Immunochemical studies of the mammalian egg coat and immunosuppressive roles of decidual cells in implantation

哺乳动物卵膜的免疫化学研究和蜕膜细胞在植入中的免疫抑制作用

• 批准号: 61480435
• 负责人: MORI Tsuneatsu
• 金额: $ 3.2万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480435/
• 关键词: Zona pellucida; Decidual cell; 免疫抑制作用; 胱溶膜細胞; 精子レセプター; 子宮内脱落膜細胞; 免疫抑制物質; 発癌遺伝子

In this project, molecular mechanisms, which promoted the species specific and/or allogeneic recognition between sperm and egg,were investigated. On the other hand, implantation, which was the naturally succeeded semiallograft, was studied on the immunosuppressive functions of decidual tissues. By using the monoclonal technology, the monoclonal antibody detecting the cross reactive portion between human and porcine zona pellucida(ZP) was obtained and its portion was chemically defined as protein nature. The carbohydrate structure of ZP specifically binding with porcine sperms was analysed and then finished in neutral oligosaccharides. Further study on its acidic oligosaccharides was started. The interaction of class II MHC molecules on sperm and L3T4 like molecules on egg vitellus in mouse system was strongly suggested as the mechanisms for the allogeneic recognition in both gamates.It was revealed that supernatant derived from human decidual cell line demonstrated the activity of immunosuppression but also the inhibition of tumor cell line growth and the enhancement of mouse embryonal development in vitro. The chemical analysis and purification corresponding with biological assay are now going under study.

在该项目中，研究了促进精子和卵子之间的物种特异性和/或同种异体识别的分子机制。另一方面，植入是自然成功的半同种异体移植，研究了蜕膜组织的免疫抑制功能。利用单克隆技术，获得了检测人与猪透明带（ZP）交叉反应部分的单克隆抗体，并将该部分化学定义为蛋白质性质。分析了与猪精子特异性结合的ZP的碳水化合物结构，并最终制成中性寡糖。对其酸性低聚糖进行了进一步的研究。小鼠系统中精子上的 II 类 MHC 分子与卵黄体上的 L3T4 样分子的相互作用被强烈认为是两种配子中同种异体识别的机制。结果表明，来自人蜕膜细胞系的上清液表现出免疫抑制活性，还具有抑制肿瘤细胞系生长和增强体外小鼠胚胎发育的活性。与生物测定相应的化学分析和纯化正在研究中。

项目成果

Mori,E.: "On the epitopes of porcine zona pellucida recognized by two monoclonal antibodies" Eur. J. Obstet. Gyn. and Reprod.in press. (1989)

Mori,E.：“关于两种单克隆抗体识别的猪透明带的表位”Eur。

Mori.T;Yamauchi.K;Iwakura.Y;Toyoda.Y: "Manipulating the Mouse Embryo" 近代出版, 332 (1988)

Mori.T；Yamauchi.K；Iwakura.Y；Toyoda.Y：“操纵小鼠胚胎”Kindai Shuppan，332 (1988)

Tatsumi,K.: A.J.Reprod.Immunol.and Microbiol.(1987)

Tatsumi,K.：A.J.Reprod.Immunol.and Microbiol.(1987)

Tatsumi,K.: A.J.Reprod.Immunol.and Microbiol.13. 87-92 (1987)

Tatsumi,K.：A.J.Reprod.Immunol.and Microbiol.13。

Mori,T.: "Clinical significance of autoantibodies to the zona pellucida,in the mammalian egg coat.Dietl J.ed." SpringerーVerlag, 111-127 (1989)

Mori, T.：“哺乳动物卵壳中透明带自身抗体的临床意义。Dietl J.ed. Springer-Verlag，111-127 (1989)”