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Experimental and clinical studies on oncogenesis of salivary glands.

唾液腺肿瘤发生的实验和临床研究。

基本信息

批准号：
61480415
负责人：
TANIOKA Hiroaki
金额：
$ 3.65万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

70 cases of salivary gland tumors removed surgically in our hospital were stained immunohistochemically. Positive immunostaining for S-100 a was observed in pleomorphic adenoma specifically. Duet-like structure in pleomorphic adenoma retains the function of production of lactoferrin and secretory component in accordance with functional differentiation. This results supported Regezi's bicellular theory on the origin of salivary gland tumor.The low grade differentiated mucoepidermoid tumor, transplanted subcutaneously to nude mouse, was studied on these histophathological feature. We concluded that mucous secreting cells of mucoepidermoid tumor would be differentiated or transformed from the epidermoid cells and the intermediated cells.To elucidate the postnatal differentiation of salivary gland cells, rat submandibular gland were examined ultrastructurally and morphometrically. During the development of salivary glands, terminal tubule cells of rat submandibular gland decreased gradually and disappeared almost 6 weeks after birth, acinar cells increased greatly in this period. Ultrastructural finding suggested that terminal tubule cells of salivary gland do not transform into acinar cells. Furthermore, late weaning resulted in marked developmental delay of the gland.Morphological dfferentiation of myoepitherlial cells of rat sublingual gland was examined by using the fluorescent probe for actin, NBD-phallacidin. In newborn, cells with diffuse fluoresecence occurred around the acini, representing incipient myoepithelial cells. Between 3 and 4 days after birth, actin staining was concentrated in strands which were arranged parallel to the long axis of the cell processes. Myoepithelial cells had developed further by 10 days after birth with an increased number and thickness of their processes. Fully developed myoepithelial cells were found on day 60 after birth. These were stellate and encompassed individual acini.

本文对我院70例涎腺肿瘤手术切除标本进行免疫组化染色。S-100 a在多形性腺瘤中特异性表达.多形性腺瘤中的Duet样结构保留了与功能分化一致的乳铁蛋白和分泌成分的产生功能。本实验对低分化粘液表皮样瘤裸鼠皮下移植瘤的组织病理学特征进行了研究。结论粘液表皮样瘤的粘液分泌细胞是由表皮样细胞和中间细胞分化或转化而来的。在大鼠涎腺发育过程中，颌下腺终小管细胞逐渐减少，生后6周左右消失，腺泡细胞在此期间大量增加。超微结构显示，涎腺终末小管细胞不转化为腺泡细胞。用肌动蛋白荧光探针NBD-鬼笔菌素（phallacidin）观察了大鼠舌下腺肌上皮细胞的形态学差异。在新生儿，细胞弥漫性荧光出现在腺泡周围，代表早期肌上皮细胞。在出生后3和4天之间，肌动蛋白染色集中在平行于细胞突起长轴排列的链中。出生后10天，肌上皮细胞进一步发育，其突起数量和厚度增加。出生后60天发现完全发育的肌上皮细胞。这些是星状的，包含单个腺泡。