Experimental and clinical studies on oncogenesis of salivary glands.

对本院手术切除的70例涎腺肿瘤进行免疫组织化学染色。S-100a免疫组织化学染色主要见于多形性腺瘤。多形性腺瘤的二重体结构保留了乳铁蛋白和分泌成分的产生功能，与功能分化一致。这一结果支持了Regezi关于涎腺肿瘤起源的双细胞学说。对低级别分化粘液表皮样肿瘤移植到裸鼠皮下的组织病理学特征进行了研究。结论黏液表皮样肿瘤的黏液分泌细胞可由表皮样细胞和中间细胞分化或转化。为阐明涎腺细胞的后天分化，对大鼠颌下腺进行了超微结构和形态计量学研究。在唾液腺发育过程中，大鼠颌下腺末梢小管细胞逐渐减少，出生后近6周基本消失，腺泡细胞在此期间大量增加。超微结构显示唾液腺末梢小管细胞未转化为腺泡细胞。用肌动蛋白荧光探针NBD-Pallacidin观察了大鼠舌下腺肌上皮细胞的形态分化。在新生儿，腺泡周围可见弥漫荧光的细胞，代表初生肌上皮细胞。出生后3~4天，肌动蛋白染色集中在平行于细胞突起长轴排列的链上。出生后10d，肌上皮细胞进一步发育，突起数量和厚度增加。出生后60d，肌上皮细胞发育完全。它们是星状的，包围着个体的腺泡。

70 cases of salivary gland tumors removed surgically in our hospital were stained immunohistochemically. Positive immunostaining for S-100 a was observed in pleomorphic adenoma specifically. Duet-like structure in pleomorphic adenoma retains the function of production of lactoferrin and secretory component in accordance with functional differentiation. This results supported Regezi's bicellular theory on the origin of salivary gland tumor.The low grade differentiated mucoepidermoid tumor, transplanted subcutaneously to nude mouse, was studied on these histophathological feature. We concluded that mucous secreting cells of mucoepidermoid tumor would be differentiated or transformed from the epidermoid cells and the intermediated cells.To elucidate the postnatal differentiation of salivary gland cells, rat submandibular gland were examined ultrastructurally and morphometrically. During the development of salivary glands, terminal tubule cells of rat submandibular gland decreased gradually and disappeared almost 6 weeks after birth, acinar cells increased greatly in this period. Ultrastructural finding suggested that terminal tubule cells of salivary gland do not transform into acinar cells. Furthermore, late weaning resulted in marked developmental delay of the gland.Morphological dfferentiation of myoepitherlial cells of rat sublingual gland was examined by using the fluorescent probe for actin, NBD-phallacidin. In newborn, cells with diffuse fluoresecence occurred around the acini, representing incipient myoepithelial cells. Between 3 and 4 days after birth, actin staining was concentrated in strands which were arranged parallel to the long axis of the cell processes. Myoepithelial cells had developed further by 10 days after birth with an increased number and thickness of their processes. Fully developed myoepithelial cells were found on day 60 after birth. These were stellate and encompassed individual acini.