Immunochemical studies of the mammalian egg coat and immunosuppressive roles of decidual cells in implantation

本课题主要研究促进精子与卵子之间种属特异性和/或同种异体识别的分子机制。另一方面，研究了自然成功的半同种异体移植物--种植体对蜕膜组织免疫抑制功能的影响。利用单克隆技术，获得了检测人与猪透明带交叉反应部位的单克隆抗体，其化学成分为蛋白质。对与猪精子特异性结合的ZP进行了糖结构分析，并以中性寡糖的形式进行了结构修饰。对其酸性低聚糖的进一步研究已开始。在小鼠系统中，精子上的II类MHC分子和卵卵黄上的L3 T4类分子的相互作用是两种配子识别同种异体抗原的机制，人蜕膜细胞培养上清具有免疫抑制活性，同时也具有抑制肿瘤细胞生长和促进小鼠胚胎发育的作用。与生物测定相适应的化学分析和纯化方法正在研究中。

In this project, molecular mechanisms, which promoted the species specific and/or allogeneic recognition between sperm and egg,were investigated. On the other hand, implantation, which was the naturally succeeded semiallograft, was studied on the immunosuppressive functions of decidual tissues. By using the monoclonal technology, the monoclonal antibody detecting the cross reactive portion between human and porcine zona pellucida(ZP) was obtained and its portion was chemically defined as protein nature. The carbohydrate structure of ZP specifically binding with porcine sperms was analysed and then finished in neutral oligosaccharides. Further study on its acidic oligosaccharides was started. The interaction of class II MHC molecules on sperm and L3T4 like molecules on egg vitellus in mouse system was strongly suggested as the mechanisms for the allogeneic recognition in both gamates.It was revealed that supernatant derived from human decidual cell line demonstrated the activity of immunosuppression but also the inhibition of tumor cell line growth and the enhancement of mouse embryonal development in vitro. The chemical analysis and purification corresponding with biological assay are now going under study.