Analysis of the mechanism of chemical carcinogenesis and short-term screening of tumor-promoters using clonogenic a ssay

化学致癌机制分析及克隆形成试验短期促癌筛选

基本信息

  • 批准号:
    61480340
  • 负责人:
  • 金额:
    $ 3.2万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1986
  • 资助国家:
    日本
  • 起止时间:
    1986 至 1987
  • 项目状态:
    已结题

项目摘要

Colony formation in soft agar was observed with bladder cells prior to papilloma development in rats treated with N- Butyl-N-(4-hydroxybutyl)nitrosamine(BBN), and the number of colonies formed was dependent on the quantity of BBN consumed. It was then demonstrated that sodium saccharin and several amino acids, which are known to act as rat bladder cancer promoters, induce colony formation of rat bladder cells after treatment with subcarcinogenic dose of BBN, suggesting that there may be a correlation between tumor-promoting activity and colony inducing activity.Effect of anti-tumor-promoter on colony formation was then assessed in two-stage mouse skin carcinogenesis experiment. Soft agar colony formation of mouse epidermal cells induced by 12-0- tetradecanoylphorbol-13-acetate(TPA) after single application of 7,12-dimethylbenz[a]anthracene(DMBA) was inhibited by treatment of skin with retinoic acid. Effect of dietary retinyl acetate. nordihydroguaiaretic acid(NDGA) and quinacrine hydrochloride on soft agar colony formation of rat bladder cells was also eaaluated. Soft agar colony formation of bladder cells induced by sodium saccharin after treatment with subcarcinogenic dose of BBN was inhibited by the administration of retinyl acetate or NDGA. suggesting the possible anti-tumor-promoting activity of the two agents.Thus, an application of clonogenic assay to carcinogenesis experiment could be useful to analyse early events in carcinogenesis and provide a short-term screening method for detecting new tumor-promoters and anti-tumor-promoters.
在用N-丁基-N-(4-羟丁基)亚硝胺(BBN)处理的大鼠中,在乳头状瘤发展之前,用膀胱细胞在软琼脂中观察到集落形成,并且形成的集落数量取决于消耗的BBN的量。结果表明,糖精钠和几种氨基酸具有促膀胱癌作用,亚致癌剂量的BBN可诱导大鼠膀胱癌细胞集落形成,提示促癌活性与集落诱导活性之间可能存在相关性,并在两阶段小鼠皮肤癌变实验中评价抗促癌剂对集落形成的影响。用维甲酸处理皮肤可抑制7,12-二甲基苯并[a]蒽(DMBA)单独作用后12-0-十四酰佛波醇-13-乙酸酯(TPA)诱导的小鼠表皮细胞软琼脂集落形成。饮食中的醋酸视黄酯的影响。去甲二氢愈创木酸(NDGA)和盐酸奎纳克林对大鼠膀胱细胞软琼脂集落形成的影响。用亚致癌剂量的BBN处理后,糖精钠诱导的膀胱细胞软琼脂集落形成被给予醋酸视黄酯或NDGA抑制。因此,将克隆形成实验应用于肿瘤发生实验中,可用于分析肿瘤发生的早期事件,并为发现新的肿瘤促进剂和抗肿瘤促进剂提供一种短期的筛选方法。

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takayuki Hashimura: "Soft agar colony formation of bladder cells during carcinogenesis induced by N-Butyl-N-(4-hydroxybutyl)nitrosamine and application to detection of bladder cancer promoters" Japanese Journal of Cancer Research(Gann). 78. 473-479 (1987)
Takayuki Hashimura:“N-丁基-N-(4-羟丁基)亚硝胺诱导的致癌过程中膀胱细胞的软琼脂集落形成及其在膀胱癌促进剂检测中的应用”日本癌症研究杂志(江恩)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takayuki Hashimura: Japanese Journal fo Cancer Research(Gann). 78. 473-479 (1987)
Takayuki Hashimura:日本癌症研究杂志(江恩)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
橋村孝幸: Japanese Journal of cancer Research(Gann). 76. 321-323 (1985)
Takayuki Hashimura:日本癌症研究杂志(江恩)76。321-323(1985)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
橋村孝幸: Japanese Journal of cancer Research(Gann).
Takayuki Hashimura:日本癌症研究杂志(江恩)。
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    0
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YOSHIDA Osamu其他文献

