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Human Urinary Cytology Using Secondary and Back-Scattered Electron Image

使用二次和背散射电子图像进行人类尿细胞学检查

基本信息

批准号：
61480341
负责人：
OHMORI Hiroyuki
金额：
$ 2.88万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

A new method of human urinary cytology using secondary and back-scattered electron modes was studied. 1) Urinary exfoliated cells were examined by a direct mapping technique, i.e., a combined use of light (LM) and scanning electron microscopy (SEM). Malignancy of exfoliated urothelial cells has been determined on the basis of the presence or absence of pleomorphic microvilii as observed by secondary electron mode SEM. Subsequently, a new "SEM grading" system for human urinary cytology was proposed. 2) Informations obtained by back-scattered electron mode SEM were less valuable than those obtained by LM for human urinary cytology. 3) Applicability of immunological labeling method to this system was examined. T4-bacteriophage and gold-granule were used as marker and blood group ABO antigens and bladder cancer-specific antigens were labeled. These labels were clearly detercted on urinary exfoliated urothelial cells both by secondary and back-scattered electron modes. However, back-scattered electron mode using gold-granule was superor to secondary electron mode using T4-bacteriophage or gold-granule for shortening the time for detecting labeled cells under SEM. Therefore, a combined use of back-scattered electron image using gold-granule as immuno-marker for the presence or absence of antigens with secondary electron image for surface morphology has been proved to be a most promising method for SEM cytology. We belive that the present methods for SEM cytology is far most superior to conventional methods for detecting low grade papillary cancer and evaluating malignant potential of exfoliated cancer cells.

研究了一种利用二次和背向散射电子模式进行人体尿液细胞学检查的新方法。1)采用光镜和扫描电子显微镜相结合的直接标测技术对尿脱落细胞进行检测。尿脱落细胞的恶性程度是根据二次电子扫描电子显微镜观察到的多形性微血管的存在与否而确定的。随后，提出了一种新的人体尿液细胞学“扫描电子显微镜分级”系统。2)背散射电子扫描对人体尿液细胞学的诊断价值不如光镜检查。3)对免疫标记方法在该系统中的适用性进行了考察。以T4噬菌体和金颗粒为标记物，标记ABO血型抗原和膀胱癌特异性抗原。这些标记通过二次和背散射电子模式在尿脱落的尿路上皮细胞上被清楚地检测到。而金颗粒的背散射电子模式优于T4噬菌体或金颗粒的二次电子模式，从而缩短了扫描电子显微镜下标记细胞的检测时间。因此，以金颗粒为免疫标记物的背散射电子像与表面形态的二次电子像相结合是扫描电子显微镜细胞学最有前途的方法。我们认为，目前的扫描电子显微镜细胞学方法在检测低度乳头状癌和评估脱落癌细胞的恶性潜能方面远远优于传统方法。