Patholory of dental implants A cellular pathological study of responses to non-antigenic foreign body

牙种植体的病理学 对非抗原性异物反应的细胞病理学研究

• 批准号: 61480374
• 负责人: ISHIKI Tetsuo
• 金额: $ 4.29万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480374/
• 关键词: foreign body; macrophage; foreign body giant cell; hydroxyapatite; 合成ハイドロキシアパタイト; インプラント

Recently, dental implantology has been developed and many kind of biomaterials were supllied and used in clinically. However, we did not have sufficient knowlege for the fundamental mechanism of host's foreign body recognition.This study was planned to clarify tissue and cell responses to implant materials ( biomaterial ), the particle size was prepared as large as cell size.Time course histopathological observation of lesions induced by subcutaneous injections of fine ceramic particles of synthetic hydroxyapatite, ranging 0.65 - 30 microns,was performed by light and electron microscopy. This particle is not soluble in water, in other word, inactive and non-antigenic foreign body.The summary of the result is; non-specific foreigh body reaction was observed through the period of experiment. The smaller-sized particles, under 7microns indiameter, were phagocytized by macrophages and larger-sized or coagulated particles were surrounded by multinucleated giant cells. We demonstrated the maturation course of macrophage and suggested that macrophages fused into foreign body giant cells and glycocalyx of cell surface play an important role in the recognition of foreign body in the process of phagocytosis.We concluded that foreign body granuloma formation was observed even biocompatible apatite ceramic particles. We abvise to take notice about in long-term clinical applications of dental implants.

近年来，口腔种植学得到了发展，各种生物材料被广泛应用于临床。然而，我们对宿主识别异物的基本机制缺乏足够的了解，本研究旨在阐明组织和细胞对植入材料的反应通过皮下注射0.65 - 30微米范围的合成羟基磷灰石的细陶瓷颗粒诱导的损伤的时程组织病理学观察，通过光学和电子显微镜进行。该颗粒不溶于水，即无活性和非抗原性异物。结果总结为：在整个实验期间观察到非特异性异物反应。直径小于7 μ m的小颗粒被巨噬细胞吞噬，大颗粒或凝固颗粒被多核巨细胞包围。我们观察了巨噬细胞的成熟过程，认为巨噬细胞在吞噬过程中与异物巨细胞融合，细胞表面的糖萼在异物识别中起重要作用，即使是生物相容性的磷灰石陶瓷颗粒也能形成异物肉芽肿。因此，种植牙在临床上的长期应用应引起重视。

项目成果

Shin-ichi Shimizu: "Subcutaneous tissue responses in rats to injection of fine particles of synthetic hydroxyapatite ceramic." Biomedical Research. 9. 95-111 (1988)

Shin-ichi Shimizu：“注射合成羟基磷灰石陶瓷细颗粒后大鼠皮下组织的反应。”

森雅美: 歯科基礎医学会雑誌. 29. (1987)

森雅美：日本基础牙科医学会杂志 29。（1987）

富井康年: 新潟歯学会雑誌. 17. 65-82 (1987)

富井康稔：《新泻牙科学会杂志》17. 65-82 (1987)

Yasutoshi Tomii: "Subcutaneous tissue reactions of the rat to synthetic hydroxyapatite ceramic particles." Niigata Dental Journal. 17. 65-82 (1987)

Yasutoshi Tomii：“大鼠对合成羟基磷灰石陶瓷颗粒的皮下组织反应。”

森雅美: 新潟歯学会雑誌. 17. 99-115 (1987)

森雅美：新泻牙科学会杂志 17. 99-115 (1987)