of CP. This may reflect the increased levels of CA 19-19 in PJ of patients with CP. Less