Federalism and Peace: A Comparative Analysis of Indian and South-East Asian Experiences
联邦制与和平:印度和东南亚经验的比较分析
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ohsaki;Noriko;YOSHIDA Osamu
  • 通讯作者:
    YOSHIDA Osamu
Negative origin of a cultural trait? Myths of the loss of literacy
文化特征的负面起源?
  • DOI:
    10.31250/2618-8600-2019-3
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ohsaki;Noriko;YOSHIDA Osamu;宇根義己;和崎聖日;Hitoshi Yamada
  • 通讯作者:
    Hitoshi Yamada
How Local Politics Matters? Academic and Practical Potentials of Comparative Studies between and within India, Japan and Post-Conflict Countries
当地政治如何重要?
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sumarto Bayu K A;Kobayashi Hirozumi;Kakioka Ryo;Tanaka Rieko;Maeda Ken;Tran Hau D;Koizumi Noriyuki;Morioka Shinsuke;Bounsong Vongvichith;Watanabe Katsutoshi;Musikasinthorn Prachya;Tun Sein;Yun L K C;Anoop V K;Raghavan Rajeev;Masengi Kawilarang W A;Fujimot;坂田隆;YOSHIDA Osamu
  • 通讯作者:
    YOSHIDA Osamu
日本市場向けアパレル生産のインド展開―品質管理と商慣行に着目して―
在印度扩大面向日本市场的服装生产 - 注重质量控制和商业实践 -
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ohsaki;Noriko;YOSHIDA Osamu;宇根義己
  • 通讯作者:
    宇根義己

YOSHIDA Osamu的其他文献

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{{ truncateString('YOSHIDA Osamu', 18)}}的其他基金

Basic Structure of the International Legal Order for the Global Atmosphere
全球大气国际法律秩序的基本结构
  • 批准号:
    22330015
  • 财政年份:
    2010
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Grant-in-Aid Research Project on Differentiation and its Theories The Alternative Path to Peace and Cooperation
差异化及其理论资助研究项目和平与合作的另类道路
  • 批准号:
    20310148
  • 财政年份:
    2008
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The US-Japan Security Treaty Regime and the Asia-Pacific Nations
美日安保条约体系与亚太国家
  • 批准号:
    09620070
  • 财政年份:
    1997
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Nationwide survey of urolithiasis in Japan
日本全国尿石症调查
  • 批准号:
    08457422
  • 财政年份:
    1996
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Establishment of criteria and technique for molecular genetical diagnosis of urothelial cancer
尿路上皮癌分子遗传学诊断标准和技术的建立
  • 批准号:
    06454457
  • 财政年份:
    1994
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Study on the induction of cytotoxic lymphocytes that act on urological tumors and its clinical application
泌尿系肿瘤细胞毒性淋巴细胞的诱导及其临床应用研究
  • 批准号:
    03454385
  • 财政年份:
    1991
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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Molecular and pathological understanding of oral early cancer using mouse chemical carcinogenesis model
使用小鼠化学致癌模型对口腔早期癌的分子和病​​理学理解
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用生药或汉方药预防化学癌的可能性
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Studies in Chemical Carcinogenesis
化学致癌研究
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    7679024
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Studies in Chemical Carcinogenesis
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Immunofluorescence imaging analysis for intra- and inter-cellular signal transduction mechanisms in chemical carcinogenesis models
化学致癌模型中细胞内和细胞间信号转导机制的免疫荧光成像分析
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BRIN: URI: TMSR/CHEMICAL CARCINOGENESIS SUBCORE
BRIN:URI:TMSR/化学致癌子核心
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    6973513
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