Reproductive Immunological Research for Oogenesis, Oomortality, Fertilization and Implantation

卵子发生、卵死亡率、受精和着床的生殖免疫学研究

• 批准号: 11470344
• 负责人: MORI Tsuneatsu
• 金额: $ 4.99万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470344/
• 关键词: Soern; Egg; Fas; FasL system; Apoptosis; CD5γHLA-DR* NS cell; Apoptosis-inducing nucleosides; DNA strand break; Caspase-3; FasL系; CD57^+HLA-DR^<bright>NS細胞; DNA strand break; 精巣; 卵巣; 受精; 胎盤脱落膜; Fas-FasL; Apoptosis Inducing Nucleoside

The ovarian follicular arresia is one of typical phenomenoes in programmed cell death. We have recently demonstrated that the definite expression of Fas was found in ovarian oocytes, ovarilated eggs and grandosa cells in maturated follicle, while, Fas legend (L) was restrictedly expressed in grandosa cells under the * regulation of gonadotropins. Therefore, we concluded that Fas -FasL system display a primary role to promote ovarian atresia through apoptosis, most likely depending on local gonadotropin levels in the ovary. In marine tests, we have revended that Fas is expressed in testicular germ cells and FasL is expressed in sertoli cells indicating their molecular interactions during the spermatogenesis. Based on the analysis of all or partial sugar structurer os porcine or marine zona pellucida (ZP), we found that marine or porine sperm bound to β-Galactose rather than α-Galactose residue and/or Le^x residue on egg ZP. We are now cloning the gene of β-Galactose binding protein from … More porcine tests. We have further confirmed the expression and function of CD4/p56* complex on marine egg plasma membrane corresponding to the presence of MHC class 11 molecule at the posteriox region of sprm. The decidua-derived CD57-HLA-DR* natural suppressor (57.DR-NS) cell line relenses a series of active factors into the culture to generate the apoptosis of human malignant cells and trophoblast cells. Actually, 57-DR-NS cell line generated the apoptosis in human * (Molt4/K562) and gastric / esophageal / prostatix or chorionic carcinoma (GCIY / T.Tn / PC-3 /BeWo) cells but not in a human diploid normal (W1-38) cells. The chemical structures of the active factors were determined as a series of modified nucleasides. They could generate the apoptotic cell death of Molt4/K562 and GCIY/T.Tn/PC-3/BeWo malignant cells following by DNA strand breaks and caspase-3 activation, but not that of WI-38 normal cells at all. The administration of them into GCIY/Molt4 tumor bearing SCID mice resulted in the drastic suppression of turnor growth followed by the decrease in tunor size due to the occurrence of apoptosis in * tissues. Less

卵泡发育迟缓是细胞程序性死亡的典型现象之一。最近的研究表明，Fas在卵巢卵母细胞、卵巢成熟的卵子和卵泡中的颗粒细胞中有明确的表达，而在促性腺激素的调节下，Fas legend（L）仅在颗粒细胞中表达。因此，我们得出结论，Fas -FasL系统显示通过凋亡促进卵巢闭锁的主要作用，最有可能依赖于卵巢局部促性腺激素水平。在海洋实验中，我们发现Fas在睾丸生殖细胞中表达，FasL在支持细胞中表达，表明它们在精子发生过程中存在分子相互作用。通过对猪或海洋透明带（ZP）的全部或部分糖结构的分析，发现海洋或猪精子与卵ZP上的β-半乳糖结合，而不是α-半乳糖残基和/或Le^x残基。我们目前正在克隆β-半乳糖结合蛋白基因， ...更多信息 猪试验我们进一步证实了CD 4/p56* 复合物在海洋卵质膜上的表达和功能，其对应于SPRM后区MHC class 11分子的存在。来源于蜕膜的CD 57-HLA-DR* 天然抑制细胞系（57.DR-NS）释放一系列活性因子进入培养物中，引起人类恶性肿瘤细胞和滋养层细胞的凋亡。实际上，57-DR-NS细胞系在人 *（Molt 4/K562）和胃/食管/结肠癌或绒毛膜癌（GCIY / T.Tn / PC-3 /BeWo）细胞中产生凋亡，但在人二倍体正常（W1-38）细胞中不产生凋亡。活性因子的化学结构确定为一系列修饰的核苷。它们能诱导Molt 4/K562和GCIY/T.Tn/PC-3/BeWo恶性肿瘤细胞DNA链断裂和caspase-3激活后的凋亡性细胞死亡，而对WI-38正常细胞无明显影响。将它们给药于荷GCIY/Molt 4肿瘤的SCID小鼠导致肿瘤生长的急剧抑制，随后由于组织中发生凋亡而导致肿瘤大小减小。少

项目成果

Li, X., Xu, J.P., Guo, M.W., Mori, E., Mori, T.: "Apoptotic cell death of huma gastric numor induced by nucleosides from CDSγHLA-DR* natural suppressor cell line"International Journal of Oncology. 14. 687-694 (1999)

Li，X.，Xu，J.P.，Guo，M.W.，Mori，E.，Mori，T.：“CDSγHLA-DR*天然抑制细胞系核苷诱导的人胃细胞凋亡”国际肿瘤学杂志14。 .687-694 (1999)

森 庸厚, 郭 卯戊: "卵細胞のアポトーシス,(鈴木秋悦、佐藤英明 編) 卵子研究法"養賢堂. pp.251-pp.256 (2001)

森恒笃、郭五福：“卵细胞凋亡，（铃木昭吉、佐藤英明编）卵母细胞研究方法”Yokendo，第251-256页（2001年）。

Li,X.,Mori,T., et al: "Activation of caspase-3 in Molt4 cell by apoptosis inducing nucleosides from CD57^+HLA-DR^<bright> natural suppressor cell line."Biochem.Biophys.Res.Communn.. 268. 514-520 (2000)

Li,X.,Mori,T. 等人：“通过诱导 CD57^HLA-DR^<bright> 天然抑制细胞系的核苷在 Molt4 细胞中激活 caspase-3。”Biochem.Biophys.Res.Communn。

森 庸厚: "卵子.(岩波講座「現代医学の基礎」第五巻[生殖と発生])"(岩波書店). 214 (1999)

Tsuneatsu Mori：“卵子。（岩波讲座《现代医学基础》第 5 卷 [生殖与发育]）”（岩波书店）214（1999）。

Xn, J.P., Li, X., Mori, E., Goo, M.W., Miasuda, I., Takaichi, H., Amano, T., Mori, T.: "Expression of Fas-Fas ligend in marine *"American Journal of Reproductive *. 42. 382-388 (1999)

Xn，J.P.，Li，X.，Mori，E.，Goo，M.W.，Miasuda，I.，Takaichi，H.，Amano，T.，Mori，T.：“Fas-Fas配体在海洋中的表达*”美国