Basic studies on sperm-egg binding and applications to contraception

精卵结合的基础研究及其在避孕中的应用

• 批准号: 06556057
• 负责人: MORI Tsuneatsu
• 金额: $ 4.35万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06556057/
• 关键词: sp38; sperm; zona pellucida; contraception; 避妊ワクチン; 精子蛋白; Sp38; 卵子透明帯糖鎖; 硫酸化糖鎖; 透明帯結合蛋白質

We founs a novel sperm protein sp38 with zona pellucida (ZP)-binding activity located at the anterior portion of many mammalian species. It was originally purified from boar epididymal sperm as a 38 kDa glycoprotein. The cDNA clones encoding sp38 was isolated from boar testis cDNA library based on the results of partial amino acid sequences of purified protein. The cDNA encoded a 350-residue amino acid precursor protein which was posttranslationally modified into a mature protein of 299 amino acid residues. Northern blot analysis demonstrated that the transcript of sp38 was expressed only in the testis. Mouse and human homologues of boar sp38 was obtained from testis sDNA libraries of respective species and showed highly homologous sequences. Boar sp38 bound to 90kDa-ZP glycoprotein species in a calcium-dependent manner. The binding characteristics of sp38 to the ZP were similar to those of proacrosin. Antibodies against sp38 inhibited the binding of sperm to the ZP in vitro egg-sperm binding assay.

我们发现了一个新的精子蛋白sp38与透明带（ZP）的结合活性，位于许多哺乳动物的前部。它最初是从猪附睾精子中纯化的38 kDa糖蛋白。根据纯化蛋白的部分氨基酸序列分析结果，从猪睾丸cDNA文库中克隆了编码sp38的cDNA克隆。该cDNA编码一个350个氨基酸残基的前体蛋白，该前体蛋白经后修饰为一个299个氨基酸残基的成熟蛋白。北方印迹分析表明，sp38的转录本只在睾丸中表达。猪sp38的小鼠和人类同源物从各自物种的睾丸sDNA文库中获得，并显示高度同源的序列。猪sp38以钙依赖性方式与90 kDa-ZP糖蛋白结合。sp38与ZP的结合特征与顶体蛋白原的结合特征相似。在体外卵-精子结合试验中，抗sp38抗体抑制精子与ZP的结合。

项目成果

Takasaki,S.,Mori,T.et.al: "Sturctures of sugar chains included in porcine zona pellucida glycoproteins" J.Reprod.Dev.39. 39-40 (1993)

Takasaki,S.,Mori,T.et.al：“猪透明带糖蛋白中包含的糖链结构”J.Reprod.Dev.39。

Guo, MW., Mori, T., et al.: "Identification of Fas antigen associated with apoptotic cell death in murine overy" Biochem. Biophys. Res. Commun.203. 1438-1446 (1994)

Guo, MW.、Mori, T. 等人：“鉴定与小鼠过度凋亡细胞死亡相关的 Fas 抗原”Biochem。

Mori T et al: "Moleucular mechanisms in mammalian ferilazation" J.Mamm.Ova.Res.(in press). (1997)

Mori T 等人：“哺乳动物受精的分子机制”J.Mamm.Ova.Res.（出版中）。

Xu,JP.,Mori,T.et.al: "Expression of Fas ligand mRNA in murine ovary" J.S.I.R.10. 63-64 (1996)

Xu,JP.,Mori,T.et.al：“小鼠卵巢中 Fas 配体 mRNA 的表达”J.S.I.R.10。

高崎誠一(分担): "グリコバイオロジーシリーズ第5巻、グリコテクノロジー" 講談社, 27 (1994)

高崎精一（撰稿人）：“糖生物学系列第 5 卷，糖技术”讲谈社，27（1994 年）